Expression of Antiviral Activity and Induction of 2′,5′-Oligoadenylate Synthetase by Conceptus Secretory Proteins Enriched in Bovine Trophoblast Protein-11

Short, Everett C.; Geisert, R. D.; Helmer, Stephen D.; Zavy, M. T.; Rw, Fulton

doi:10.1095/biolreprod44.2.261

Cited by 46 publications

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“…Conceptus length and antiviral activity in the uterus (a measure of IFNT2 bioactivity; Alexenko et al1997) were highly variable, as is typical at this stage of pregnancy (Short et al 1991, Mann & Lamming 2001, Block et al 2007), but the largest conceptuses were in the CSF2 group. Antiviral activity tended to be greater for cows receiving CSF2 conceptuses and IFNT2 gene expression was 22 times greater for CSF2 conceptuses.…”

Section: Discussionmentioning

confidence: 99%

Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression

Loureiro¹,

Block

Favoreto

et al. 2011

Reproduction

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Exposure of bovine conceptuses to colony-stimulating factor 2 (CSF2) from days 5 to 7 of development can increase the percentage of transferred conceptuses that develop to term. The purpose of this experiment was to understand the mechanism by which CSF2 increases embryonic and fetal survival. Conceptuses were produced in vitro in the presence or absence of 10 ng/ml CSF2 from days 5 to 7 after insemination, transferred into cows, and flushed from the uterus at day 15 of pregnancy. There was a tendency (PZ0.07) for the proportion of cows with a recovered conceptus to be greater for those receiving a CSF2-treated conceptus (35% for control versus 66% for CSF2). Antiviral activity in uterine flushings, a measure of the amount of interferon-t (IFNT2) secreted by the conceptus, tended to be greater for cows receiving CSF2-treated conceptuses than for cows receiving control conceptuses. This difference approached significance when only cows with detectable antiviral activity were considered (PZ0.07). In addition, CSF2 increased mRNA for IFNT2 (PZ0.08) and keratin 18 (P!0.05) in extraembryonic membranes. Among a subset of filamentous conceptuses that were analyzed by microarray hybridization, there was no effect of CSF2 on gene expression in the embryonic disc or extraembryonic membranes. Results suggest that the increase in calving rate caused by CSF2 treatment involves, in part, more extensive development of extraembryonic membranes and capacity of the conceptus to secrete IFNT2 at day 15 of pregnancy.

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Section: Discussionmentioning

confidence: 99%

Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression

Loureiro¹,

Block

Favoreto

et al. 2011

Reproduction

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“…The OAS1 expression was amplified during days 15-18 in heifers 36 and cow 37 . Shirasuna et al (2012) documented that, the OAS1 expression did not vary in pregnant and nonpregnant cows 25 .…”

Section: '-5' Oligoadenylate Synthetase (Oas1)mentioning

confidence: 99%

Interferon Stimulated Genes (Isgs): Novel Pregnancy Specific Biomarker In Buffaloes (Bubalus bubalis

Sarkar¹

2018

J Immunological Sci

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Early embryonic mortality (EEM) has been shown to be the prime cause of pregnancy failure in domestic species incurring severe economic losses in terms of milk production in dairy cows in most of the tropical countries including India. Despite of the availability of various diagnosis methods for the pregnancy detection the domestic animals are still prone to reproductive failure before the onset of implantation. Recently, a group of genes called as interferon stimulated genes (ISG) have been shown to be expressed during peri-implanation period which could serve as a potential diagnostic marker for early detection of pregnancy in domestic species. The present mini review highlights the differential expression dynamics of interferon stimulated genes (ISG) during early pregnancy period in buffalo.

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“…Testing this hypothesis revealed that pregnancy and IFNT induced the production of uncharacterised proteins by the endometrium [17]. Some of these proteins and genes induced by pregnancy/IFNT were later revealed to be classical Type I IFN stimulated genes (ISGs) 2',5'-oligoadenylate synthetase 1, 40/46 kDa; OAS1 [18,19] With the widespread availability of large-scale transcriptomic analysis, the molecular mechanisms required to establish uterine receptivity to implantation and pregnancy recognition are beginning to be elucidated. The initial in vivo analysis of the effect of the conceptus on endometrial gene expression was carried out using subtracted cDNA libraries [28] and a model of monozygotic twins to reduce genetic variation.…”

mentioning

confidence: 99%

Transcriptomic Analysis of the Bovine Endometrium: What is Required to Establish Uterine Receptivity to Implantation in Cattle ?

2012

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Abstract. In cattle, the majority of pregnancy loss can be attributed to early embryonic loss which occurs prior maternal recognition of pregnancy on Day 16 (Day 0 = ovulation). During this time, carefully orchestrated spatio-temporal alterations in the transcriptomic profile of the endometrium are required to drive conceptus elongation, via secretions from the endometrium (termed histotroph) and establish uterine receptivity to implantation. The two main modulators of these processed are progesterone (P4) and the pregnancy recognition signal interferon tau (IFNT). Altered concentrations of P4 in circulation mediate its effects via the endometrium and have been associated with different rates of conceptus elongation in cattle. Transcriptomic analysis of the endometrium has shown that modulation of circulating P4 alters endometrial expression of genes that can contribute to histotroph composition, which is beneficial (when P4 is supplemented) or detrimental (when P4 is reduced) to the developing conceptus. In addition, down-regulation of the progesterone receptor, required to establish uterine receptivity, is altered in the endometrium of heifers with altered P4 concentrations. IFNT, a type 1 interferon, also significantly impacts on the endometrial transcriptome. It induces the expression of a large number of classical interferon stimulated genes as early as Day 15 of pregnancy. In summary, the successful establishment of pregnancy in cattle requires a sequence of key events to ensure appropriate maternally derived secretions, establish uterine receptivity to implantation as well as an adequate endometrial response to IFNT production. Key words: Estrous cycle, Gene expression, Pregnancy, Progesterone (J. Reprod. Dev. 58: [189][190][191][192][193][194][195] 2012) I n cattle, as in other ungulate ruminants, the implantation process is characterized by a protracted period of elongation of the hatched blastocyst followed by apposition, attachment and adhesion of the trophectoderm to the uterine luminal epithelium (LE) [1,2]. Following successful fertilisation of an oocyte in the oviduct, the resulting embryo enters the uterus on approximately day 4 of pregnancy where it undergoes a number of cell divisions to form the morula which, after further differentiation, forms a blastocyst consisting of the inner cells mass (which will eventually give rise to the embryo/foetus) and an outer cell mass consisting of trophectoderm cells which ultimately give rise to the placenta. Up to the blastocyst stage of development, the embryo is relatively autonomous i.e. contact with the maternal uterine environment is not strictly required, as evidenced by the fact it is possible to culture embryos in vitro up to the blastocyst stage of development. However, once the embryo hatches from the zona pellucida (approximately day 9-10), significant morphological changes occur in the conceptus of domestic animals, including cattle, that differentiate the early pregnancy events from those of rodents, humans and non-human primates. The hatch...

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Expression of Antiviral Activity and Induction of 2′,5′-Oligoadenylate Synthetase by Conceptus Secretory Proteins Enriched in Bovine Trophoblast Protein-11

Cited by 46 publications

References 0 publications

Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression

Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression

Interferon Stimulated Genes (Isgs): Novel Pregnancy Specific Biomarker In Buffaloes (Bubalus bubalis

Transcriptomic Analysis of the Bovine Endometrium: What is Required to Establish Uterine Receptivity to Implantation in Cattle ?

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