Expression of death receptor 3 (DR3) on peripheral blood mononuclear cells of patients with psoriasis vulgaris

Abstract

BackgroundA series of previous reports indicated that tumour necrosis factor-like ligand 1A (TL1A) and its receptor death receptor 3 (DR3) are involved in the pathogenesis of psoriasis vulgaris (PV), which is a common chronic skin disease accompanied by a number of comorbidities, although their exact roles remain unclear. Our previous studies demonstrated that serum TL1A levels were substantially elevated in patients with PV, but the detection of DR3 expression in peripheral blood mononuclear cells (PBMCs) of patients with PV had not been reported. Therefore, we detected DR3 expression on CD4+, CD8+, CD14+ and CD19+ PBMCs of patients with PV, atopic dermatitis (AD) and healthy volunteers.MethodsBlood samples were collected from participants with PV before and after treatment. Then, PBMCs from patients with PV were isolated. The Psoriasis Area Severity Index (PASI) was used to assess severity in patients with PV. The DR3 on CD4+, CD8+, CD14+ and CD19+ PBMCs were detected by flow cytometry analysis. Pearson’s correlation analysis was then used to investigate the relationship between DR3 expression and PASI scores in patients with PV.ResultsComparing with the healthy volunteers and patients with AD, the percentage of DR3-expressing on CD8+ and CD14+ PBMCs in patients with PV was elevated, but the percentage of DR3-expressing on CD8+ and CD14+ cells decreased after anti-inflammatory treatment, which was correlated with PASI scores.ConclusionsTaken together, these findings suggest that DR3 may play a key role in the pathogenesis of PV.

Concentration of Na+-taurocholate-cotransporting polypeptide expressed after in vitro-transcribed mRNA transfection determines susceptibility of hepatoma cells for hepatitis B virus

“…Introduction and overexpression of proteins at comparable levels in different cell lines is an important step to elucidate their cellular function or impact towards permissivity for a pathogen 3 , 28 , 36 – 38 . Currently, viral or plasmid vectors are most frequently used to introduce certain genes into cells facing several restrictions.…”

Expression of Death Receptor 3 (DR3) on Circulating CD4 Positive Lymphocytes on Patients with Vitiligo

“…Cumulative evidence from studies suggests the contribution of DR3 in in many immune processes and in various autoimmune diseases sharing pathogenic pathways similar to vitiligo. For example, elevated serum TL1A acts on mononuclear cells through increased DR3 expression on CD8+ lymphocytes and CD14+ lymphocytes in the peripheral blood of patients with psoriasis vulgaris.TL1A could act in synergy with IL-23 to stimulate PBMCs from patients with PV to produce IL-17 that has role in pathogenesis in psoriasis [16].…”

Role of TL1A in Inflammatory Autoimmune Diseases: A Comprehensive Review

“…TC, TT+TC genotypes of rs3810936, rs7848647 were negatively correlated with RA risk ( Table 2 ). The CC genotype of TL1A rs7848647 polymorphism contributes to elevated TL1A levels and is associated with antibody production (RF expression) ( 65 ). Expression of genes related to proliferation of RA-FLSs, including spectrin repeat-containing nuclear envelope 1 (SYNE1), Fc receptor-like 2 (FCRL2), PYD (pyrin domain)-containing 1 (PYDC1), cell division cycle 45 homolog (CDC45), signal transducer and activator of transcription 5B (STAT5B), and interferon regulatory factor 4 (IRF4), was increased after TL1A stimulation ( 70 ).…”