Exposure to aflatoxin B1 interferes with locomotion and neural development in zebrafish embryos and larvae

Wu, Tzu‐Chin; Cheng, Ya-Chih; Chen, Pei‐Jen; Huang, Ying-Tzu; Yu, Feng‐Yih; Liu, Biing-Hui

doi:10.1016/j.chemosphere.2018.11.058

Cited by 44 publications

(34 citation statements)

References 32 publications

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“…Prior reports of the embryotoxicity of AFB1 in the zebrafish model [11] determined comparable lethal concentrations (LC 50 = 2.3 µM, versus 1.1 µM in the present study; see Figure 1) for 72 hpf embryos exposed to AFB1 for 24 h and observed similar developmental deformities including, in particular, deformity of the head, tail, and body axis (Figure 2). Micromolar exposure concentrations associated with lethality, and other various developmental endpoints, have been similarly confirmed in the zebrafish embryo model by more recent studies [12], and the time dependence (>72–96 hpf) of zebrafish embryotoxicity at sub-micromolar concentration, as observed in the present study (Figure 1), has been, likewise, very recently reported [13]. Notably, however, in the present study, toxicity was specifically observed with a sequential exposure regime (i.e., 24 h at 24, 48, 72, and 96 hpf; Figure 1), rather than continuous exposure (as in all prior studies), clearly indicating stage-dependent susceptibility (as opposed to possible cumulative toxicity).…”

Section: Discussionsupporting

confidence: 91%

“…Aligned with previous studies [11,12,13], ambient exposure to AFB1 was lethal to zebrafish embryos in the micromolar range (Figure 1). Developmental effects (Figure 2), within this same exposure range, were both quantitatively and qualitatively consistent with these previous studies.…”

Section: Discussionsupporting

confidence: 87%

“…In the present study, we utilized techniques based on nuclear magnetic resonance (NMR), specifically applied to the zebrafish ( Danio rerio ) embryo model, to investigate metabolic changes associated with AFB1 exposure. Embryonic and other early life (e.g., larval) stages of the zebrafish are well established as a toxicological model, in general, and have been specifically shown in several previous studies [11,12,13] to be an effective model for assessment of AFB1 toxicity. It is worth noting that, in addition to contamination of agricultural products, AFB1 is one of the most common contaminants of aquaculture (i.e., fish) feeds [14].…”

Section: Introductionmentioning

confidence: 99%

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NMR-Based Metabolic Profiles of Intact Zebrafish Embryos Exposed to Aflatoxin B1 Recapitulates Hepatotoxicity and Supports Possible Neurotoxicity

Zuberi

Eeza

Matysik

et al. 2019

Toxins

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Aflatoxin B1 (AFB1) is a widespread contaminant of grains and other agricultural crops and is globally associated with both acute toxicity and carcinogenicity. In the present study, we utilized nuclear magnetic resonance (NMR), and specifically high-resolution magic angle spin (HRMAS) NMR, coupled to the zebrafish (Danio rerio) embryo toxicological model, to characterize metabolic profiles associated with exposure to AFB1. Exposure to AFB1 was associated with dose-dependent acute toxicity (i.e., lethality) and developmental deformities at micromolar (≤ 2 µM) concentrations. Toxicity of AFB1 was stage-dependent and specifically consistent, in this regard, with a role of the liver and phase I enzyme (i.e., cytochrome P450) bioactivation. Metabolic profiles of intact zebrafish embryos exposed to AFB1 were, furthermore, largely consistent with hepatotoxicity previously reported in mammalian systems including metabolites associated with cytotoxicity (i.e., loss of cellular membrane integrity), glutathione-based detoxification, and multiple pathways associated with the liver including amino acid, lipid, and carbohydrate (i.e., energy) metabolism. Taken together, these metabolic alterations enabled the proposal of an integrated model of the hepatotoxicity of AFB1 in the zebrafish embryo system. Interestingly, changes in amino acid neurotransmitters (i.e., Gly, Glu, and GABA), as a key modulator of neural development, supports a role in recently-reported neurobehavioral and neurodevelopmental effects of AFB1 in the zebrafish embryo model. The present study reinforces not only toxicological pathways of AFB1 (i.e., hepatotoxicity, neurotoxicity), but also multiple metabolites as potential biomarkers of exposure and toxicity. More generally, this underscores the capacity of NMR-based approaches, when coupled to animal models, as a powerful toxicometabolomics tool.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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NMR-Based Metabolic Profiles of Intact Zebrafish Embryos Exposed to Aflatoxin B1 Recapitulates Hepatotoxicity and Supports Possible Neurotoxicity

Zuberi

Eeza

Matysik

et al. 2019

Toxins

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“…Conversely, the AFT-albumin adduct with a longer half-life of www.bosaljournals/chemint/ editorci@bosaljournals.com 30-60 days, relative stability and minimum variability can be estimated in peripheral blood to measure the long-term AFT exposure (Williams et al, 2004). Results revealed that various factors are responsible for AFTs production and all living organisms are vulnerable for its toxic effects (Asare Bediako et al, 2019;Blankson et al, 2019;Cheng et al, 2019;Dí az Nieto et al, 2019;Frisvad et al, 2019;Gummadidala et al, 2019;Hamza et al, 2019;Magzoub et al, 2019;Mwakinyali et al, 2019;Shahbazi and Shavisi, 2019;Singh and Cotty, 2019;Tsounidi et al, 2019;Wang et al, 2019a;Wang et al, 2019b;Wu et al, 2019;Xie et al, 2019a;Xie et al, 2019b;Yu et al, 2019;Zeng et al, 2019;Zhao et al, 2019;Zhou et al, 2019). So far, there is need to develop the detoxification methods to save food and feed items without harming the nutritional and sensory characteristics.…”

Section: Detection and Exposure Assessment Of Aftsmentioning

confidence: 99%

Aflatoxins biosynthesis, toxicity and intervention strategies: A review

Int¹

2019

Preprint

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Aflatoxins (AFTs) are toxic products of fungal metabolism, associated with serious health consequences and substantial economic losses to agriculture, livestock and poultry sectors, particularly in the developing countries. This review outlines the current information on AFTs in terms of historical background, classification, relative occurrence and co-existence with other mycotoxins in various food commodities. The phenomenon of aflatoxin (AFT) biosynthesis has been elucidated with reference to molecular basis, genetic regulation and factors affecting the AFT production. Moreover, the in vivo disposition kinetics, toxicological action and toxico-pathological consequences of AFTs have also been highlighted. Currently employed strategies for the detection and detoxification of AFTs, biomarkers of exposure assessment, potential economic impact and regulatory considerations regarding the AFTs have been emphasized.

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“…The aflatoxins can be taken up by human customs through the food chain which can impair the health of humans (Boonyaratpalin et al, 2001;El-Sayed and Khalil, 2009). Aflatoxin B 1 (AFB 1 ) is the major mycotoxin that contaminates aquafeeds globally and mostly common in tropical countries and regarded as a causative agent in illnesses and the mortality of aquacultural species (Wu et al, 2019;Murjani, 2003). Aflatoxin might still be a serious concern in aquaculture because of the vast use of plant feedstuffs in diet formulations, and the spread of AFB 1 by lethal deposits in the fish may be a threat to humans as well (El-Sayed and Khalil, 2009;Manning et al, 2005;Raghavan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of aflatoxin contaminated feed on the fingerlings of tilapia (Oreochromis niloticus Linnaeus, 1758)

Ahmed

Baten

Hossain

et al. 2020

Arch. Agric. Environ. Sci.

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Aflatoxin contamination, particularly common in cultured fishes in Asian countries, are considered unsafe both for fish and human health. However, the presence of aflatoxin in cultured fish feed and their effect are still under estimated in Bangladesh. The present study aimed to assess the effects of aflatoxin on growth performance and residues in tilapia (Oreochromis niloticus) fingerlings. Fish feed were treated with several concentration of aflatoxin as 0 ppb (T0, control), 25 ppb (T1), 50 ppb (T2) and 100 ppb (T3) and fed the tilapia fingerlings (n=10) in individual glass aquaria (24×12×12 inch, 105-litre capacity) conditions for 12 weeks. Comparatively higher body length (cm) and weight gain (g) were observed in treatment T0 (1.68 and 4.98) and T1 (1.60 and 5.48) than those of treatment T2 (1.31 and 4.06) and T3 (1.20 and 3.10), respectively. The specific growth rate (SGR) were almost similar in treatment T0 (52%), T1 (51%) and T2 (52%) whereas declined significantly (p<0.05) in T3 (39%). Higher survival rate was also demonstrated in treatment T0 (90%) and T1 (90%) whereas significantly decreased in treatment T2 (60%) and T3 (40%). The residue of aflatoxin was not detected in T0 and T1. On the contrary, the residual effect in tilapia fingerling was evident in T2 and T3 treatment. The findings of the present study revealed that aflatoxin contaminated feed is harmful for the growth performance and survival of O. niloticus fingerlings. Further study is necessary to safeguard the aquaculture production as well as to produce healthy food for human consumption.

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Exposure to aflatoxin B1 interferes with locomotion and neural development in zebrafish embryos and larvae

Cited by 44 publications

References 32 publications

NMR-Based Metabolic Profiles of Intact Zebrafish Embryos Exposed to Aflatoxin B1 Recapitulates Hepatotoxicity and Supports Possible Neurotoxicity

NMR-Based Metabolic Profiles of Intact Zebrafish Embryos Exposed to Aflatoxin B1 Recapitulates Hepatotoxicity and Supports Possible Neurotoxicity

Aflatoxins biosynthesis, toxicity and intervention strategies: A review

Effects of aflatoxin contaminated feed on the fingerlings of tilapia (Oreochromis niloticus Linnaeus, 1758)

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