Expression and purification of rat recombinant aminopeptidase B secreted from baculovirus-infected insect cells

Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation

“…Although His tag introduction has sometimes caused alterations in protein structure (28), inhibition of enzyme activity, or toxicity (29,30), the majority of tagged proteins retain their native structural integrity and biological activity (31). Because it had been expressed in pET28a, the EstS1 enzyme had an N-terminal His tag.…”

Heterologous expression and N-terminal His-tagging processes affect the catalytic properties of staphylococcal lipases: A monolayer study

“…However, the use of affinity tags for protein purification has some obvious drawbacks, such as changes in the protein conformation [38], undesirable flexibility [39], misfolding and/or loss of activity [40] or/and the solubility of the protein, which greatly restrict the use of the tag labeling method for X-ray crystallography and other physico-chemical characterization purposes [41][42][43]. As far as lipases are concerned, Quyen et al [44] reported that the wild type lipase from Ralstania sp.…”

The effect of the hexahistidine-tag in the oligomerization of HSC70 constructs

“…Similarly, other amino acids like cysteine and naturally occurring histidine rich regions in host proteins may result in unwanted protein binding during IMAC purification [31]. Additionally, introducing an affinity tag has also been reported to negatively affect the target protein resulting in a change in protein conformation [32], undesired flexibility in structure studies [33], inhibition of enzyme activity, toxicity [34,35] and alteration in biological activity [36].…”