Expression of cytokines in chicken peripheral blood mononuclear cells after stimulation by probiotic bacteria and <i>Campylobacter jejuni in vitro</i>

et al. 2019

In this study, a total of 180 one-day-old male Arbor Acres broilers were randomly allotted to 5 groups with 6 replicates per group. Birds in negative control group were fed a corn-soybean mealbased diet, and birds in positive control group, MEF group, COSE group, MEF + COSE group were challenged with E. coli K88 and supplemented with 0, 1 × 10 10 cfu MEF/kg of diets, 500 mg COSE/ kg of diets or their combinations, respectively. Results showed that PC group had lower average body weight and greater E. coli counts in caecal contents than NC group. MEF and COSE significantly increased average body weight, serum IgA level, T-SOD activity compared with that of PC group. MEF and MIX group had less caecal E. coli counts than PC birds. The results indicated dietary supplementation of MEF and COSE had a positive moderating effect on E. coli K88-challenged broiler chickens.

Section: Discussionmentioning

confidence: 60%

Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler chickens

Dong

et al. 2019

“…Previous studies have shown that C. jejuni increase the expression of a number of cytokines with chemoattractant and pro-inflammatory functions in human intestinal epithelial cells (Bahrami et al, 2011;Borrmann et al, 2007;Stašová et al, 2015). We investigated the production of pro-inflammatory cytokines (IL-6, IL-8, and MCP-1) released from human intestinal epithelial cells (Caco-2 and HT-29) in response to C. jejuni stimuli.…”

Section: Resultsmentioning

confidence: 99%

Grape seed extract (GSE) modulates campylobacter pro-inflammatory response in human intestinal epithelial cell lines

Silván

Mingo

Martínez-Rodríguez

2017

The main aim of this study was to investigate the effect of Grape seed extract (GSE) on Campylobacter induced-cytokine production by human intestinal cell lines. With this purpose, Caco-2 and HT-29 cells were challenged with culture supernatants from several strains of C. jejuni and levels of secreted IL-6, IL-8, and MCP-1 were measured. Cytokine production was higher in HT-29 than in Caco-2 cells, showing different levels of secretion depending of the epithelial cells origin. Also, infective isolates coming from campylobacteriosis patients rendered higher levels of proinflammatory cytokines. Co-treatment and pre-treatment of HT-29 cells with GSE and C. jejuni significantly reduced cytokines production in a dose-dependent manner. These results make this natural product a putative nutritional tool for use in the treatment of campylobacteriosis, which could contribute to improve disease prevention or reduce disease severity.

“…Moreover, the microorganisms used for CSM fermentation is focused on probiotics, such as Lactobacillus, B. subtilis, Yeast, or Fungi, which can positively affect the modulation either innate or acquired immunity, or both (Amerah et al, 2013;Bai et al, 2013). The live microbes in FCSM may act as probiotics to enhance humoral immune response of broiler (Latesh, Singh, & Manoj, 2013;Stašová et al, 2015). Besides, although the live microbes in the FCSM was dead through the drying process, heat-killed microbes also can generate beneficial biological responses to stimulate the gastrointestinal immune function (Adams, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers

Deng

et al. 2017

The study was to investigate the effects of fermented cottonseed meal (FCSM) on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers. The cottonseed meal (CSM) was fermented with Bacillus subtilis ST-141 and Saccharomycetes N5. Hundred and twenty-eight one-day-old male Arbor Acres broilers were randomly divided into four groups: a corn-soybean meal (SBM) basal diet, and three experimental diets (CSM, FCSM-1, and FCSM-2) in which the control diet was supplemented with CSM or FCSM to partially replace SBM. Results showed that the fermentation process can effectively decrease free gossypol level, and increase acid-soluble protein level in CSM. The levels of immunoglobulin, biochemical parameters, antioxidative abilities in serum or liver tissue, and Lactobacilli and total anaerobic bacteria counts in ceca digesta of birds fed FCSM-1 were improved compared with birds fed CSM or FCSM-2 on days 21 and 42. So FCSM can be effectively applied in broiler diets.