Expression of caspase activation recruitment and pyrin domain levels of apoptosis-associated speck-like protein complex in the pancreas of rats subjected to experimental pancreatitis

Abstract

<jats:p> The present study investigated the effect of rutin, a natural flavonoid, on the expression of caspase activation recruitment domain (CARD) and pyrin domain (PYD) of apoptosis-associated speck-like protein (ASC), a mediator of inflammation, in the pancreas of rats administered with ethanol (EtOH) and high-fat diet (HFD). Pancreatitis was induced in male albino Wistar rats by administering EtOH (8–12 g/kg/day) and HFD (22% fat) for 90 days. In addition, rats also received 100 mg rutin/kg body weight orally from 31st day till the experimental period. Serum levels of cytokines, interleukin 18 (IL-18) and IL-6; activity levels of caspase-1 and myeloperoxidase (MPO); messenger RNA (mRNA) expression of tumor necrosis factor α (TNF-α), caspase-1, CARD and PYD of ASC; and histological changes in pancreas were assessed. We observed a significant elevation in serum IL-18, IL-6, caspase-1 and MPO activities, mRNA expression of PYD, TNF-α and caspase-1 in the pancreas of rats administered with EtOH and HFD. Rutin administration along with EtOH and HFD significantly upregulated the mRNA expression of CARD and downregulated PYD, caspase-1, and TNF-α expressions. Rutin supplementation was also found to reduce IL-18 and IL-6 levels; and inflammatory changes in tissue architecture were evidenced by histological observations. The anti-inflammatory activity of rutin might be due to its effect on modulating the expression of ASC complex that mediates inflammation. </jats:p>

Anti-cancer and antioxidant properties of phenolics isolated from<i> Toona sinensis</i> A Juss acetone leaf extract

“…Rutin is a kind of flavanol glycoside, richly present in black tea, apple skin peels and buckwheat. It has been reported that rutin possesses antioxidant properties, scavenges hydroxyl and superoxide radicals and inhibits lipid peroxidation [26]. Thus the presence of quercitrin and rutin indicate that TS leaf has significant nutrition and health benefits.…”

Can rutin ameliorate aluminum phosphide-induced acute cardiac toxicity in adult albino rats?

“…These findings were supported by Lv et al (2018) who demonstrated that treatment with rutin significantly reversed the induced reductions of SOD, CAT, GST and GSH-Px activities in chronic heart disease in the pig. In addition, it has been explained that rutin has an anti-inflammatory activity due to modulation of apoptosis-associated proteins (Aruna et al, 2013). In this regard, Hsieh et al (2014) explained that the treatment of isolated hearts with green tea extract rich in flavonoids as rutin showed marked protection of hearts from oxidative stress and avoid cell edema and apoptosis.…”

Phytoceuticals in Acute Pancreatitis: Targeting the Balance between Apoptosis and Necrosis

“…Inflammation. Rutin tempered inflammation (lower CRP) and protected pancreas against inflammatory injury in both acute and chronic pancreatitis models via several mechanisms: decreased expression of cytokines (IL-1 β , IL-6, and TNF- α ) and decreased neutrophil infiltration (measured as MPO activity) [ 24 , 132 – 134 ]. Rutin supplementation also preserved pancreatic microarchitecture, improved food consumption, and maintained net weight gain in rats with alcohol-induced and high-fat-induced chronic pancreatitis [ 24 ].…”