Exploring the human urine metabolomic potentialities by comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry

Urinary metabolomic changes as a predictive biomarker of asthma exacerbation

“…1 We 2 and others [3][4][5][6][7] have also reported chronic loss of lung elastic recoil in clinically stable, nonsmoking, adult patients with moderate-to-severe asthma with persistent expiratory airflow limitation that may be partially reversible with treatment. The mechanism(s) responsible for the loss of lung elastic recoil in acute 1 and chronic asthma 2-7 remains unknown, especially with normal diffusing capacity and normal or only mild parenchymal attenuation of lung density on highresolution thin-section lung computed tomography (CT) at full inspiration.…”

“…All samples were analyzed by using solid-phase microextraction (SPME)/GC 3 GC-ToFMS, 4 and samples from 5 of 10 subjects were also analyzed by using 1 H-NMR spectroscopy (see the Methods section in this article's Online Repository). 5 Exploratory principal component analysis (PCA) was applied to GC 3 GC peak areas, as well as to 1 H-NMR spectra, to extract the metabolic features of the exacerbation (see the Methods section in this article's Online Repository).…”

The role of metabolomics in determination of new dietary biomarkers

“…The development of comprehensive 2D GC further enhances separation performance by coupling two columns coated with different stationary phases, which greatly increases separation capacity (32) , chemical selectivity and sensitivity, thus providing more accurate information about metabolite retention times and mass spectra (35) . A recent study by Rocha et al (36) used a 2D-GC-MS untargeted approach to analyse human urine. They identified 700 compounds from a diverse number of chemical families (e.g.…”

Advances in the metabolic profiling of acidic compounds in children’s urines achieved by comprehensive two-dimensional gas chromatography

“…In the same time, GCxGC coupled to time of flight mass spectrometric detection (GCxGC-TOFMS) has been proposed for the separation and semi-quantification of a metabolomic-type mixture with a microwave assisted silylation [9]. The same method was used for the quantitative analysis of amino acid enantiomers in serum and urine [10], as well as for the investigation of the volatile metabolites relative to oxidative stress in smokers' urine [11].…”