Exploring thermal reversible hydrogels for stem cell expansion in three-dimensions

Shen, Zheyu; Bi, Jian; Shi, Bingyang; Nguyen, D. Q.; Chen, Xian; Zhang, Hu; Dai, Sheng

doi:10.1039/c2sm25407g

Cited by 32 publications

(33 citation statements)

References 36 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example 10 wt.% AAm films with 4.6 mol.% MBA cross-linker have pores that are 5–10 μm in size [32]. NIPAm/AA (acrylic acid) particles with 23 wt.% monomer and 1.9 mol.% MBA cross-linker have a similar cellular structure to the AAm/NIPAm films reported here with a pore size of about 10 μm [37]. …”

Section: Resultsmentioning

confidence: 89%

Protein imprinting in polyacrylamide-based gels

2014

View full text Add to dashboard Cite

Protein imprinting in hydrogels is a method to produce materials capable of selective recognition and capture of a target protein. Here we report on the imprinting of fluorescently-labeled maltose binding protein (MBP) in acrylamide (AAm)/N-isopropylacrylamide (NIPAm) hydrogels. The targeting efficiency and selectivity of protein recognition is usually characterized by the imprinting factor, which in the simplest case is the ratio of protein uptake in an imprinted film divided by the uptake by the corresponding non-imprinted film. Our objective in this work is to study the dynamics of protein binding and elution in imprinted and non-imprinted films to elucidate the processes that control protein recognition. Protein elution from imprinted and non-imprinted films suggests that imprinting results in sites with a distribution of binding energies, and that only a relatively small fraction of these sites exhibit strong binding.

show abstract

Section: Resultsmentioning

confidence: 89%

Protein imprinting in polyacrylamide-based gels

2014

View full text Add to dashboard Cite

show abstract

“…The sol–gel phase change of the nanogels was also observed, which was a solution at room temperature but changed into a soft gel at 37 °C as shown in Figure E. The hydrophobic interactions and electrostatic repulsions between the nanogels resulted in the formation of the hydrogel . Fourier transform infrared (FTIR) spectroscopy analysis was used to identify the functional groups of the nanogel.…”

Section: Resultsmentioning

confidence: 99%

Dynamic Mechanics‐Modulated Hydrogels to Regulate the Differentiation of Stem‐Cell Spheroids in Soft Microniches and Modeling of the Nonlinear Behavior

Zhang

Yang

Abali

et al. 2019

Small

View full text Add to dashboard Cite

Although mechanisms of how physical forces convert into biochemical signals are increasingly understood, it is still unknown how soft cues guide cell behavior. Herein, it is shown that the commitment and differentiation of encapsulating human mesenchymal stem cell (hMSC) spheroids in thermosensitive 3D hydrogels are simply altered by interpenetrating poly(N‐isopropylacrylamide‐co‐2‐hydroxyethyl methacrylate) (NIPAM‐HEMA) nanogel to a gelatin methacryloyl (GelMA) network. This cell‐laden hydrogel provides dynamic mechanics with covalent crosslinking coordinated reversible physical networks, which can regulate hMSCs in situ by reversibly stiffening soft niches via multicyclic temperature changes from 25 to 37 °C. The spreading of hMSC spheroids in the hydrogel is strongly dependent on myosin‐dependent traction stress with dynamic mechanical stimuli through focal adhesion kinase (FAK) signaling. Notably, the dynamic microenvironment gradually influences the expression and distribution from the basal to apical side of nuclear lamin A/C and increases the Yes‐associated protein (YAP) nuclear localization with cycles, which ultimately favors hMSCs undergoing osteogenesis (but not adipogenesis) in the soft microniche. Moreover, it is demonstrated that the viscoelastic behavior of the soft microniche can be guided by temperature through a nonlinear model. These findings highlight the central roles of the dynamic relationship between the biomechanical signals and mechanosensitive transcriptional regulators in cellular mechanosensing.

show abstract

“…It has been demonstrated that this material is able to promote stem cell proliferation and clustering, 37,38 which leads to enhanced cell function and survival rate. 25 The porous structure of the nanogel can maximize mass transport of nutrients, oxygen, and secretion of regenerative factors from the encapsulated cells (Figure 1 and Supporting Information Figure S1).…”

Section: Discussionmentioning

confidence: 99%

Heart Repair Using Nanogel-Encapsulated Human Cardiac Stem Cells in Mice and Pigs with Myocardial Infarction

et al. 2017

Self Cite

View full text Add to dashboard Cite

Stem cell transplantation is currently implemented clinically but is limited by low retention and engraftment of transplanted cells and the adverse effects of inflammation and immunoreaction when allogeneic or xenogeneic cells are used. Here, we demonstrate the safety and efficacy of encapsulating human cardiac stem cells (hCSCs) in thermosensitive poly(N-isopropylacrylamine-co-acrylic acid) or P(NIPAM-AA) nanogel in mouse and pig models of myocardial infarction (MI). Unlike xenogeneic hCSCs injected in saline, injection of nanogel-encapsulated hCSCs does not elicit systemic inflammation or local T cell infiltrations in immunocompetent mice. In mice and pigs with acute MI, injection of encapsulated hCSCs preserves cardiac function and reduces scar sizes, whereas injection of hCSCs in saline has an adverse effect on heart healing. In conclusion, thermosensitive nanogels can be used as a stem cell carrier: the porous and convoluted inner structure allows nutrient, oxygen, and secretion diffusion but can prevent the stem cells from being attacked by immune cells.

show abstract

Exploring thermal reversible hydrogels for stem cell expansion in three-dimensions

Cited by 32 publications

References 36 publications

Protein imprinting in polyacrylamide-based gels

Protein imprinting in polyacrylamide-based gels

Dynamic Mechanics‐Modulated Hydrogels to Regulate the Differentiation of Stem‐Cell Spheroids in Soft Microniches and Modeling of the Nonlinear Behavior

Heart Repair Using Nanogel-Encapsulated Human Cardiac Stem Cells in Mice and Pigs with Myocardial Infarction

Contact Info

Product

Resources

About