Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in xenopus embryos

Amaya, Enrique; Musci, Thomas J.; Kirschner, Marc W.

doi:10.1016/0092-8674(91)90616-7

Cited by 1,050 publications

(596 citation statements)

References 45 publications

Supporting

Mentioning

561

Contrasting

Unclassified

Order By: Relevance

“…Fgfs and retinoic acid are other signalling molecules that can affect anterior-posterior patterning. Antagonizing Fgf signalling by expression of dominant negative receptors prevents trunk and tail development in Xenopus and zebrafish (Amaya et al, 1991;Griffin et al, 1995), which suggested that Fgfs may posteriorize the embryo. However, Fgf beads do not mimic the posteriorizing effects of the lateral germ ring grafts of Woo and Fraser (1997).…”

Section: Role Of Wnt Signalling and Its Repression In Brain Patterningmentioning

confidence: 99%

Constructing the hindbrain: Insights from the zebrafish

Moens¹,

Prince²

2002

Developmental Dynamics

197

179

View full text Add to dashboard Cite

The hindbrain is responsible for controlling essential functions such as respiration and heart beat that we literally do not think about most of the time. In addition, cranial nerves projecting from the hindbrain control muscles in the jaw, eye, and face, and receive sensory input from these same areas. In all vertebrates that have been studied, the hindbrain passes through a segmented phase shortly after the neural tube has formed, with a series of seven bulges-the rhombomeres-forming along the anterior-posterior extent of the neural tube. Our current understanding of vertebrate hindbrain development comes from integrating data from several model systems. Work on the chick has helped us to understand the cell biology of the rhombomeres, whereas the power of mouse molecular genetics has allowed investigation of the molecular mechanisms underlying their development. This review focuses on the special insights that the zebrafish system has provided to our understanding of hindbrain development. As we will discuss, work in the zebrafish has elucidated inductive events that specify the presumptive hindbrain domain and has identified genes required for hindbrain segmentation and the specification of segment identities.

show abstract

Section: Role Of Wnt Signalling and Its Repression In Brain Patterningmentioning

confidence: 99%

Constructing the hindbrain: Insights from the zebrafish

Moens¹,

Prince²

2002

Developmental Dynamics

197

179

View full text Add to dashboard Cite

show abstract

“…In vertebrates, FGF signals are involved in embryonic growth, the establishment of mesoderm and neural tissues and the anteroposterior patterning of the trunk and tail region of the body (Slack 1994;Doniach 1995;Yamaguchi & Rossant 1995;Yamada & Modak 1998). Inhibition of FGF signaling by the dominant-negative forms of FGF receptors (FGFR) produces deficiencies in the posterior structure and results in embryos with reduced mesoderm (Amaya et al 1991;Yamaguchi et al 1994;Griffin et al 1995). The dominant negative FGFR also inhibits neural induction in Xenopus animal cap explants by Noggin and the dominant-negative bone morphogenic protein (BMP) receptor, and by organizers (Launay et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Role of the FGF and MEK signaling pathway in the ascidian embryo

2001

View full text Add to dashboard Cite

In the ascidian embryo, a fibroblast growth factor (FGF)-like signal from presumptive endoderm blastomeres between the 32-cell and early 64-cell stages induces the formation of notochord and mesenchyme cells. However, it has not been known whether endogenous FGF signaling is involved in the process. Here it is shown that 64-cell embryos exhibit a marked increase in endogenous extracellular signal-regulated kinase (ERK/MAPK) activity. The increase in ERK activity was reduced by treatment with an FGF receptor 1 inhibitor, SU5402, and a MEK (ERK kinase/MAPKK) inhibitor, U0126. Both drugs blocked the formation of notochord and mesenchyme when embryos were treated at the 32-cell stage, but not at the 2-or 110-cell stages. The dominant-negative form of Ras also suppressed notochord and mesenchyme formation. Both inhibitors suppressed induction by exogenous basic FGF. These results suggest that the FGF signaling cascade is indeed necessary for the formation of notochord and mesenchyme cells during ascidian embryogenesis. It is also shown that FGF signaling is required for formation of the secondary notochord, secondary muscle and neural tissues, and at least ERK activity is necessary for the formation of trunk lateral cells and posterior endoderm. Therefore, FGF and MEK signaling are required for the formation of various tissues in the ascidian embryo.

show abstract

“…The use of this dominantnegative approach has been successfully reported in transfected NIH3T3 cells (Li et al, 1994), and e cient inactivation of FGFR have been obtained in injected xenopus oocytes (Amaya et al, 1991) and also in transgenic mice where a dominant-negative mutant was targeted to the epidermis (Werner et al, 1993). In the present work, by using a dominant-negative form of FGFR1 (Flg receptor) we have obtained a complete inactivation of the FGFR2b splice variant of the endogenous FGFR2 present on the NBT-II cell surface.…”

Section: Direct Fgf-1/fgfr Signalization Is Not Involved In the Commumentioning

confidence: 99%

The community effect in FGF-1 mediated tumor progression of a rat bladder carcinoma does not involve a direct paracrine signaling

1999

View full text Add to dashboard Cite

A community e ect was found to occur between heterogeneous tumor cell populations leading to an overall increased tumorigenicity without a clonal dominance of the more tumorigenic clone. In the rat bladder carcinoma cell line NBT-II, this e ect appears mediated by the Fibroblast Growth Factor-1 (FGF-1) through either a direct or an indirect signaling pathway. Neovascularization induced by FGF-1 was found not to be responsible for the community e ect. The present study shows that the community e ect does not involve a direct FGF-1 signaling since tumor cells expressing a dominant-negative FGF receptor mutant were still responding to the highly tumorigenic FGF-1 expressing cells. Tumors arising from inoculates of the FGF-1 producing NBT-II cells mixed with non tumorigenic epithelial MDCK cells contain only the tumorigenic cells indicating that MDCK cells may exerce a helper e ect for the growth of the tumor not dependant on their own growth. Therefore the helper function of MDCK cells must be distinguished from a community e ect where the contribution of low tumorigenic cells not only provides an in vivo growth advantage to few highly tumorigenic cells but become themselves highly tumorigenic indicating that the community e ect may require cell-cell speci®c cooperativity independent from an helper e ect.

show abstract

Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in xenopus embryos

Cited by 1,050 publications

References 45 publications

Constructing the hindbrain: Insights from the zebrafish

Constructing the hindbrain: Insights from the zebrafish

Role of the FGF and MEK signaling pathway in the ascidian embryo

The community effect in FGF-1 mediated tumor progression of a rat bladder carcinoma does not involve a direct paracrine signaling

Contact Info

Product

Resources

About