Expression of CD147, BIGH3 and Stathmin and their potential role as diagnostic marker in patients with urothelial carcinoma of the bladder

Bhagirath, Divya; Abrol, Nitin; Khan, Rehan; Sharma, Manoj; Seth, Amlesh; Sharma, Alpana

doi:10.1016/j.cca.2012.05.005

Cited by 46 publications

(36 citation statements)

References 26 publications

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“…A three-protein panel composed of PPARG, STMN1, and CAV2 has been shown to be associated with time to recurrence of bladder tumor (41). Bhagirath et al reported that the serum and urinary concentrations of STMN1 were higher in urothelial carcinoma of the bladder than in controls (36). These observations, taken together with the results of our current tissue proteome study, again support the idea that measuring dysregulated tissue proteins in human bodily fluids is a feasible strategy for discovering potentially useful noninvasive biomarkers.…”

Section: Comparison Of Tissue and Urine Proteomes In Bladder Cansupporting

confidence: 77%

Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer

Chen

Chung

et al. 2015

Molecular & Cellular Proteomics

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More than 380,000 new cases of bladder cancer are diagnosed worldwide, accounting for ϳ150,200 deaths each year. To discover potential biomarkers of bladder cancer, we employed a strategy combining laser microdissection, isobaric tags for relative and absolute quantitation labeling, and liquid chromatography-tandem MS (LC-MS/MS) analysis to profile proteomic changes in fresh-frozen bladder tumor specimens. Cellular proteins from four pairs of surgically resected primary bladder cancer tumor and adjacent nontumorous tissue were extracted for use in two batches of isobaric tags for relative and absolute quantitation experiments, which identified a total of 3220 proteins. A DAVID (database for annotation, visualization and integrated discovery) analysis of dysregulated proteins revealed that the three top-ranking biological processes were extracellular matrix organization, extracellular structure organization, and oxidation-reduction. Biological processes including response to organic substances, response to metal ions, and response to inorganic substances were highlighted by up-expressed proteins in bladder cancer. Seven differentially expressed proteins were selected as potential bladder cancer biomarkers for further verification. Immunohistochemical analyses showed significantly elevated levels of three proteins-SLC3A2, STMN1, and TAGLN2-in tumor cells compared with noncancerous bladder epithelial cells, and suggested that TAGLN2 could be a useful tumor tissue marker for diagnosis (AUC ‫؍‬ 0.999) and evaluating lymph node metastasis in bladder cancer patients. ELISA results revealed significantly increased urinary levels of both STMN1 and TAGLN2 in bladder cancer subgroups compared with control groups. In comparisons with agematched hernia urine specimens, urinary TAGLN2 in bladder cancer samples showed the largest fold change (7.13-fold), with an area-under-the-curve value of 0.70 (p < 0.001, n ‫؍‬ 205). Overall, TAGLN2 showed the most significant overexpression in individual bladder cancer tissues and urine specimens, and thus represents a potential biomarker for noninvasive screening for bladder cancer. Our findings highlight the value of bladder tissue proteome in providing valuable information for future validation studies of potential biomarkers in urothelial carcinoma. Molecular & Cellular Proteomics

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Section: Comparison Of Tissue and Urine Proteomes In Bladder Cansupporting

confidence: 77%

Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer

Chen

Chung

et al. 2015

Molecular & Cellular Proteomics

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“…25 The same features have been attributed to EMMPRIN. 26 The intriguing fact in our investigation was that, in none of those markers, the relations between serum and urine levels were preserved, suggesting that the latter may be considered a tissue-specific production within the kidney.…”

Section: Discussionmentioning

confidence: 91%

Urine matrix metalloproteinases and their extracellular inducer EMMPRIN in children with chronic kidney disease

2015

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Background: Transforming growth factor (TGF)beta1 and matrix metalloproteinases (MMPs) play an essential role in CKD-related tissue remodeling. However, there are no data on urine MMPs and their extracellular inducer EMMPRIN in CKD patients. The aim of study was to assess the concentrations of MMP-2, MMP-7, MMP-9, EMMPRIN and TGFbeta1 in serum and urine of CKD children and to analyze the potential relations between those parameters. Methods: Forty-one pre-dialysis CKD children and 23 age-matched controls were enrolled in the study. The concentrations of analyzed parameters were assessed by ELISA. Results: Serum and urine values of MMP-2, MMP-7, MMP-9, EMMPRIN and TGFbeta1 were significantly elevated in CKD patients versus controls. The MMP-2 and MMP-9 levels in urine correlated significantly with the corresponding values in serum, whereas MMP-7, EMMPRIN and TGFbeta1 urine concentrations did not. There were also significant correlations between urine values of all parameters. Conclusions: The increased urine levels of MMPs, EMMPRIN and TGFbeta1 indicate enhanced proteolysis and renal tissue remodeling. In the case of MMP-7, EMMPRIN and TGFbeta1 those disturbances seem independent of enhanced serum activity of the corresponding enzymes. The urine MMP-7 and EMMPRIN concentrations may serve as new independent indices of tissue remodeling and renal interstitial fibrosis in children with CKD.

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“…Indeed, CD147 has been suggested as a prognostic marker in endometrial cancer [65], gastric cancer [96], glioblastoma [98], HCC [78], lupus nephritis [130], UCB [94] and other diseases. Patients with high CD147 expression have a poor prognosis [104], while multivariate analysis revealed that the expression of CD147 is an independent prognostic factor for patients with NSCLC [81,176].…”

Section: Clinical Applications Of Cd147mentioning

confidence: 99%

The Biological Function and Clinical Utilization of CD147 in Human Diseases: A Review of the Current Scientific Literature

Xiong

Edwards

Zhou

2014

IJMS

186

194

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CD147 or EMMPRIN is a member of the immunoglobulin superfamily in humans. It is widely expressed in human tumors and plays a central role in the progression of many cancers by stimulating the secretion of matrix metalloproteinases (MMPs) and cytokines. CD147 regulates cell proliferation, apoptosis, and tumor cell migration, metastasis and differentiation, especially under hypoxic conditions. CD147 is also important to many organ systems. This review will provide a detailed overview of the discovery, characterization, molecular structure, diverse biological functions and regulatory mechanisms of CD147 in human physiological and pathological processes. In particular, recent studies have demonstrated the potential application of CD147 not only as a phenotypic marker of activated regulatory T cells but also as a potential diagnostic marker for early-stage disease. Moreover, CD147 is recognized as an effective therapeutic target for hepatocellular carcinoma (HCC) and other cancers, and exciting clinical progress has been made in HCC treatment using CD147-directed monoclonal antibodies.

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Expression of CD147, BIGH3 and Stathmin and their potential role as diagnostic marker in patients with urothelial carcinoma of the bladder

Cited by 46 publications

References 26 publications

Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer

Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer

Urine matrix metalloproteinases and their extracellular inducer EMMPRIN in children with chronic kidney disease

The Biological Function and Clinical Utilization of CD147 in Human Diseases: A Review of the Current Scientific Literature

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