Exposure to traffic exhausts and oxidative DNA damage

Abstract

Aims: To assess the relations between exposure to traffic exhausts and indicators of oxidative DNA damage among highway toll station workers. Methods: Cross-sectional study of 47 female highway toll station workers exposed to traffic exhausts and 27 female office workers as a reference group. Exposure assessment was based on average and cumulative traffic density and a biomarker of exposure, urinary 1-hydroxypyrene-glucuronide (1-OHPG). Urinary 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. Plasma nitric oxide (NO) was measured as an indicator of oxidative stress related to traffic exhaust exposure.Results: The mean concentration of urinary 8-OHdG was substantially higher among the exposed nonsmokers (13.6 mg/g creatinine) compared with the reference non-smokers (7.3 mg/g creatinine; difference 6.3, 95% CI 3.0 to 9.6). The mean concentration of NO among the exposed (48.0 mmol/l) was also higher compared with the reference non-smokers (37.6 mmol/l; difference 10.4, 95% CI 20.4 to 21.2). In linear regression adjusting for confounding, a change in log(8-OHdG) was statistically significantly related to a unit change in log(1-OHPG) (b = 0.372, 95% CI 0.081 to 0.663). Conclusions: Results indicate that exposure to traffic exhausts increases oxidative DNA damage. Urinary 8-OHdG is a promising biomarker of traffic exhaust induced oxidative stress.

Exposure to Heavy Metals and Polycyclic Aromatic Hydrocarbons and DNA Damage in Taiwanese Traffic Conductors

“…A major product with a clear mutagenic potential, deoxyguanosine (dG) in urine, has been commonly used as a biomarker of oxidative stress in studies on ambient air pollution (7)(8)(9)(10). Conversely, urinary 1-hydroxypyrene-glucuronide (1-OHPG), a PAH metabolite, has been used as a biomarker of exposure to PAHs (9,11,12). Urinary metals, including cadmium (Cd), arsenic (As), nickel (Ni), lead (Pb), and copper (Cu), indicate short-term or long-term levels of internal dose of exposure to metals (13).…”

“…Urinary metals, including cadmium (Cd), arsenic (As), nickel (Ni), lead (Pb), and copper (Cu), indicate short-term or long-term levels of internal dose of exposure to metals (13). Most studies showed that exposure to either PAHs or metals could increase levels of urinary 8-oxodG in occupational or environmental fields (7)(8)(9)14). Only a few studies have investigated the effect of their interaction on biomarkers of oxidative stress, but a joint effect between PAHs and metals was not found (5,6).…”

Oxidative DNA Damage Estimated by Plasma 8‐hydroxydeoxyguanosine (8‐OHdG): Influence of 4, 4'‐methylenebis (2‐chloroaniline) Exposure and Smoking

“…Although there was no significant difference in adjusted 8-OHdG levels between the MBOCA-exposed and unexposed groups, this study found that a male gender, a history of smoking, and being older were associated with increased plasma 8-OHdG. The risk factors which have been shown to be associated with elevation of 8-OHdG includes age, gender, smoking, and other lifestyle factors [19][20][21][22][23][24][25] . Our findings were consistent with the previous studies.…”

Exposure to Polycyclic Aromatic Hydrocarbons Associated with Traffic Exhaust: The Increase of Lipid Peroxidation and Reduction of Antioxidant Capacity

“…We recently studied toll workers exposed to high levels of PM 2.5 from traffic exhausts and found elevated levels of urinary 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, and plasma NO compared with unexposed subjects (Lai et al, 2005). We also measured urinary 1-hydroxypyrene-glucuronide (1-OHPG) as a biomarker of PAH exposure and found a statistically significant association between the biomarker of exposure (1-OHPG) and oxidative DNA damage (8-OHdG) (Lai et al, 2004a).…”