Expression of CD10 by Human T Cells That Undergo Apoptosis Both In Vitro and In Vivo

Abstract

This study shows that human postthymic T cells express CD10 when undergoing apoptosis, irrespective of the signal responsible for initiating the apoptotic process. Cells from continuous T-cell lines did not normally express CD10, but became CD10+ when induced into apoptosis by human immunodeficiency virus (HIV) infection and exposure to CD95 monoclonal antibody, etoposide, or staurosporin. Inhibitors of caspases blocked apoptosis and CD10 expression. Both CD4+ and CD8+ T cells purified from normal peripheral blood expressed CD10 on apoptotic induction. CD10 was newly synthesized by the apoptosing cells because its expression was inhibited by exposure to cycloheximide and CD10 mRNA became detectable by reverse transcription-polymerase chain reaction in T cells cultured under conditions favoring apoptosis. To show CD10 on T cells apoptosing in vivo, lymph node and peripheral blood T cells from HIV+ subjects were used. These suspensions were composed of a substantial, although variable, proportion of apoptosing T cells that consistently expressed CD10. In contrast, CD10+ as well as spontaneously apoptosing T cells were virtually absent in peripheral blood from normal individuals. Collectively, these observations indicate that CD10 may represent a reliable marker for identifying and isolating apoptosing T cells in vitro and ex vivo and possibly suggest novel functions for surface CD10 in the apoptotic process of lymphoid cells.

Angioimmunoblastic T‐cell lymphoma

“…In lymphocytes, its expression is largely restricted to B‐cell compartments with a typically high rate of apoptosis (Chu et al, 2000). CD10 is not expressed by normal peripheral T cells or nodal peripheral T‐cell lymphomas, but is induced in T cells undergoing apoptosis during HIV infection in both in vivo and in vitro experiments (Cutrona et al , 1999). This close association with CD10 expression and apoptosis suggests that CD10 plays a role in the regulation of lymphocyte survival.…”

CD10 expression in stromal cells of oral cavity squamous cell carcinoma: a clinic and pathologic correlation

“…CD10 has also been shown to be present in a variety of neoplasms such as renal cell carcinoma, prostatic adenocarcinoma, transitional cell carcinoma, endometrial stromal sarcoma, pancreatic adenocarcinoma, rhabdomyosarcoma, malignant melanoma, schwannoma, endometrial stromal tumors, intraductal breast papilloma, ductal breast hyperplasia, breast fibroadenoma and phyllodes tumor (Xu et al, 2001(Xu et al, , 2002Moritani et al, 2002;Oliva et al, 2002). A possible role of CD10 in the identification and isolation of apoptosing T cells in vitro and ex vivo (Cutrona et al, 1999) and in the growth of androgen-independent prostate cancer has been suggested (Albrecht et al, 2002). CD10 has also been shown to play an important role in the progression of malignant melanoma (Bilalovic et al, 2004).…”

Expression of CD10 as a prognostic and predictive factor in urothelial carcinomas

“…In particular, these authors suggest that CD10 is capable to enhance cancer cells invasion and metastasis. A number of other studies also discuss CD10 regulating biological activities of peptide substanses by reducing the concentration for receptor binding and signal transduction (18,19,23). Having in mind these contradictory data, we believe that our results provide convinsing evidense at least for CD10 role in urothelial cancer invasiveness in underlying smooth muscle.…”