Exposition of antitumour activity of a chemically characterized exopolysaccharide from a probiotic Lactobacillus plantarum MTCC 9510

Abstract

As majority of chemical compounds identified as anti-cancerous are toxic to normal cells, the discovery and identification of new safe drugs is a necessity in the biomedical field. The antioxidant, antitumour and immunomodulating properties of an exopolysaccharide of sequence -α-D-glucose, α-D-mannose and β-D-glucose-, purified from a probiotic Lactobacillus plantarum was studied. The immunostimulation of the compound in human lymphocytes was seen at a concentration of 0.1 mg/mL with 20% proliferation rate. The antitumour studies by morphological apoptosis determination and 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay on breast adenocarcinoma cell line (MCF-7) exhibited an IC50 of 10 mg/mL for the compound.

Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid beta1–42-induced apoptosis in SH-SY5Y cells

“…The cytotoxic effect of l-EPSs obtained from L. delbrueckii ssp. bulgaricus B3, and L. plantarum GD2 strains on SH-SY5Y cells was in line with the literature 67,68 .…”

“…bulgaricus B3, and L. plantarum GD2 strains on SH-SY5Y cells was in line with the literature 11,12 . l-EPSs at different concentrations alone did not cause any apparent cytotoxicity (data not shown). Ismail and Nampoothiri 67 reported that 50 mg/mL EPS obtained from L. plantarum MTCC 9510 strain had a cytotoxic effect on normal fibroblast cells (L929) at 60%. Dilna et al 68 , according to the study, the EPS obtained from L. plantarum RJF4 strain at a concentration of 500 µg/mL normal fibroblast cells (L6) on the 35% cytotoxic effect was determined.…”

Lactobacillus plantarumMTCC 9510 supplementation protects from chronic unpredictable and sleep deprivation-induced behaviour, biochemical and selected gut microbial aberrations in mice

“…). Furthermore, this strain produces an exopolysaccharide (Ismail and Nampoothiri ), which is well characterized (Ismail and Nampoothiri ) and exhibits anticancer property (Ismail and Nampoothiri ).…”

“…The rational for selecting L. plantarum MTCC 9510 for this study is due to its probiotic attributes which are well established (Aswathy et al 2008). Furthermore, this strain produces an exopolysaccharide (Ismail and Nampoothiri 2010), which is well characterized (Ismail and Nampoothiri 2013) and exhibits anticancer property (Ismail and Nampoothiri 2014).…”

Prophylactic role of Lactobacillus Paracasei exopolysaccharides on colon cancer cells through apoptosis not ferroptosis

“…Researches revealed that these glycosidic components generally are less cytotoxic toward normal cells which makes them a promising candidate in cancer therapy [13,36,37]. In this regard, we carried out experimental assays for evaluating the possible modulatory role of L. paracasei EPS on the molecular targets involved in cancer cell growth, apoptosis, survival, and inflammation signaling pathways.…”