Expression of a bifunctional green fluorescent protein (GFP) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape (Vitis vinifera)

Z, Li; Subramanian, Jayasankar; Gray, D. J.

doi:10.1016/s0168-9452(01)00336-3

Cited by 82 publications

(61 citation statements)

References 27 publications

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“…Strong and constitutive promoters are required to engineer pest resistance in crop plants so that transgene can be expressed to confer pest resistance (Li et al 2001). Some promoters can drive phloem specific gene expression in tobacco and other small grain crops (Sugaya et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Spider toxin (Hvt) gene cloned under phloem specific RSs1 and RolC promoters provides resistance against American bollworm (Heliothis armigera)

et al. 2011

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Spider venoms are neurotoxin proteins that can kill insects. Spider toxin Hvt gene was cloned under two phloem specific RSs1 and RolC promoters, transformed into tobacco plants through Agrobacterium-mediated transformation and tested against Heliothis armigera larvae. Transgenic plants were confirmed through PCR. First instar larvae of H. armigera were released on detached leaves of transformed and non-transformed plants. Insect bioassays showed 93-100% mortality of H. armigera larvae within 72 h on the leaves of transgenic plants while all larvae survived and continued feeding on detached leaves from non-transformed control plants. The Hvt gene expressing under phloem specific RSs1 and RolC promoters could therefore be used for developing H. armigera-resistant, genetically-modified crops.

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Section: Introductionmentioning

confidence: 99%

Spider toxin (Hvt) gene cloned under phloem specific RSs1 and RolC promoters provides resistance against American bollworm (Heliothis armigera)

et al. 2011

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“…For this purpose, the green fluorescent protein (GFP) has been widely used for transformation experiments, and various GFP mutants have been developed (Stewart 2001). As GFP can visualize and simplify the tracking of transgenic events, transformation by the GFP gene has recently been applied to recalcitrant or economically important species to establish a new and efficient transformation system (Ghorbel et al 1999;Iocco et al 2001;Li et al 2001;Winfield et al 2001) or to study trans-gene regulation (Chytilova et al 1999;Weld et al 2001;Wymer et al 2001). In the investigation reported here, we used the sGFP (S65T) gene, one of the GFP mutants (Chiu et al 1996;Niwa et al 1999) as a marker gene to trace and evaluate the delivery of a transgene and successfully produced the first genetically modified verbena plants by Agrobacterium-mediated transformation.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of transformed verbena (Verbena × hybrida) by Agrobacterium tumefaciens

et al. 2003

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Verbena (Verbena x hybrida), an important floricultural species, was successfully regenerated from stem segments on Murashige and Skoog's basal medium supplemented with thidiazuron and indole-3-acetic acid. A transformation system was developed using cvs. Temari Scarlet, Temari Sakura, Tapien Rose and TP-P2. Agrobacterium tumefaciens strain Agl0 harboring the sGFP gene was infected into stem segments. Transformation efficiency was improved by evaluating and manipulating the age of the plant material, the concentration of kanamycin in the medium during selection, and the length of the culture period in the dark. After 2-3 months of culture on the selection medium, GFP-positive shoots were obtained in all four of the cultivars tested. These shoots were successfully acclimated and set flowers within 2-3 months in a greenhouse. GFP was expressed in all of the organs including the floral parts. Stable genomic transformation was confirmed by Southern blot analysis. No morphological differences were observed between the transformed plants and their host plants.

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“…In this study, 2XCaMV35S promoter was used as a driving promoter of hpt and mgfp genes with the aim to exhibit strong constitutive activity in oil palm tissues and subsequently result in efficient selection for stable transgenic oil palm. Higher expression also has been demonstrated in transgenic plants regulated by 2XCaMV35S promoter such as grape (Li et al, 2001) and citrus (Benyon et al, 2013). Constitutively transgene expression driven by this promoter also resulted in the production of stable transgenic plants for soyabean (Paz et al, 2004), strawberries (Qin et al, 2008), rice (Zheng et al, 2009), maize (Reyes et al, 2010) and citrus (Mondal et al, 2012).…”

Section: Construction Of Transformation Vectorsmentioning

confidence: 98%

“…The transgene expression regulated by 2XCaMV35S promoter has been demonstrated to be highly active in most tissues including roots, mature leaves and shoots. The promoter has been used for the production of stable transgenic plants such as grape (Li et al, 2001), citrus (Benyon et al, 2013), soyabean (Paz et al, 2004), rice (Zheng et al, 2009) and maize (Reyes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Construction of a Vector Containing Hygromycin (Hpt) Gene Driven by Double 35s (2xcamv35s) Promoter for Oil Palm Transformation

Bahariah¹

2017

JOPR

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Transformation vector construction is one of the important disciplines for plant genetic transformation studies. A series of vector consisting of hygromycin phosphotransferase (hpt) gene as the selective marker and green fluorescent protein (GFP) as the visual reporter gene, under the control of double cauliflower mosaic virus 35S (2XCaMV35S) promoter has been engineered for transformation into oil palm cells. These genes were cloned into different types of cloning and expression vectors. The cloning was carried out by using restriction enzyme digestion and ligation method. Five intermediate vectors have been created for insertion of 2XCaMV35S-HPT-35ST and 2XCaMV35S-GFP-35ST into modified pBINPLUS backbone vector for particle bombardment and

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Expression of a bifunctional green fluorescent protein (GFP) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape (Vitis vinifera)

Cited by 82 publications

References 27 publications

Spider toxin (Hvt) gene cloned under phloem specific RSs1 and RolC promoters provides resistance against American bollworm (Heliothis armigera)

Spider toxin (Hvt) gene cloned under phloem specific RSs1 and RolC promoters provides resistance against American bollworm (Heliothis armigera)

Regeneration of transformed verbena (Verbena × hybrida) by Agrobacterium tumefaciens

Construction of a Vector Containing Hygromycin (Hpt) Gene Driven by Double 35s (2xcamv35s) Promoter for Oil Palm Transformation

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