Expression of chimeric Bt gene, Cry1Aabc in transgenic pigeonpea (cv. Asha) confers resistance to gram pod borer (Helicoverpa armigera Hubner.)

Expression of a Chimeric Gene Encoding Insecticidal Crystal Protein Cry1Aabc of Bacillus thuringiensis in Chickpea (Cicer arietinum L.) Confers Resistance to Gram Pod Borer (Helicoverpa armigera Hubner.)

“…Protein expression studies using different plant tissues collected at different developmental stages of three chickpea events indicated expression variation as reported earlier in cotton ( Dong and Li, 2007 ), chickpea ( Acharjee et al, 2010 ), and pigeonpea ( Das et al, 2016 ). In leaf tissues, protein expression during pre-flowering stage was higher as compared to the post-flowering stage.…”

Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata)

“…Also, codon usage was optimized to enhance translation in dicotyledonous plants by using plant-preferred codons. Using a similar strategy, Das et al (2016) constructed a plant codon-optimized chimeric Cry1Aabc gene for expression in pigeon pea. Likewise, high level expressions of optimized cry1Ac, cry1Ab and cry1C genes have been reported for engineering resistance to lepidopteran insect pests in transgenic cotton, tomato and tobacco, respectively (Perlak et al 1990(Perlak et al , 1991van der Salm et al 1994).…”

Assessment of Pigeonpea (Cajanus cajan L.) transgenics expressing Bt ICPs, Cry2Aa and Cry1AcF under nethouse containment implicated an effective control against herbivory by Helicoverpa armigera (Hübner)

“…45,46 A major impetus for the mitigation of this pest in pigeonpea has been transgenesis due to the lack of availability of resistance sources in the cultivated germplasm. 47,48 Studies accruing from the literature, [14][15][16][17][18][19] including the work from our group, 15 have demonstrated the feasibility of curtailing this pest in pigeonpea using different Bt ICPs under laboratory conditions. However, there has not been a single report demonstrating the performance of these transgenic events under soil conditions.…”

“…The major emphasis of our group has been in the development of transgenics in pigeonpea for mitigation of the pod borer H. armigera using a tissue culture‐independent in planta transformation strategy . Several other research groups have also produced transgenic pigeonpea with the development of diverse Bt genes . The ability of transformed plants to perform against insect pressure might vary between laboratory and soil conditions.…”

“…[11][12][13][14][15] Several other research groups have also produced transgenic pigeonpea with the development of diverse Bt genes. [14][15][16][17][18][19] The ability of transformed plants to perform against insect pressure might vary between laboratory and soil conditions. It is therefore imperative that the plants be assessed for trait efficacy under soil conditions.…”