Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells

Zhang, Xufang; Jiang, Hongwei; Gong, Qimei; Fan, Chen; Huang, Yihua; Ling, Junqi

doi:10.1016/j.bbrc.2014.07.027

Cited by 19 publications

(18 citation statements)

References 31 publications

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“…It is therefore reasonable to speculate that hypoxia in dental pulp could induce the cytoplasmic expression and release of HMGB1, initiating the immune response. Recent study has confirmed the expression of HMGB1 and its receptor RAGE in adult dental pulp fibroblasts (Sugars et al 2007) and the cytoplasmic expression of HMGB1 as well as increased mRNA expression of HMGB1 and RAGE were also observed in inflamed pulp tissues compared to that in healthy dental pulp (Zhang et al 2014). Taken together, our results that showed the increased expression of HMGB1 in dental pulp fibroblasts are consistent with the inflammatory status during orthodontic tooth movement and previous reports.…”

Section: Discussionsupporting

confidence: 95%

Expression of HMGB1 in the periodontal tissue subjected to orthodontic force application by Waldo’s method in mice

Feng

et al. 2014

J Mol Hist

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Recent studies indicate that high mobility group box protein 1 (HMGB1) originating from periodontal ligament (PDL) cells can be a potential regulator in the process of orthodontic tooth movement and periodontal tissue remodeling. The aim of this study is to investigate HMGB1 expression in periodontal tissue during orthodontic tooth movement in mice according to Waldo's method. Six 7-week-old C57BL6 mice were used in these experiments. The elastic band was inserted into the teeth space between the right first and second maxillary molars. After 3 days of mechanical loading, mice were fixed with transcardial perfusion of 4 % paraformaldehyde in 0.1 M phosphate buffer (pH 7.4), and the maxillary was extracted for histochemical analyses. The histological examination revealed local PDL tear at the tension side and the formation of extensive cell-free hyaline zones at the compression side. The immunolocalization of HMGB1 was significantly presented at tension side of PDL, apical area and dental pulp, whereas at the compression side of PDL, the labeling of HMGB1 was almost undetectable as the presence of hyaline zone. Taken together, we concluded that the orthodontic tooth movement by Waldo's method leads to histological changes and HMGB1 expression pattern that differ from those of coil spring method, including PDL tear and extensive hyaline zone which may severely destroy periodontal tissue and in turn impede tooth movement.

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Section: Discussionsupporting

confidence: 95%

Expression of HMGB1 in the periodontal tissue subjected to orthodontic force application by Waldo’s method in mice

Feng

et al. 2014

J Mol Hist

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“…HMGB1 plays an important role in promoting tissue regeneration after acute inflammation. Locally released HMGB1 recruits bone-marrow derived mesenchymal stem cells (MSCs), and promotes the proliferation and differentiation of tissue-associated resident stem cells, such as dental pulp stem cells, mesoangioblasts, and MSCs ( 63 ). Adult MSCs have attracted intense interest because they can be isolated from the bone marrow and can be expanded in culture while maintaining their multipotency, and thus may be used for the repair of bone, cartilage, muscle, bone marrow stroma, tendon, fat, and other connective tissues.…”

Section: Hmgb1 and Atp In Tissue Repairmentioning

confidence: 99%

DAMPs from Cell Death to New Life

Véneréau¹,

Ceriotti²,

2015

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Our body handles tissue damage by activating the immune system in response to intracellular molecules released by injured tissues [damage-associated molecular patterns (DAMPs)], in a similar way as it detects molecular motifs conserved in pathogens (pathogen-associated molecular patterns). DAMPs are molecules that have a physiological role inside the cell, but acquire additional functions when they are exposed to the extracellular environment: they alert the body about danger, stimulate an inflammatory response, and finally promote the regeneration process. Beside their passive release by dead cells, some DAMPs can be secreted or exposed by living cells undergoing a life-threatening stress. DAMPs have been linked to inflammation and related disorders: hence, inhibition of DAMP-mediated inflammatory responses is a promising strategy to improve the clinical management of infection- and injury-elicited inflammatory diseases. However, it is important to consider that DAMPs are not only danger signals but also central players in tissue repair. Indeed, some DAMPs have been studied for their role in tissue healing after sterile or infection-associated inflammation. This review is focused on two exemplary DAMPs, HMGB1 and adenosine triphosphate, and their contribution to both inflammation and tissue repair.

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“…To start with, DPSC need to be mobilized from their niche. In the literature, various DPSC mobilization signals have been reported which include growth factors (hepatocyte growth factor (HGF), basic fibroblast growth factor (FGF-2), transforming growth factor (TGFβ-1)), chemokines (monocyte chemoattractant protein-1 (MCP-1); chemokine (C-X-C motif) ligand 14 (CXCL14); stromal cell-derived factor 1 (SDF-1)), granulocyte-colony stimulating factor (G-CSF), sphingosine-1-phosphate (S1P), C5a and high mobility group box 1 (HMGB-1) [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. These signals find their origin in different sources, including their release from the dentin matrix and their production by dental pulp cells.…”

Section: Migration Of Dpsc In Injured Pulp Microenvironmentmentioning

confidence: 99%

“…Another example of a molecule traditionally known for its role in inflammation and with discovered chemotactic potential is HMGB-1. It is secreted in inflammatory conditions by dendritic cells, macrophages and monocytes, and increases the production of inflammatory cytokines [ 14 ]. HMGB-1 expression was observed in inflamed pulp tissue, both in the nuclear and cytoplasmic regions of fibroblasts, inflammatory and vascular endothelial cells.…”

Section: Influence Of Inflammationmentioning

confidence: 99%

“…A chemotactic effect of HMGB-1 was observed with a reorganization of F-actin that accumulated in the cell periphery of dental pulp cells. Accordingly, the receptor that mediates the chemotactic effect of HMGB-1 was upregulated as well in inflamed dental pulp tissues [ 14 ]. In addition, HMGB-1 has also been shown to promote the proliferation and odontoblastic differentiation of DPSC [ 55 ].…”

Section: Influence Of Inflammationmentioning

confidence: 99%

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Dental Pulp Stem Cell Recruitment Signals within Injured Dental Pulp Tissue

et al. 2016

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The recruitment of dental pulp stem cells (DPSC) is a prerequisite for the regeneration of dentin damaged by severe caries and/or mechanical injury. Understanding the complex process of DPSC recruitment will benefit future in situ tissue engineering applications based on the stimulation of endogenous DPSC for dentin pulp regeneration. The current known mobilization signals and subsequent migration of DPSC towards the lesion site, which is influenced by the pulp inflammatory state and the application of pulp capping materials, are reviewed. The research outcome of migration studies may be affected by the applied methodology, which should thus be chosen with care. Both the advantages and disadvantages of commonly used assays for investigating DPSC migration are discussed. This review highlights the fact that DPSC recruitment is dependent not only on the soluble chemotactic signals, but also on their interaction with neighboring cells and the extracellular matrix, which can be modified under pathological conditions. These are discussed to explain how these modifications lead to the stimulation of DPSC recruitment.

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Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells

Cited by 19 publications

References 31 publications

Expression of HMGB1 in the periodontal tissue subjected to orthodontic force application by Waldo’s method in mice

Expression of HMGB1 in the periodontal tissue subjected to orthodontic force application by Waldo’s method in mice

DAMPs from Cell Death to New Life

Dental Pulp Stem Cell Recruitment Signals within Injured Dental Pulp Tissue

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