Exploring the relationship between cytoplasmic ion content variation and multidrug resistance in cancer cells via ion-release based impedance spectroscopy

Yalçın, Yağmur Demircan; Sukas, Sertan; Töral, Taylan Berkin; Gündüz, Ufuk; Külah, Haluk

doi:10.1016/j.snb.2019.03.084

Cited by 7 publications

(10 citation statements)

References 51 publications

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“…In our previous study 25 , no statistically significant difference was reported between the cytoplasmic ion concentration of CCRF-CEM/wt and CCRF-CEM/doxR cells as clinically relevant MDR model. Using our LOC system with appropriate HCB, we hypothesized that if proper buffer conductivity and frequency are determined, statistically different trapping behavior between CCRF-CEM/wt and CCRF-CEM/doxR cells can be obtained.…”

Section: Methodsmentioning

confidence: 78%

“…The level of MDR (i.e., high-level laboratory or clinically relevant models) may change the electrophysiological properties of cancer cells 32 . We have previously reported that high-level laboratory MDR model, K562/imaR cells, have 1.8 times higher ion concentration than that of K562/wt cells on average 25 . According to the literature, K562/wt cells have cytoplasmic conductivity in the range of 210–240 mS/m 17 .…”

Section: Methodsmentioning

confidence: 94%

“…Sigma Aldrich, TR) in T25 cell culture flasks. Subculturing and drug resistance development procedures were presented in our previous work 25 . The same cells were used in this study after 4 passages.…”

Section: Methodsmentioning

confidence: 99%

“…The same cells were used in this study after 4 passages. Based on IC 50 analyses, K562 cells were classified as high-level laboratory drug resistant models with approximately 60-fold resistance to imatinib as compared to wild type K562 cells, while CCRF-CEM cells were categorized as a clinically relevant model with approximately fourfold resistance to doxorubicin as compared to their wild type progenies 25 , 26 .…”

Section: Methodsmentioning

confidence: 99%

“…To better explain relatively HCB utilization approach, a case study was carried out via MATLAB simulations for Re(f CM ) of K562/wt and K562/imaR cells. The size and electrical properties of cells, previously obtained by Labeed et al and Demircan et al 17 , 25 and presented in Supplementary materials (Sect. 4, Table S1 ), were used in a single shell cell modeling 31 .…”

Section: Methodsmentioning

confidence: 99%

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A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting

Yalçın

Töral²,

Sukas

et al. 2021

Sci Rep

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We report the development of a lab-on-a-chip system, that facilitates coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C), for investigating drug resistance in K562 and CCRF-CEM leukemia cells without (immuno) labeling. Two IM-C units were placed upstream and downstream of the DEP-D unit for enumeration, respectively, before and after the cells were treated in DEP-D unit, where the difference in cell count gave the total number of trapped cells based on their DEP characteristics. Conductivity of the running buffer was matched the conductivity of cytoplasm of wild type K562 and CCRF-CEM cells. Results showed that DEP responses of drug resistant and wild type K562 cells were statistically discriminative (at p = 0.05 level) at 200 mS/m buffer conductivity and at 8.6 MHz working frequency of DEP-D unit. For CCRF-CEM cells, conductivity and frequency values were 160 mS/m and 6.2 MHz, respectively. Our approach enabled discrimination of resistant cells in a group by setting up a threshold provided by the conductivity of running buffer. Subsequent selection of drug resistant cells can be applied to investigate variations in gene expressions and occurrence of mutations related to drug resistance.

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Section: Methodsmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting

Yalçın

Töral²,

Sukas

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

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pH and drug resistance

Koltai¹,

Fliegel²,

Baltazar³

et al. 2023

pH Deregulation as the Eleventh Hallmark of Cancer

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3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Yalçın

Lüttge

2020

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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Using integrated silicon micromachining and thin-film technology, the fabrication of electrically functionalized microsieves for the study of 3D neuronal cell networks in vitro was a major challenge and is still very expensive at the current scale of device production, which is limited to fundamental research. Also, thin-film sidewall electrodes are in contact with the neurons and the microsieves need to be rigorously cleaned prior to reuse or the expensively integrated culture platform must be discarded. To simplify such microsieve studies on neuronal cell networks, we started analysis by optical techniques on polymer microsieves, which also proved to be valuable in our previous studies. Knowing the distribution of cells throughout the pores of the sieve, however, will enhance statistical relevance of these biological experiments. Hence, here, we present the feasibility study on a new technical concept for a cost-effective, fast, and reusable electrical platform to monitor the cell placement distribution in single-use 3D microsieves by a hybrid assembly approach in a label-free manner. The proposed system, having 3D electrodes integrated with microsieves, was compared with the thin-film sidewall electrodes that touch cells in a 3D simulation platform. Although a relatively thick and tapered insulating layer exists between cells and electrodes in the proposed 3D pluggable system, an impedance variation ratio of 3.4% on a measurable based impedance of ∼59 kΩ was obtained in these simulations and is very similar to the values for sidewall electrodes.

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Exploring the relationship between cytoplasmic ion content variation and multidrug resistance in cancer cells via ion-release based impedance spectroscopy

Cited by 7 publications

References 51 publications

A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting

A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting

pH and drug resistance

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

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