Expression of autocrine motility factor receptor in human esophageal squamous cell carcinoma

Abstract

Autocrine motility factor and its receptor (gp78) have been shown to play an important role in tumor cell migration, invasion and metastasis. We have detected gp78 expression in buffered-formalin-fixed, paraffin-embedded sections of esophageal squamous cell carcinomas using an anti-gp78 monoclonal antibody (MAb), 3F3A, and examined the relationship between gp78 expression and clinicopathological and prognostic factors. In 55 of 101 (54%) patients, gp78 was detected in the tumor cells. The frequency of gp78-positive expression was significantly associated with tumor size, infiltrative growth, depth of invasion and lymph node metastasis. The cumulative survival rate of patients with gp78 was significantly lower than that of patients without gp78. Our results suggest that autocrine motility factor receptor (gp78) expression could be a useful biomarker for malignancy grading and prognosis in patients with esophageal squamous cell carcinoma.

Immunohistochemical expression of Type IV Collagen and Autocrine Motility Factor Receptor in Odontogenic Tumours

“…Nakamori S et al, [17] and Maruyama et al, [18] also reported absence of significant expression of AMFR in normal tissues Thus, generally AMFR is shown to be minimally expressed in normal tissues.…”

Expression of autocrine motility factor receptor correlates with disease progression in human gastric cancer

“…After rehydration and washing with phosphatebuffered saline (PBS, pH 7.2), sections were incubated with 10% normal goat serum (Dako, Glostrup, Denmark) in PBS for 20 min at room temperature to block non-specific binding of the second antibody. After blocking against endogenous avidin and biotin, sections were incubated with anti-gp78 monoclonal antibody, 3F3A (Nabi et al, 1990;Silletti et al, 1991;Nakamori et al, 1994;Otto et al, 1994;Maruyama et al, 1995) (Cox, 1972). The difference was considered to be significant when P was less than 0.05.…”

“…(Dako, Glostrup, Denmark) in PBS for 20 min at room temperature to block non-specific binding of the second antibody. After blocking against endogenous avidin and biotin, sections were incubated with anti-gp78 monoclonal antibody, 3F3A (Nabi et al, 1990;Silletti et al, 1991;Nakamori et al, 1994;Otto et al, 1994;Maruyama et al, 1995), at a dilution of 1:200 in PBS containing 1% bovine serum albumin (Sigma, St. Louis, MO, USA) overnight at 4°C. After three rinses in PBS, sections were incubated with biotinylated anti-rat immunoglobulin (Dako) for 1 h at room temperature, washed three times with PBS and reacted with streptoavidin-biotin system Correspondence: Y Hirono, Department of Surgery, Keiju General Hospital, Tomioka-cho 94, Nanao, Ishikawa, 926, Japan Received 5 January 1996; revised 7 June 1996; accepted 1 July 1996 (Dako), using 0.04% 3,3'-diaminobenzide tetrahydrochloride (Sigma) for 1 min as chromogen.…”

“…The degree of monoclonal antibody reactivity with each tissue sections was considered positive if unequivocal staining of membrane or cytoplasm was seen in more than 10% of tumour cells as described previously (Otto et al, 1994;Maruyama et al, 1995). All sections were analysed blind without knowledge of the patient's treatment outcome or clinicopathological findings.…”

“…Expression of this receptor relates to cell motility-regulating effects and also may play an important role in tumour cell invasion or metastasis (Nabi et al, 1992;Watanabe et al, 1991b). Recent studies have demonstrated that increased expression of gp78 is correlated with a high incidence of recurrence and decreased survival of patients with colorectal cancer, bladder cancer and oesophageal cancer (Nakamori et al, 1994;Otto et al, 1994;Maruyama et al, 1995).…”