Expression analysis of circulating plasma long noncoding RNAs in colorectal cancer: The relevance of lncRNAs ATB and CCAT1 as potential clinical hallmarks

Abstract

Long noncoding RNAs (lncRNAs) have been demonstrated to regulate a variety of cell processes and involve in the development and progression of colorectal cancer (CRC).Recently, the circulating lncRNAs have emerged as minimally invasive biomarkers for cancer diagnosis and prognosis. We aimed to examine the plasma expression level of long noncoding RNAs lnc-ATB, lnc-CCAT1, and lnc-OCC-1 in CRC patients and evaluate the clinical values. A total of 74 pretreatment CRC and 74 healthy blood biopsies were subjected to differentially evaluate the expression levels of three lncRNAs (OCC-1, CCAT1, and ATB). Briefly, after plasma separation and total RNA extraction, RNAs were reversely transcribed to complementary DNA followed by amplification using a quantitative real-time polymerase chain reaction technique for lncRNA expression analysis. The results showed that the expression levels of lnc-ATB (p < 0.001) and CCAT1 (p = 0.024), but not OCC-1 (p = 0.24), were significantly upregulated in the CRC compared with the healthy group. The calculated AUC of ROC was 0.78 (95% confidence interval [CI]: 0.811-0.94) for lnc-ATB and 0.64 (95% CI: 0.811-0.94) for CCAT1, which were indicative of a high discriminatory power (p < 0.001). The highest accuracy for lncRNA-ATB was obtained at a cutoff point of 2.5, which corresponded to sensitivity and specificity of 82% and 75%, respectively.Our results suggested a significant accuracy of lncRNA-ATB and lncRNA-CCAT1 in distinguishing CRC patients from healthy individuals. K E Y W O R D SATB, CCAT1, colorectal cancer, long noncoding RNA, OCC-1, plasma

Effect of alkylglycerone phosphate synthase on the expression levels of lncRNAs in glioma cells and its functional prediction

“…lncRNAs were once considered to be a noisy byproduct of the transcription process (24). However, previous studies have reported that lncRNAs serve an important role at the RNA level in regulating cell proliferation, differentiation, the maintenance of pluripotent stem cells and cancer pathogenesis (25,26). The present study aimed to identify the lncRNAs associated with glioma, and subsequently used bioinformatics to analyze the biological functions and signal transduction pathways involved in the regulation of the lncRNAs identified.…”

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

“…The higher expression of UNC5B-AS1, MATAL1 and n340790 was shown to distinguish the tumor and normal samples with AUC values of 0.932, 0.632 and 0.845, respectively (127,130,131). With the increasing identification of diagnostic circulating lncRNAs in different cancers (173)(174)(175)(176)(177), we expect to see the utilization of circulating lncRNAs to optimize diagnostic accuracy of thyroid nodules in the future.…”

Clinical diagnostic value of long non-coding RNAs in Colorectal Cancer: A systematic review and meta-analysis

“…It is reported that several lncRNAs are aberrantly expressed in tissue or serum from colorectal cancer patients. Some studies have reported that MEG3 [9], lnc-ATB [10] and BLACAT1 [11] were up-expressed in colorectal cancer plasma, while NKILA [7] and HOTAIRM1 [12] were down-regulated in colorectal cancer tissue. A sensitivity of 37.16% and a specificity of 88.75% for CEA were significantly lower than that for exosomal CRNDE-h (P < 0.001) [13].…”

“…The expression levels of LncRNAs were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Seven types of lncRNAs were recognized as tumor promoters [10,11,13,14,[21][22][23][24][25] and six were tumor suppressors [7,9,[26][27][28][29] in CRC patients. The associations between lncRNAs and clinicopathologic parameters in CRC patients are shown in Table 1.…”