Expression and clinical role of TCTP in epithelial ovarian cancer

Abstract

The aim of this study is to investigate the potential role and prognostic significance of translationally controlled tumor protein (TCTP) in human epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of TCTP in eight fresh EOC tissues. Immunohistochemistry was performed on formalin-fixed paraffin-embedded sections of 119 cases of ovarian cancers. Kaplan-Meier method indicated the relation between TCTP and EOC patients' overall survival rate. Starvation and re-feeding was used to assess cell cycle. Knocking down of TCTP and CCK8 assay showed the role of TCTP in HO8910 cell cycle. We found that TCTP was overexpressed in carcinoma tissues compared with normal tissues. Immunohistochemistry revealed that TCTP expression was significantly associated with clinicopathologic variables. Kaplan-Meier analysis revealed that high TCTP expression was significantly related to poor prognosis of the patients. Starvation and re-feeding suggested TCTP played a critical role in HO8910 cell proliferation. Interference of TCTP and CCK8 assay showed that the TCTP-siRNA treated HO8910 cells grew more slowly than the control group. CCK-8 assays and terminal-deoxynucleoitidyl transferase mediated nick end labeling assays were also performed to demonstrate the cisplatin could inhibit the survival of HO8910 cells and promote their apoptosis. All the experiments we have done showed that TCTP could promote the progression of EOC and reduce the sensitiveness of HO8910 cells to cisplatin.

Fortilin: A Potential Target for the Prevention and Treatment of Human Diseases

“…Fortilin TCTP overexpression leads to systemic hypertension through its direct binding to and inhibition of Na,K-ATPase [95, 96], while it is not clear how fortilin TCTP contributes to pulmonary arterial hypertension [97]. Fortilin TCTP plays an important role in a number of malignant neoplasms including breast [27, 99, 100], gastric [102], colorectal [107, 108], liver [109], lung [110], oral [111], ovarian [112], and prostate [113, 114] cancers and central and peripheral nervous system tumors including glioma [19, 30, 116], neurofibromatosis [119], and neuroblastomas [120]. It is likely that tumorigenicity of fortilin TCTP is mediated by its ability to protect malignant cells against apoptosis [1, 12–14, 16, 17, 20, 22, 55, 56].…”

“…Chen and coworkers immunostained 119 formalin-fixed, paraffin-embedded epithelial ovarian cancer (EOC) tissues and found that fortilin TCTP expression was significantly and positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) histological grade and shorter survival [112]. …”

Phospho-TCTP and Dihydroartemisinin: A Novel Therapeutic Opportunity in Advance Breast Cancer

“…Despite numerous reports suggesting relevant functions of TCTP in the context of tumor biology, and many findings highlighting that TCTP overexpression is associated with tumor progression and poor clinical outcome in many poorly differentiated tumors [4][5][6][7], the precise role of this protein is still a matter of debate. An important observation, which will be helpful to elucidate its implication in cell survival mechanisms, is that TCTP is a direct substrate of the serine/threonine kinase polo-like kinase 1 (PLK1), a crucial player in cell-cycle regulation during mitosis [8].…”

The role of Cyclin G1 in cellular proliferation and apoptosis of human epithelial ovarian cancer

“…After transfected with sh Cyclin G1 or control-sh RNA 24 h, cells were seeded into 96-well plates at a concentration of 2 9 10 4 cells per well in volumes of 90 ll and grew overnight (Chen et al 2015). 10 ll CCK8 reagents (Dojindo, Kumamoto, Japan) were then added into every well at different time points and then incubated for 2 h in dark environment.…”