Expression of an Arabidopsis lectin kinase receptor gene, lecRK-a1, is induced during senescence, wounding and in response to oligogalacturonic acids

Riou, Chantal; Hervé, Christine; Pacquit, Valérie; Dabos, Patrick; Lescure, Bernard

doi:10.1016/s0981-9428(02)01390-6

Cited by 63 publications

(44 citation statements)

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“…Riou et al (2002) have shown that two Arabidopsis LecRK genes are regulated developmentally and have suggested that they play an important role in both the developmental program and adaptive processes (e.g. wounding) in this species.…”

Section: Discussionmentioning

confidence: 99%

“…5) suggests roles for these genes predominantly in this organ. Thus, they are predicted to have a different role than two LecRK genes studied in Arabidopsis that are expressed predominantly in aerial tissues (Hervé et al, 1996(Hervé et al, , 1999Riou et al, 2002). Interestingly, an LecRK gene from poplar is also predominantly expressed in roots (Nishiguchi et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The Arabidopsis genome contains at least 42 LecRK sequences that have been grouped into three classes (A-C) plus an additional nine sequences of related soluble lectins (Barre et al, 2002). Searches in plant databases reveal that LecRK genes are widespread in higher plants, but apart from Arabidopsis (Hervé et al, 1996, Riou et al, 2002, LecRK genes have been studied only in lombardy poplar (Populus nigra var italica; Nishiguchi et al, 2002). RLK genes with legume lectin-like domains are not found in the complete genome sequences of yeast (Saccharomyces cerevisiae) or human (Homo sapiens).…”

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confidence: 99%

“…To date, the receptors of very few of these molecules have been cloned. Although there are as yet no functional studies on the role of LecRKs, detailed studies on the regulation of one of the Arabidopsis genes, Ath.lecRK-a1 (coding for protein At3g59700 in the Arabidopsis Information Resource [TAIR] database; Garcia-Hernandez et al, 2002), suggests that it is involved in plant development and also adaptive processes such as wounding (Riou et al, 2002). The poplar gene (PnLPK) is also up-regulated by wounding, but in contrast to Ath.…”

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confidence: 99%

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Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

et al. 2003

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To study the role of LecRK (lectin-like receptor kinase) genes in the legume-rhizobia symbiosis, we have characterized the four Medicago truncatula Gaernt. LecRK genes that are most highly expressed in roots. Three of these genes, MtLecRK7;1, MtLecRK7;2, and MtLecRK7;3, encode proteins most closely related to the Class A LecRKs of Arabidopsis, whereas the protein encoded by the fourth gene, MtLecRK1;1, is most similar to a Class B Arabidopsis LecRK. All four genes show a strongly enhanced root expression, and detailed studies on MtLecRK1;1 and MtLecRK7;2 revealed that the levels of their mRNAs are increased by nitrogen starvation and transiently repressed after either rhizobial inoculation or addition of lipochitooligosaccharidic Nod factors. Studies of the MtLecRK1;1 and MtLecRK7;2 proteins, using green fluorescent protein fusions in transgenic M. truncatula roots, revealed that they are located in the plasma membrane and that their central transmembrane-spanning helix is required for correct sorting. Moreover, their lectin-like domains appear to be highly glycosylated. Of the four proteins, only MtLecRK1;1 shows a high conservation of key residues implicated in monosaccharide binding, and molecular modeling revealed that this protein may be capable of interacting with Nod factors. However, no increase in Nod factor binding was found in roots overexpressing a fusion in which the kinase domain of this protein had been replaced with green fluorescent protein. Roots expressing this fusion protein however showed an increase in nodule number, suggesting that expression of MtLecRK1;1 influences nodulation. The potential role of LecRKs in the legumerhizobia symbiosis is discussed.The lectin-like receptor kinases (LecRKs) are a class of proteins originally described from Arabidopsis (Hervé et al., 1996). They have a structure similar to other plant receptor-like kinases (RLKs; Shiu and Bleecker, 2001;Cock et al., 2002) with an N-terminal targeting signal, a presumably extracellular domain, a single transmembrane (TM)-spanning helix, and a cytosolic kinase domain. The Arabidopsis genome contains over 610 RLKs that have been shown to be monophylogenetic with respect to the kinase domain and most closely related to the fruitfly (Drosophila melanogaster) Pelle and related animal cytoplasmic kinases (Shiu and Bleecker, 2001). In cases in which they have been tested, these kinases have been shown to have specificity for phosphorylation on Ser/Thr residues (Shiu and Bleecker, 2001).The plant RLKs can be grouped into more than 21 structural classes based on their extracellular domains (Shiu and Bleecker, 2001). The LecRKs represent one such class in which the extracellular domain is related to legume lectins. The Arabidopsis genome contains at least 42 LecRK sequences that have been grouped into three classes (A-C) plus an additional nine sequences of related soluble lectins (Barre et al., 2002). Searches in plant databases reveal that LecRK genes are widespread in higher plants, but apart from Arabidopsis (Hervé et al., 1996,...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

et al. 2003

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“…LecRKs are involved in biotic and abiotic stress signal transduction. The poplar LecRK gene LPK and the Arabidopsis LecRK gene LecRK-a1/LecRK-V.5 are induced by wounding and senescence, and Arabidopsis LecRK2/ LecRK-I.3 is induced by salt stress (Nishiguchi et al, 2002;Riou et al, 2002;He et al, 2004). LecRK members of the Arabidopsis LecRK-VI clade are redundant negative regulators of the ABA response during seed germination (Xin et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

The Lectin Receptor Kinase-VI.2 Is Required for Priming and Positively Regulates Arabidopsis Pattern-Triggered Immunity

et al. 2012

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Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as b-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI.2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and BOTRYTIS-INDUCED KINASE1 phosphorylation. In addition, complex formation between the MAMP receptor FLAGELLIN SENSING2 and its signaling partner BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming.

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Lectin Receptor like Kinase

Passricha

Saifi

Negi³

et al. 2020

Protein Kinases and Stress Signaling in Plants

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Expression of an Arabidopsis lectin kinase receptor gene, lecRK-a1, is induced during senescence, wounding and in response to oligogalacturonic acids

Cited by 63 publications

References 35 publications

Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

The Lectin Receptor Kinase-VI.2 Is Required for Priming and Positively Regulates Arabidopsis Pattern-Triggered Immunity

Lectin Receptor like Kinase

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