Exposures of<i>Homarus americanus</i>Shell to Three Bacteria Isolated from Naturally Occurring Epizootic Shell Disease Lesions

Quinn, Robert A.; Metzler, Anita; Smolowitz, Roxanna; Tlusty, Michael F.; Chistoserdov, Andrei Y.

doi:10.2983/035.031.0208

Cited by 58 publications

(69 citation statements)

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“…Although this OTU was not a member of the affected shell core microbiome, it was present in 13 of the 16 affected shell samples and was present at relatively high abundance in 7 of those samples. This OTU, genus Loktanella, which shared 100% sequence identity with bacteria recovered from other diseased lobster shells (Quinn et al 2012a), may therefore be in fecting the shell alongside early colonizers, and, in some instances, growing in abundance as LMSD progresses.…”

Section: Discussionmentioning

confidence: 97%

“…Several studies have reported Aquimarina 'homaria' as a potential causative agent in shell disease, including a bacterial challenge study that was able to recover this bacterium from lobsters with lesions (Quinn et al 2012a) and a next-generation sequencing study that examined healthy and diseased shell (Meres et al 2012). Although we only recovered a single OTU that was associated with the genus Aquimarina (OTU 18), we recovered several other OTUs, including Lutimonas, that were from the Flavobacteria family (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…OTU 18, the second most abundant OTU on the affected shell (see Table S1), shared 100% sequence identity with a representative from the genus Aquimarina (Esteves et al 2013), and 99% sequence identity with bacteria re covered from lobster shells after having been exposed to Aquimarina 'homaria' (Quinn et al 2012a). Aquimarina 'homaria' has been identified by several studies as a potential causative agent of epizootic shell disease as it can readily be recovered from lesions , 2012, Quinn et al 2012a. The abundance of OTU 18 on the affected shell in this study (12%, Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used a laboratory-based model that has been used in numerous studies of lobster shell disease (Quinn et al 2012a,b, Tlusty & Metzler 2012 to characterize the bacterial communities recovered from lobster shells. Our objectives were to (1) compare healthy and diseased areas of the lobster shell, (2) investigate how these communities changed spatially and temporally as the disease progressed, and (3) identify any associated dysbiotic shifts that occurred in the bacterial community.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

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Fine-scale transition to lower bacterial diversity and altered community composition precedes shell disease in laboratory-reared juvenile American lobster

Feinman¹,

Martínez²,

Bowen³

et al. 2017

Dis. Aquat. Org.

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The American lobster Homarus americanus supports a valuable commercial fishery in the Northeastern USA and Maritime Canada; however, stocks in the southern portion of the lobster's range have shown declines, in part due to the emergence of shell disease. Epizootic shell disease is a bacterially induced cuticular erosion that renders even mildly affected lobsters unmarketable because of their appearance, and in more severe cases can cause mortality. Despite the importance of this disease, the associated bacterial communities have not yet been fully characterized. We sampled 2 yr old, laboratory-reared lobsters that displayed signs of shell disease at the site of disease as well as at 0.5, 1, and 1.5 cm away from the site of disease to determine how the bacterial community changed over this fine spatial scale. Illumina sequencing of the 16S rRNA gene revealed a distinct bacterial community at the site of disease, with significant reductions in bacterial diversity and richness compared to more distant sampling locations. The bacterial community composition 0.5 cm from the site of disease was also altered, and there was an observable decrease in bacterial diversity and richness, even though there were no signs of disease at that location. Given the distinctiveness of the bacterial community at the site of disease and 0.5 cm from the site of disease, we refer to these communities as affected and transitionary, and suggest that these bacteria, including the previously proposed causative agent, Aquimarina 'homaria', are important for the initiation and progression of this laboratory model of shell disease.KEY WORDS: American lobster · Shell disease · Bacterial communities · Microbiome · High throughput sequencing · 16S rRNA gene Resale or republication not permitted without written consent of the publisherDis Aquat Org 124: [41][42][43][44][45][46][47][48][49][50][51][52][53][54] 2017 2005). Due to the physical severity of epizootic shell disease, even mildly affected lobsters are generally considered unmarketable due to the unappealing appearance of their shells, thus affecting the value of this important commercial fishery .In addition to aesthetic changes associated with epizootic shell disease, animals can also suffer from decreased health ) and increased mortality . Lobsters affected by shell disease are energetically compromised, with disruptions to their metabolism and hormone signaling (Tarrant et al. 2012). They also tend to have high levels of the molting hormone ecdysone, which can disrupt their reproductive cycle (Laufer et al. 2005). These physiological changes may in turn influence population dynamics, particularly since disease prevalence among egg-bearing females can be as high as 70% (Castro & Somers 2012). The recent decline in the southern New England American lobster population has been positively associated with an in crease in epizootic shell disease , Howell 2012, highlighting the importance of understanding the ecological dynamics of this disease.Despite the importance of epizootic ...

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

See 2 more Smart Citations

Fine-scale transition to lower bacterial diversity and altered community composition precedes shell disease in laboratory-reared juvenile American lobster

Feinman¹,

Martínez²,

Bowen³

et al. 2017

Dis. Aquat. Org.

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“…The condition was termed 'epizootic shell disease' (ESD) (Smolowitz et al 2005). A potential causative agent has recently been identified, the bacteria Aquimarina homaria (Quinn et al 2012), although other co-infecting pathogens and conditions (environmental or physiological) confound a simple cause−effect relationship and potentially increase lobster susceptibility to ESD. At present, therefore, ESD is considered as syndromic.…”

mentioning

confidence: 99%

Disease effects on lobster fisheries, ecology, and culture: overview of DAO Special 6

Behringer¹,

Butler²,

Stentiford³

2012

Dis. Aquat. Org.

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Lobsters are prized by commercial and recreational fishermen worldwide, and their populations are therefore buffeted by fishery practices. But lobsters also remain integral members of their benthic communities where predator−prey relationships, competitive interactions, and host−pathogen dynamics push and pull at their population dynamics. Although lobsters have few reported pathogens and parasites relative to other decapod crustaceans, the rise of diseases with consequences for lobster fisheries and aquaculture has spotlighted the importance of disease for lobster biology, population dynamics and ecology. Researchers, managers, and fishers thus increasingly recognize the need to understand lobster pathogens and parasites so they can be managed proactively and their impacts minimized where possible. At the 2011 International Conference and Workshop on Lobster Biology and Management a special session on lobster diseases was convened and this special issue of Diseases of Aquatic Organisms highlights those proceedings with a suite of articles focused on diseases discussed during that session.

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Homarus

Wahle¹,

Castro²,

Tully³

et al. 2013

Lobsters: Biology, Management, Aquaculture and Fisheries

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Exposures ofHomarus americanusShell to Three Bacteria Isolated from Naturally Occurring Epizootic Shell Disease Lesions

Cited by 58 publications

References 24 publications

Fine-scale transition to lower bacterial diversity and altered community composition precedes shell disease in laboratory-reared juvenile American lobster

Fine-scale transition to lower bacterial diversity and altered community composition precedes shell disease in laboratory-reared juvenile American lobster

Disease effects on lobster fisheries, ecology, and culture: overview of DAO Special 6

Homarus

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