Expression of cardiac function genes in adult stem cells is increased by treatment with nitric oxide agents

Rebelatto, Carmen K.; Aguiar, Alessandra Melo de; Senegaglia, Alexandra Cristina; Aita, Carlos Alberto Mayora; Hansen, Paula; Barchiki, Fabiane; Kuligovski, Crisciele; Olandoski, Márcia; Moutinho, J.; Dallagiovanna, Bruno; Goldenberg, Samuel; Brofman, Paulo; Nakao, Lia S.; Correa, Alejandro

doi:10.1016/j.bbrc.2008.11.061

Cited by 21 publications

(17 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the RNAs associated with polysomes represent the cellular phenotype more faithfully than analyses of total mRNA; unfortunately, total mRNA is still widely used in most stem cell studies. Moreover, it has been observed in ADSCs differentiation assays that control cultures without differentiation inducers express several genes at the mRNA level, specific for diverse lineages such as cardiomyocytes, osteoblasts, adipocytes, and condrocytes [10,33]. In addition, promiscuous gene expression has been suggested or shown by others in MSCs, including ADSCs [13], and also in other stem cell populations [12,34,35]; these observations clearly indicate that total mRNA populations do not provide a good molecular representation of ADSCs.…”

Section: Discussionmentioning

confidence: 95%

Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts

Zych

Spangenberg²,

Stimamiglio³

et al. 2014

Stem Cells and Development

Self Cite

View full text Add to dashboard Cite

Although fibroblasts and multipotent stromal/stem cells, including adipose-derived stromal cells (ADSCs), have been extensively studied, they cannot be clearly distinguished from each other. We, therefore, investigated the cellular and molecular characteristics of ADSCs and fibroblasts. ADSCs and fibroblasts share several morphological similarities and surface markers, but were clearly found to be different types of cells. Contrary to previous reports, fibroblasts were not able to differentiate into adipocytes, osteoblasts, or chondrocytes. Polysome-bound mRNA profiling revealed that *1,547 genes were differentially expressed (DE) in the two cell types; the genes were related to cell adhesion, the extracellular matrix, differentiation, and proliferation. These findings were confirmed by functional analyses showing that ADSCs had a greater adhesion capacity than fibroblasts; the proliferation rate of fibroblasts was also higher than that of ADSCs. Importantly, 185 DE genes were integral to the plasma membrane and, thus, candidate markers for ADSC isolation and manipulation. We also observed that an established marker of fibroblasts and ADSCs, CD105, was overexpressed in ADSCs at both mRNA and protein levels. CD105 expression seemed to be related to differentiation capacity, at least for adipogenesis. This study shows that ADSCs and fibroblasts are distinct cell types. These findings should be taken into account when using these two cell types in basic and therapeutic studies.

show abstract

Section: Discussionmentioning

confidence: 95%

Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts

Zych

Spangenberg²,

Stimamiglio³

et al. 2014

Stem Cells and Development

Self Cite

View full text Add to dashboard Cite

show abstract

“…The use of 5-azacytidine (5-aza) is effective for cardiomyocyte differentiation, but it is clinically toxic [2]. Several other methods have been used to promote the differentiation of stem cells into cardiomyocytes, including the coculture of stem cells [3], treatment with cardiac tissue extracts [4], angiotensin II [5], and nitric oxide donors [6, 7]. Most of these methods are inefficient, generating only a small percentage of differentiated cells.…”

Section: Introductionmentioning

confidence: 99%

A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells

Gao

Guo

Jiang

et al. 2014

Stem Cells International

View full text Add to dashboard Cite

A growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was designed: we investigated the role of 5-azacytidine (5-aza), salvianolic acid B (SalB), and cardiomyocyte lysis medium (CLM) in inducing MSCs to acquire the phenotypical characteristics of cardiomyocytes. The fourth-passage MSCs were treated with 5-aza, SalB, CLM, 5-aza+salB, 5-aza+CLM, SalB+CLM, and 5-aza+SalB+CLM for 2 weeks. Immunofluorescence results showed that cTnT expression in the 5-aza+salB+CLM group was stronger than other groups. Real-time qPCR and Western blotting analyses showed that cTnT, alpha-cardiac actin, mef-2c, Cx43, and GSK-3beta expression increased while beta-catenin expression decreased. The salB+5-aza+CLM group had the most evident effects. SalB combined with 5-aza and CLM improved cardiomyocyte differentiation from MSCs. In the MSCs differentiation process, the Wnt/beta-catenin signaling pathway had been inhibited.

show abstract

“…RT-PCR and quantitative RT-PCR were performed as described previously [32]. For a list of the primers used, see supporting Additional file 8: Table S2.…”

Section: Methodsmentioning

confidence: 99%

Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules

et al. 2014

Self Cite

View full text Add to dashboard Cite

BackgroundDZIP1 (DAZ-interacting protein 1) has been described as a component of the Hh signaling pathway with a putative regulatory role in ciliogenesis. DZIP1 interacts with DAZ RNA binding proteins in embryonic stem cells and human germ cells suggesting a role in mRNA regulation.ResultsWe investigated DZIP1 function in HeLa cells and its involvement in ribonucleoprotein complexes. DZIP1 was predominantly located in granules in the cytoplasm. Under oxidative stress conditions, DZIP1 re-localized to stress granules. DZIP appears to be important for the formation of stress granules during the stress response. We used immunoprecipitation assays with antibodies against DZIP1 and microarray hybridization to identify mRNAs associated with DZIP1. The genetic networks formed by the DZIP1-associated mRNAs were involved in cell cycle and gene expression regulation. DZIP1 is involved in the Hedgehog signaling pathway. We used cyclopamine, a specific inhibitor of this pathway, to analyze the expression of DZIP1 and its associated mRNAs. The abundance of DZIP1-associated mRNAs increased with treatment; however, the silencing or overexpression of DZIP1 in HeLa cells had no effect on the accumulation of the associated mRNAs. Polysomal profile analysis by sucrose gradient centrifugation demonstrated the presence of DZIP1 in the polysomal fraction.ConclusionsOur results suggest that DZIP1 is part of an RNP complex that occupies various subcellular locations. The diversity of the mRNAs associated with DZIP1 suggests that this protein is a component of different RNPs associated with translating polysomes and with RNA granules.

show abstract

Expression of cardiac function genes in adult stem cells is increased by treatment with nitric oxide agents

Cited by 21 publications

References 40 publications

Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts

Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts

A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells

Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules

Contact Info

Product

Resources

About