Expression and prognostic significance of MAP4K4 in lung adenocarcinoma

Qiu, Meihua; Qian, Yiming; Zhao, Xiao Guang; Wang, Shou‐Mei; Feng, Xiaohai; Chen, Xin-Fang; Zhang, Shu‐Hui

doi:10.1016/j.prp.2012.06.001

Cited by 44 publications

(37 citation statements)

References 23 publications

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“…F, Western blot analysis showed a significant overexpression of MAP4K4 after transfection with miR-141 inhibitor. Ã , P < 0.05; ÃÃ , P < 0.01. hepatocellular carcinoma (32), lung adenocarcinoma (35), and even pancreatic ductal adenocarcinoma (33). The reported expression level of MAP4K4 was closely related to the overall survival of these patients with cancer, especially those diagnosed with pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

miRNA-141, Downregulated in Pancreatic Cancer, Inhibits Cell Proliferation and Invasion by Directly Targeting MAP4K4

Zhao

Wang

Liu

et al. 2013

Molecular Cancer Therapeutics

138

110

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miRNAs are associated with various types of cancer due to their ability to affect expression of genes that modulate tumorigenesis. In this study, we explored the role of miR-141 in pancreatic cancer. The analysis of clinical characteristics showed that miR-141 was significantly downregulated in tissues and cell lines of pancreatic cancer. Moreover, the decreased miR-141 level was significantly associated with tumor size and TNM stage, as well as lymph node and distant metastasis. Meanwhile, both Kaplan-Meier and multivariate survival analysis showed decreased miR-141 were associated with overall survival. Overexpression of miR-141 in pancreatic cancer cells inhibited cell proliferation, clonogenicity, and invasion; induced G 1 arrest and apoptosis; and enhanced chemosensitivity. To understand how miR-141 mediates the phenotype of pancreatic cancer cells, a bioinformatics tool was used to identify MAP4K4 as a potential target of miR-141. The DualLuciferase reporter gene assay showed that miR-141 binds directly to the 3 0 -untranslated region (3 0 UTR) of MAP4K4 to inhibit MAP4K4 expression. Western blot and quantitative real-time PCR (qRT-PCR) analyses revealed that MAP4K4 expression was inversely correlated with miR-141 expression both in pancreatic cancer samples and cell lines. Knockdown of MAP4K4 inhibited cell proliferation, clonogenicity, and invasion, induced G 1 arrest and apoptosis, and enhanced chemosensitivity. In a nude mouse xenograft model, both overexpression of miR-141 and knockdown of MAP4K4 significantly repressed pancreatic cancer cell growth. Therefore, we conclude that miR-141 targets MAP4K4, acts as a tumor suppressor in pancreatic cancer cells, and may serve as a novel therapeutic agent for miRNA-based pancreatic cancer therapy. Mol Cancer Ther; 12(11); 2569-80. Ó2013 AACR.

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Section: Discussionmentioning

confidence: 99%

miRNA-141, Downregulated in Pancreatic Cancer, Inhibits Cell Proliferation and Invasion by Directly Targeting MAP4K4

Zhao

Wang

Liu

et al. 2013

Molecular Cancer Therapeutics

138

110

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“…MAP4K4, involved in MAPK signaling pathway, has been reported to be frequently overexpressed in several types of human tumors. 41,42 MAP4K4 participates in the initiation and development of cancers by promoting cell proliferation, inhibiting cell apoptosis, promoting angiogenesis, invasion, and metastasis. 21,23 In this study, through bioinformatics analysis, we found that MAP4K4 might be one of the potential targets for miR-194.…”

Section: -37mentioning

confidence: 99%

“…MAPK kinase kinase kinase isoform 4 (MAP4K4) is a relatively novel gene belonging to the mammalian STE20/MAP4K family. It has been reported as an oncogene in multiple forms of cancer 19 with poor prognosis, such as prostate cancer, 20 lung cancer, 21,22 hepatocellular cancer, 21 and pancreatic cancer. 23 However, little is known about the functions of MAP4K4 and not yet been fully clarified in CRC.…”

Section: Introductionmentioning

confidence: 99%

MiR-194, commonly repressed in colorectal cancer, suppresses tumor growth by regulating the MAP4K4/c-Jun/MDM2 signaling pathway

et al. 2015

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“…Presumably, reduced expression would have the opposite effect. MAP4K4 is a serine/threonine kinase that is overexpressed in many cancers where it is implicated in migration and invasion [43] and RBFOX1 is related to the candidate driver RBFOX2 [44]. Zhou et al [45] reported a mutation in RBFOX1 in a colorectal adenoma.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the Genomic Architecture and Mutational Spectrum of a Small Cell Prostate Carcinoma

Scott

Mohr

Ling

et al. 2014

Genes

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We present the use of a series of laboratory, analytical and interpretation methods to investigate personalized cancer care for a case of small cell prostate carcinoma (SCPC), a rare and aggressive tumor with poor prognosis, for which the underlying genomic architecture and mutational spectrum has not been well characterized. We performed both SNP genotyping and exome sequencing of a Virchow node metastasis from a patient with SCPC. A variety of methods were used to analyze and interpret the tumor genome for copy number variation, loss of heterozygosity (LOH), somatic mosaicism and mutations in genes from known cancer pathways. The combination of genotyping and exome sequencing approaches provided more information than either technique alone. The results showed widespread evidence of copy number changes involving most chromosomes including the possible loss of both alleles of CDKN1B (p27/Kip1). LOH was observed for the regions encompassing the tumor suppressors TP53, RB1, and CHD1. Predicted damaging somatic mutations were observed in the retained TP53 and RB1 alleles. Mutations in other genes that may be functionally relevant were noted, especially the recently reported high confidence cancer drivers FOXA1 and CCAR1. The disruption of multiple cancer drivers underscores why SCPC may be such a difficult cancer to manage.

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Expression and prognostic significance of MAP4K4 in lung adenocarcinoma

Cited by 44 publications

References 23 publications

miRNA-141, Downregulated in Pancreatic Cancer, Inhibits Cell Proliferation and Invasion by Directly Targeting MAP4K4

miRNA-141, Downregulated in Pancreatic Cancer, Inhibits Cell Proliferation and Invasion by Directly Targeting MAP4K4

MiR-194, commonly repressed in colorectal cancer, suppresses tumor growth by regulating the MAP4K4/c-Jun/MDM2 signaling pathway

Characterization of the Genomic Architecture and Mutational Spectrum of a Small Cell Prostate Carcinoma

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