Expression of CD1d and Ligand-Induced Cytokine Production Are Tissue Specific in Mucosal Epithelia of the Human Lower Reproductive Tract

Kawana, Kei; Matsumoto, Junko; Miura, Shigeki; Shen, Li; Kawana, Yukiko; Nagamatsu, Takeshi; Yasugi, Toshiharu; Fujii, Tomoyuki; Yang, Huixia; Quayle, Alison J.; Taketani, Yuji; Schust, Danny J.

doi:10.1128/iai.01672-07

Cited by 40 publications

(59 citation statements)

References 37 publications

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“…Kawana et al showed different levels of CD1d expression in epithelial cells in the lower reproductive tract, ranging from high expression in the vagina, ectocervix and penile urethra and very low expression in endocervix and endometrium [31]. We found different results: low CD1d expression (ranging from 4% to 8%) in material obtained from patients with genital prolapse.…”

Section: Discussioncontrasting

confidence: 55%

Establishment and Characterization of Vaginal Tissue Primary Culture: Feasibility of Cell Therapy for Mayer-Rokitansky-Kuster-Hauser Syndrome (MRKHS) Patient Treatment

Invitti¹,

Cc²,

Pb³

et al. 2017

J Biomedical Sci

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Background:The uterine and vaginal agenesis, MayerRokitansky-Küster-Hauser Syndrome (SMRKH), is a congenital malformation that implies in the impairment of sexual activity and reproductive life. The first line therapy is nonsurgical (dilatation method) but surgical methods may be necessary to create a functional neovagina. In the surgical procedure, various types of graft materials have been used to line the neovagina, and recently, alternative grafts have been studied to minimize adverse effects. Our aim was to establish vaginal primary cultures in order to prove the feasibility of cell therapy for the reconstruction of the vaginal lining.

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Section: Discussioncontrasting

confidence: 55%

Establishment and Characterization of Vaginal Tissue Primary Culture: Feasibility of Cell Therapy for Mayer-Rokitansky-Kuster-Hauser Syndrome (MRKHS) Patient Treatment

Invitti¹,

Cc²,

Pb³

et al. 2017

J Biomedical Sci

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show abstract

“…In addition to the study (7) reported in the letter by Hu et al, showing reduced CD1d expression in cells expressing the HPV-E5 protein, the same group in an earlier study showed that weak CD1d expression in uninfected lower reproductive tract tissues (vagina, endocervix) could be induced to higher levels by exposure to IFN-g (8). Although we were unable to detect altered levels of CD1d expression in hyperproliferative keratinocytes of K14E7 transgenic mouse skin (unpublished data), we speculate that HPV proteins involved in the early course of infection, such as E5 protein, may initially cause downregulation of CD1d and potentially other MHC molecules in infected epithelial cells as a mechanism of viral immune escape.…”

mentioning

confidence: 72%

Response to Comment on “Invariant NKT Cells in Hyperplastic Skin Induced a Local Immune Suppressive Environment by IFN-γ Production”

Mattarollo

Frazer

2012

The Journal of Immunology

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“…We here extended the potential of vaginal immunization for induction of protective immunity in the female genital tract to a non TLR-targeting immunostimulator, namely ␣-GalCer. It is noteworthy that a recent report has shown that CD1d is expressed on the epithelial cells in the human vagina (30). However, additional studies are required to evaluate the applicability of vaginal immunization to humans, and to understand the molecular and cellular mechanisms involved in generation of immunity in the female genital tract.…”

Section: Discussionmentioning

confidence: 99%

The Mucosal Adjuvant Effect of α-Galactosylceramide for Induction of Protective Immunity to Sexually Transmitted Viral Infection

Lindqvist

Persson

Thörn

et al. 2009

The Journal of Immunology

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Development of mucosal adjuvants to generate immunity in the female genital tract may have important implications for the development of vaccines to counter sexually transmitted infections. α-Galactosylceramide (α-GalCer) is presented by CD1d molecule on APCs to invariant Vα14+ NKT (iNKT) cells, which upon activation rapidly produce large amounts of immunomodulatory cytokines, leading to activation of a variety of innate and adaptive immune cells. Here, we assessed whether α-GalCer could act as a mucosal adjuvant for induction of protective immunity against genital herpes. We found that intranasal immunization with HSV-2 glycoprotein D (gD) in combination with α-GalCer elicits strong systemic gD-specific IgG Ab response as well as lymphoproliferative response with a mixed Th1/Th2 cytokine profile in the spleen, mediastinal lymph nodes, and genital lymph nodes. Importantly, such an immunization scheme conferred complete protection against an otherwise lethal vaginal HSV-2 challenge. We could also show that intravaginal immunization with gD plus α-GalCer generates potent gD-specific lymphoproliferative and IFN-γ responses in the genital lymph nodes and spleen. Furthermore, the vaginally immunized mice developed a strong systemic and mucosal IgG Ab response and protection against vaginal HSV-2 challenge. The mucosal adjuvant effect of α-GalCer was found to be mediated via CD1d molecule and appeared to be independent of the usage of the adaptor molecule MyD88. To our knowledge, this is the first report on the mucosal adjuvant effect of α-GalCer for induction of protective immunity against a sexually transmitted pathogen.

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Expression of CD1d and Ligand-Induced Cytokine Production Are Tissue Specific in Mucosal Epithelia of the Human Lower Reproductive Tract

Cited by 40 publications

References 37 publications

Establishment and Characterization of Vaginal Tissue Primary Culture: Feasibility of Cell Therapy for Mayer-Rokitansky-Kuster-Hauser Syndrome (MRKHS) Patient Treatment

Establishment and Characterization of Vaginal Tissue Primary Culture: Feasibility of Cell Therapy for Mayer-Rokitansky-Kuster-Hauser Syndrome (MRKHS) Patient Treatment

Response to Comment on “Invariant NKT Cells in Hyperplastic Skin Induced a Local Immune Suppressive Environment by IFN-γ Production”

The Mucosal Adjuvant Effect of α-Galactosylceramide for Induction of Protective Immunity to Sexually Transmitted Viral Infection

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