Expression of Cytokines and Inducible Nitric Oxide Synthase in Inflamed Gingival Tissue

Hirose, Michiko; Ishihara, Kazuyuki; Saitô, Atsushi; Nakagawa, Taneaki; Yamada, Satoru; Okuda, K

doi:10.1902/jop.2001.72.5.590

Cited by 130 publications

(138 citation statements)

References 43 publications

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“…iNOS activity was reported to increase in ligatureinduced periodontitis [27], experimental periodontitis [30], and chronic periodontitis [20,26,31]. Higher levels of iNOS expression were observed in chronic periodontitis tissues than in clinically healthy gingival tissues [20,23,26,27,31] and a previous study of ours showed that iNOS increased in the gingival tissue of aggressive periodontitis patients (unpublished data). In various animal models iNOS was susceptible to infections by a variety of pathogens [32][33][34][35].…”

Section: Introductionsupporting

confidence: 57%

“…NO has been implicated in the pathogenesis of apical infection [45], periapical granulomas [46], and inflamed human dental pulp [47]. Increased iNOS expression in gingiva was shown in periodontitis [20,22,26,28,31], ligature-induced periodontitis [27], experimental periodontitis [30,46], inflamed gingiva [23], localized aggressive periodontitis [21], and recently by our group in aggressive periodontitis (unpublished data).…”

Section: Discussionmentioning

confidence: 80%

“…It was reported that NOS activity plays a role in stimulating production of NO x . iNOS was observed in dental tissues, and increased iNOS production was reported in gingival crevicular fluid (GCF) [19] and gingival tissues [20][21][22][23] in different periodontal diseases, but iNOS activity was not observed in the gingival tissues in sterile animals [24]. Periodontal diseases are inflammatory disorders that cause tissue damage and loss via the complex interactions between pathogenic bacteria and host immune response.…”

Section: Introductionmentioning

confidence: 99%

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Nitric oxide in gingival crevicular fluid and nitric oxide synthase expression in the gingiva of patients with sickle cell disease

Güzeldemir

Toygar²,

Bal³

et al. 2011

Turk J Hematol

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Objective: This study aimed to biochemically measure the production of nitric oxide in gingival crevicular fluid and immunohistochemically measure the expression of inducible nitric oxide synthase in the gingiva of patients with sickle cell disease. Additionally, we aimed to obtain insight into the immunopathology of sickle cell disease by comparing inducible nitric oxide synthase levels in patients with sickle cell disease and controls using gingiva and gingival crevicular fluid. Materials and Methods: The study included 20 sickle cell disease patients and 20 healthy controls. Immunohistochemical analysis was used to measure inducible nitric oxide synthase expression in gingiva and nitric oxide levels in gingival crevicular fluid were spectrophotometrically measured. Results: Nitric oxide levels in the patients and controls did not differ significantly (21.2±4.5 and 23.1±2.3 μM L -1 , respectively, p>0.05). There weren't any statistically significant differences in infiltrated inflammatory cells, density of inflammatory cells that stained with inducible nitric oxide synthase, or nitric oxide expression in gingiva between the patient and control groups (p>0.05). Conclusion: To the best of our knowledge this is the first study to examine the expression of inducible nitric oxide synthase in the gingiva and gingival crevicular fluid in patients with sickle cell disease. Using the gingiva and gingival crevicular fluid we were unable to observe sickle cell disease-associated inducible nitric oxide synthase expression and a difference in nitric oxide levels.

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Section: Introductionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Nitric oxide in gingival crevicular fluid and nitric oxide synthase expression in the gingiva of patients with sickle cell disease

Güzeldemir

Toygar²,

Bal³

et al. 2011

Turk J Hematol

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“…6,21 IL-10 is expressed in both healthy and diseased human periodontal tissues and is reported to be reduced in patients infected with Aggregatibacter actinomycetemcomitans. 22 The gene encoding IL-10 was mapped to chromosome 1q31-32. The three SNPs(-1082 G/A; -819 C/T and -592 C/A) from the transcriptional start site have been associated with the altered synthesis of IL-10 in response to inflammatory stimuli.…”

Section: Introductionmentioning

confidence: 99%

IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters

et al. 2017

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“…The inducible isoform (iNOS) produces high quantities of NO when stimulated by many proinflammatory cytokines and is expressed in several types of inflammatory cells (Ricciardolo et al, 2004). The main neurotransmitters of excitatory NANC neurotransmitter are substance P (SP) and neurokinin A (NKA), which play significant roles in priming and recruiting of inflammatory cells and are also related to the pain and neurogenic inflammation presented in PD (Hirose et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The Expression of Nitric Oxide in the Gingival Tissue in Subjects with Periodontitis and Chronic Pain

Fabri¹,

Sannomiya²,

Prado

et al. 2014

Int. J. Odontostomat.

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Periodontal disease (PD) is a chronic infection that may have local and systemic rebound. Although a series of inflammatory mediators are involved in PD, the mechanisms involved in chronic craniofacial pain associated with it require elucidation. The aim of this study was to evaluate the immunoreactivity of substance P (SP), neuronal (nNOS) and inducible (iNOS) nitric oxide synthases in gingival samples of patients with severe PD with and without chronic craniofacial pain. Gingival specimens were obtained during routine periodontal surgery while managing 20 patients with both PD and chronic craniofacial pain (CFP Group) and 18 patients with only PD (PD Group). Following surgical removal, the tissue underwent routine histological techniques and was stained by immunohistochemistry with antibodies against SP, nNOS and iNOS. Using an image analysis system, we assessed the SP, nNOS and iNOS content in total gingival tissue as well as in both epithelial and connective gingival area. We observed high expression of nNOS in gingival tissue obtained from CFP patients (p<0.001), particularly in the epithelium area (p<0.001) comparatively to PD patients. In addition, the iNOS expression was also increased in the CFP group in the connective gingival tissue (p=0.003). There was no difference concerning SP expression between the groups. Our results suggest that nitric oxide, particularly derived from nNOS, modulates not only PD but also chronic craniofacial pain, since patients with this association presented an increase in nNOS and iNOS expression in gingival tissue.

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Expression of Cytokines and Inducible Nitric Oxide Synthase in Inflamed Gingival Tissue

Abstract: The expression of IL-6 may reflect inflammation in gingival tissue, and iNOS may be involved in the inflammatory process in periodontitis. The presence of A. actinomycetemcomitans or P. gingivalis might relate to the different cytokine profiles of IL-1alpha, IL-8, and IL-10.

Cited by 130 publications

References 43 publications

Nitric oxide in gingival crevicular fluid and nitric oxide synthase expression in the gingiva of patients with sickle cell disease

Nitric oxide in gingival crevicular fluid and nitric oxide synthase expression in the gingiva of patients with sickle cell disease

IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters

The Expression of Nitric Oxide in the Gingival Tissue in Subjects with Periodontitis and Chronic Pain

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