Expression of 11 members of the BCL‐2 family of apoptosis regulatory molecules during human preimplantation embryo development and fragmentation

Metcalfe, Anthony D.; Hunter, Helen; Bloor, Debra; Lieberman, Brian A.; Picton, Helen M.; Leese, Henry J.; Kimber, Susan J.; Brison, Daniel R.

doi:10.1002/mrd.20055

Cited by 92 publications

(95 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The peak expression of anti-apoptotic factor BCLXL coincided with start of ZGA in in vivo developed rabbit embryo similar to the reports in mouse, human and cattle embryos [27,29,46,47]. Housekeeping genes and apoptotic regulators were reported to peak at the ZGA in bovine and murine embryos [48,49].…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Gibence

Brahmasani

Mahesh

et al. 2014

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The levels and timing of expression of genes like BCLXL, HDAC1 and pluripotency marker genes namely, OCT4, SOX2, NANOG and KLF4 are known to influence preimplantation embryo development. Despite this information, precise understanding of their influence during preimplantation embryo development is lacking. The present study attempts to compare the expression of these genes in the in vivo and in vitro developed preimplantation embryos. Methods The in vivo and in vitro developed rabbit embryos collected at distinct developmental stages namely, pronuclear, 2 cell, 4 cell, 8 cell, 16 cell, Morula and blastocyst were compared at the transcriptional and translational levels using Real Time PCR and immunocytochemical studies respectively. Results The study establishes the altered levels of candidate genes at the transcriptional level and translational level with reference to the zygotic genome activation (ZGA) phase of embryo development in the in vivo and in vitro developed embryos. The expression of OCT4, KLF4, NANOG and SOX2 genes were higher in the in vitro developed embryos whereas and HDAC1 was lower. BCLXL expression had its peak at ZGA in in vivo developed embryos. Protein expression of all the candidate genes was observed in the embryos. BCLXL, KLF4 and NANOG exhibited diffused localisation whereas HDAC1, OCT4, and SOX2 exhibited nuclear localisation. Conclusions This study leads to conclude that BCLXL peak expression at the ZGA phase may be a requirement for embryo development. Further expression of all the candidate genes was influenced by ZGA phase of development at the transcript level, but not at the protein level.

show abstract

Section: Discussionsupporting

confidence: 82%

“…Among which the BAX/BCLXL ratio indicates survival or death of a cell [26,27]. BCLXL was required for cell survival and development through the ZGA phase in murine preimplantation embryos and the microinjection of BCLXL could rescue embryos and aid in ZGA [28,29].…”

Section: Introductionmentioning

confidence: 99%

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Gibence

Brahmasani

Mahesh

et al. 2014

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…However, the capability of response gradually decreased along with the embryonic development. The Bcl-2 gene family includes pro-apoptotic (Bax, Bad, Bak, Bokl, Bcl-xs, Bik, and Bid) and anti-apoptotic (Bcl-2, Bcl-xl, Mcl-1, and Bcl-w) subgroups and plays an important role in regulation of cell apoptosis during embryo development [37]. In our study, IVF embryos yielded significantly higher expression of Bcl-2 mRNA compared to SCNT embryos after 16-cell stage.…”

Section: Discussionmentioning

confidence: 99%

Analysis of apoptosis and methyltransferase mRNA expression in porcine cloned embryos cultured in vitro

Ren

Lü

et al. 2010

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The purpose of this study was to investigate the relationship of porcine somatic cell nuclear transfer (SCNT) embryo developmental competence with embryonic cell apoptosis and DNA methylation. Methods The apoptotic incidence was examined via comet assay, and the mRNA expression of genes implicated in apoptosis (Bcl-2) and DNA methylation (Dnmt1, Dnmt3a) was determined using real-time RT-PCR. Results Comet assay showed that the SCNT embryos exhibited significantly higher apoptotic rate at 2-cell stage (8.3% versus 2.1%, P<0.05), 16-cell stage (27.3% versus 19.2%, P<0.05) and morula (37.5% versus 26.9, P<0.05) compared with IVF embryos. Compared with IVF embryos, a higher Bcl-2 mRNA expression pattern was observed in SCNT embryos before the 8-cell stage and differed significantly at 2-and 4-cell stages (P<0.05). After the 16-stage, Bcl-2 mRNA expression pattern became significantly lower in SCNT group (P <0.05). The relative expression level of Dnmt1 mRNA showed a higher expression level in oocytes, then sharply decreased and started to increase slightly after the 8-cell (IVF embryos) or 16-cell stage (SCNT embryos). Dnmt1 mRNA expression in IVF embryos appeared to have been lower than that of SCNT group before 16-cell stage embryos, especially at 4-and 8-cell stages (P<0.05). Although a trend for a similar increase of Dnmt3a expression was observed in IVF and SCNT embryos after 8-cell embryos, SCNT group resulted in much higher Dnmt3a mRNA abundance compared with the IVF group, particularly after 16-cell embryos (P<0.05).Conclusions The results showed that low efficiency of porcine SCNT technology may be associated with either embryonic apoptosis or incomplete reprogramming of donor nuclear caused by abnormal Dnmts mRNA expression.

show abstract

“…Interestingly, recent research has discovered a third pathway of apoptosis in which endonuclease G, released from mitochondria and translocated to the nucleus, can fragmentize DNA independently of active caspases (Li et al 2001). Nevertheless, the importance of caspases in the apoptotic mechanism cannot be neglected, since in several species, such as human, mouse, and rat, detection of caspase mRNAs and/or active caspase proteins has been associated with apoptosis during embryo development, mostly in a stage-specific manner (Exley et al 1999, Hinck et al 2001, Spanos et al 2002, Jurisicova et al 2003, Metcalfe et al 2004. From the results of both our experiments, it was obvious that the percentage of caspase-positive cells declined in advanced developmental stages.…”

Section: Discussionmentioning

confidence: 99%

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Vandaele

Mateusen²,

Maes³

et al. 2007

Reproduction

View full text Add to dashboard Cite

Embryo quality is most frequently evaluated at the blastocyst stage, although quality parameters further back along the developmental axis, such as early developmental kinetics or oocyte quality, can be equally valuable. Despite the fact that previous studies in bovine have linked oocyte diameter and early developmental kinetics with blastocyst formation and viability, their relation with the incidence of apoptosis during embryo development remains relatively unexplored. Therefore, we related noninvasive parameters of oocyte and embryo quality, such as embryo kinetics, embryo morphology, and oocyte diameter, to the incidence of apoptosis throughout embryo development using fluorescent detection of active caspase-3 and -7. First, bovine in vitro embryos were selected according to developmental kinetics and morphology at four set times during culture and subjected to fluorescent detection of active caspase-3 and -7. Caspase activity was significantly higher in slow developing embryos in comparison with fast cleavers (P!0.05), but was not related to embryo morphology. Second, bovine oocytes were divided into three groups on the basis of oocyte diameter and the resulting embryos were used for staining at the same four set times. Caspase activity was significantly higher in embryos derived from growing oocytes compared with those of fully grown oocytes at 45, 80, and 117 hours post-insemination (hpi; P!0.05), but not at 168 hpi. Reproduction (2007) 133 709-718

show abstract

Expression of 11 members of the BCL‐2 family of apoptosis regulatory molecules during human preimplantation embryo development and fragmentation

Cited by 92 publications

References 70 publications

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Analysis of apoptosis and methyltransferase mRNA expression in porcine cloned embryos cultured in vitro

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Contact Info

Product

Resources

About