Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure

Kiczak, Liliana; Tomaszek, Alicja; Bania, Jacek; Pasławska, Urszula; Zacharski, Maciej; Noszczyk-Nowak, Agnieszka; Janiszewski, Adrian; Skrzypczak, Piotr; Ardehali, Hossein; Jankowska, Ewa A.; Ponikowski, Piotr

doi:10.1155/2013/283856

Cited by 23 publications

(18 citation statements)

References 39 publications

(59 reference statements)

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“…2007;Kiczak et al 2013;Gaggin and Januzzi 2014). Serum BNP, MMP2 and MMP9 were reported as biomarkers of cardiac disease (Šímová et al 2013;Pufulete et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Elevated serum brain natriuretic peptide and matrix metalloproteinases 2 and 9 in Wilson’s disease

et al. 2015

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Wilson's disease (WD) is a disease of copper metabolism characterized by excessive copper deposition in the body. It is reported abnormal copper metabolism has been associated with cardiovascular disease. BNP and MMP2/9 were biomarkers of congestive heart failure (CHF). There is rare study to explore whether serum concentrations of BNP, MMP2, and or MMP9 are altered in patients with WD. In this study we determine whether serum concentrations of brain natriuretic peptide (BNP) and matrix metalloproteinases (MMP) 2 and 9 are increased in patients with WD. Serum BNP, MMP2 and MMP9 were measured by an ELISA in 34 patients with hepatic WD, in 68 patients with neurological WD, and in 33 healthy controls. We found serum BNP levels were higher in patients with neurological WD than in healthy controls (p = 0.033). Serum MMP2 levels were higher in patients with hepatic (p = 0.009) and neurologic (p = 0.0004) WD than in controls. Serum MMP9 levels were higher in patients with neurologic WD than in patients with hepatic WD (p = 0.002) and controls (p = 0.00005), and were higher in patients with hepatic WD than in controls (p = 0.03). Serum BNP levels were negatively correlated with ceruloplasmin (p = 0.017, r = -0.215), while serum (p = 0.019, r = -0.221) and MMP9 (p = 0.011, r = -0.231) in patients with WD were negatively correlated with ceruloplasmin. BNP, MMP2, and MMP9 may reflect the deposition of copper in the heart.

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“…2007;Kiczak et al 2013;Gaggin and Januzzi 2014). Serum BNP, MMP2 and MMP9 were reported as biomarkers of cardiac disease (Šímová et al 2013;Pufulete et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Elevated serum brain natriuretic peptide and matrix metalloproteinases 2 and 9 in Wilson’s disease

et al. 2015

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“…A recent study showed that matrix metalloproteinases 2 and 9 (MMPs), key enzymes responsible for myocardial remodelling during heart disease, are up-regulated in the failing myocardium but not in skeletal muscles in pigs with tachycardia-induced cardiomyopathy (8). MMPs are enzymes found throughout the body that are responsible for remodelling of tissues.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiling of peripheral blood nucleated cells in dogs with and without clinical signs of chronic mitral valve disease

Garncarz

Hulanicka

Parzeniecka-Jaworska

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to demonstrate differences in the gene expression of signalling pathways between healthy dogs and dogs with chronic mitral valve disease in different heart failure groups. Blood samples were collected from 49 dogs of various breeds between 1.4 and 15.2 years of age. Isolated RNA samples were analysed for quality and integrity and the gene expression profile was determined. The study demonstrated that nucleated cells from peripheral blood can be used to assess the status of heart failure in dogs. Furthermore, significant differences in the expression of the genes were noticed between healthy dogs and dogs with clinical signs of chronic mitral valve disease. This is a preliminary non-invasive study showing the feasibility of genetic testing from peripheral blood nucleated cells, which at the same time has made it possible to set the future directions of genetic studies in clinical cases of canine chronic mitral valve disease.

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“…The primer sequences used were GAPDH forward: 5′-CCTTCATTGACCTCCACTACA TGGT-3′ and reverse: 5′-CCACAACATACGTAGCACCACGATC-3′, and Ngal forward: 5′-TTAAGAAATACTCTGGATTGC-3′ and reverse: 5′-TACTCTTGGTTGTTGGAAAC-3′. The primers are located in separate exons to prevent the amplification of genomic DNA (Kiczak et al 2013). Relative quantification of targeted gene expression was calculated using the comparative threshold cycle method (Livak and Schmittgen 2001).…”

Section: Expression Levels Of Ngal Mrnamentioning

confidence: 99%

The Piezo Actuator-Driven Pulsed Water Jet System for Minimizing Renal Damage after Off-Clamp Laparoscopic Partial Nephrectomy

Kamiyama

Yamashita

Nakagawa

et al. 2017

Tohoku J. Exp. Med.

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In the setting of partial nephrectomy (PN) for renal cell carcinoma, postoperative renal dysfunction might be caused by surgical procedure. The aim of this study was to clarify the technical safety and renal damage after off-clamp laparoscopic PN (LPN) with a piezo actuator-driven pulsed water jet (ADPJ) system. Eight swine underwent off-clamp LPN with this surgical device, while off-clamp open PN was also performed with radio knife or soft coagulation. The length of the removed kidney was 40 mm, and the renal parenchyma was dissected until the renal calyx became clearly visible. The degree of renal degeneration from the resection surface was compared by Hematoxylin-Eosin staining and immunostaining for 1-methyladenosine, a sensitive marker for the ischemic tissue damage. The mRNA levels of neutrophil gelatinase-associated lipocalin (Ngal), a biomarker for acute kidney injury, were measured by quantitative real-time PCR. Off-clamp LPN with ADPJ system was successfully performed while preserving fine blood vessels and the renal calix with little bleeding. In contrast to other devices, the resection surface obtained with the ADPJ system showed only marginal degree of ischemic changes. Indeed, the expression level of Ngal mRNA was lower in the resection surface obtained with the ADPJ system than that with soft coagulation (p = 0.02). Furthermore, using the excised specimens of renal cell carcinoma, we measured the breaking strength at each site of the human kidney, suggesting the applicability of this ADPJ to clinical trials. In conclusion, off-clamp LPN with the ADPJ system could be safely performed with attenuated renal damage.

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Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure

Cited by 23 publications

References 39 publications

Elevated serum brain natriuretic peptide and matrix metalloproteinases 2 and 9 in Wilson’s disease

Elevated serum brain natriuretic peptide and matrix metalloproteinases 2 and 9 in Wilson’s disease

Transcriptomic profiling of peripheral blood nucleated cells in dogs with and without clinical signs of chronic mitral valve disease

The Piezo Actuator-Driven Pulsed Water Jet System for Minimizing Renal Damage after Off-Clamp Laparoscopic Partial Nephrectomy

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