Expression of hPOT1 in HeLa Cells and the Probability of Gene Variation of hpot1 Exon14 in Endometrial Cancer are Much Higher than in Other Cancers

Liu, Fei; Pu, Xiaoyun; Huang, Shaoguang; Xiang, Guiming; Jiang, Dongneng; Hou, Gou; Di-nan, Huang

doi:10.7314/apjcp.2012.13.11.5659

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…POT1 expression was higher in the biopsy tissue of cervical cancer patients than that in normal cervical smears. In previous studies, POT1 had high and strong expression in HeLa cells (12). Consistent with this, POT1 also had high expression in the biopsy tissue of cervical cancer patients, as well the HeLa cell line.…”

Section: Discussionsupporting

confidence: 86%

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Profile of POT1 as telomerase shelterin component discriminatesbetween cervical cancer and normal cervical cells

Wijaya¹,

Hidayatullah²,

Setyabudhi³

et al. 2017

Turk J Med Sci

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Background/aim: Telomerase activity is influenced by hTERT transcriptional regulation, shelterin, and posttranscriptional alternative splicing. Telomerase shelterin such as POT1 is highly correlated with various cancers. However, the profile of POT1 in cervical cancer has not been clearly identified. Therefore, it is important to identify its profile in cervical cancer biopsy tissue and normal cervical smears.Materials and methods: Biopsy tissue of cervical cancer patients and normal cervical smears were characterized using SDS-PAGE and western blot. Sixteen biopsy tissues of cervical cancer patients and 15 normal cervical smears were measured for POT1 level using ELISA. Results:The inline band at 70 kDa indicated that all samples had protein that was identified as POT1. Western blot showed that telomerase antibody only recognized POT1 in biopsy tissue of cervical cancer patients. There was a significant difference (P = 0.01) in POT1 level between biopsy tissue of cervical cancer patients and normal cervical smears. Conclusion:POT1 was identified at 70 kDa in biopsy tissue of cervical cancer patients and its level was higher than that in normal cervical smears. The high level of POT1 in the biopsy tissue of cervical cancer patients showed the influence of this shelterin component in cervical carcinogenesis and also cell immortalization.

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Section: Discussionsupporting

confidence: 86%

“…Consistent with this, POT1 also had high expression in the biopsy tissue of cervical cancer patients, as well the HeLa cell line. Expression of POT1 has been identified in some cancer cell lines such as gastric cell lines SGC-7901 and MKN28 and larynx squamous carcinoma cell lines Hep-2 and Hep-2R (12).…”

Section: Discussionmentioning

confidence: 99%

Profile of POT1 as telomerase shelterin component discriminatesbetween cervical cancer and normal cervical cells

Wijaya¹,

Hidayatullah²,

Setyabudhi³

et al. 2017

Turk J Med Sci

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“…The protein and/or mRNA levels of the most conserved out of all shelterin proteins, POT1 (26) were increased in many different cancers including gastric, thyroid, breast (199, 278, 279) and in endometrial cancers (280). Higher levels of point mutations in the POT1 gene were observed in endometrial cancers, revealing that genetic variations in POT1 may lead to carcinogenesis in the endometrium (280). Simultaneous conditional inactivation of the shelterin protein POT1a with the tumor suppressor p53 in endometrial epithelial cells in a murine model, induced type II metastatic adenocarcinomas in 100% of the animals by 15 months (281).…”

Section: Endometriummentioning

confidence: 99%

Telomerase and Telomeres in Endometrial Cancer

et al. 2019

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Telomeres at the termini of human chromosomes are shortened with each round of cell division due to the “end replication problem” as well as oxidative stress. During carcinogenesis, cells acquire or retain mechanisms to maintain telomeres to avoid initiation of cellular senescence or apoptosis and halting cell division by critically short telomeres. The unique reverse transcriptase enzyme complex, telomerase, catalyzes the maintenance of telomeres but most human somatic cells do not have sufficient telomerase activity to prevent telomere shortening. Tissues with high and prolonged replicative potential demonstrate adequate cellular telomerase activity to prevent telomere erosion, and high telomerase activity appears to be a critical feature of most (80–90%) epithelial cancers, including endometrial cancer. Endometrial cancers regress in response to progesterone which is frequently used to treat advanced endometrial cancer. Endometrial telomerase is inhibited by progestogens and deciphering telomere and telomerase biology in endometrial cancer is therefore important, as targeting telomerase (a downstream target of progestogens) in endometrial cancer may provide novel and more effective therapeutic avenues. This review aims to examine the available evidence for the role and importance of telomere and telomerase biology in endometrial cancer.

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“…Thus, we also selected shelterin proteins TERF1/2 for examination. The third shelterin subunit, POT1 was not included as it has already been examined in the endometrium [ 47 ]. TERF2IP was included in our study as it binds to TERF2, therefore is structurally relevant [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Telomere and Telomerase-Associated Proteins in Endometrial Carcinogenesis and Cancer-Associated Survival

Button

Rogers

Thomas

et al. 2022

IJMS

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Risk of relapse of endometrial cancer (EC) after surgical treatment is 13% and recurrent disease carries a poor prognosis. Research into prognostic indicators is essential to improve EC management and outcome. “Immortality” of most cancer cells is dependent on telomerase, but the role of associated proteins in the endometrium is poorly understood. The Cancer Genome Atlas data highlighted telomere/telomerase associated genes (TTAGs) with prognostic relevance in the endometrium, and a recent in silico study identified a group of TTAGs and proteins as key regulators within a network of dysregulated genes in EC. We characterise relevant telomere/telomerase associated proteins (TTAPs) NOP10, NHP2, NOP56, TERF1, TERF2 and TERF2IP in the endometrium using quantitative polymerase chain reaction (qPCR) and immunohistochemistry (IHC). qPCR data demonstrated altered expression of multiple TTAPs; specifically, increased NOP10 (p = 0.03) and reduced NHP2 (p = 0.01), TERF2 (p = 0.01) and TERF2IP (p < 0.003) in EC relative to post-menopausal endometrium. Notably, we report reduced NHP2 in EC compared to post-menopausal endometrium in qPCR and IHC (p = 0.0001) data; with survival analysis indicating high immunoscore is favourable in EC (p = 0.0006). Our findings indicate a potential prognostic role for TTAPs in EC, particularly NHP2. Further evaluation of the prognostic and functional role of the examined TTAPs is warranted to develop novel treatment strategies.

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Expression of hPOT1 in HeLa Cells and the Probability of Gene Variation of hpot1 Exon14 in Endometrial Cancer are Much Higher than in Other Cancers

Cited by 4 publications

References 29 publications

Profile of POT1 as telomerase shelterin component discriminatesbetween cervical cancer and normal cervical cells

Profile of POT1 as telomerase shelterin component discriminatesbetween cervical cancer and normal cervical cells

Telomerase and Telomeres in Endometrial Cancer

Telomere and Telomerase-Associated Proteins in Endometrial Carcinogenesis and Cancer-Associated Survival

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