Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickens

Zhu, Yong; Zhao, Jingyuan; Wang, Chenxu; Zhang, Fei; Huang, Xinhuo; Ren, Zhouzheng; Yang, Xin; Liu, Yan Li; Yang, Xiaojun

doi:10.1186/s40104-021-00607-w

Cited by 5 publications

(5 citation statements)

References 28 publications

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“…The HPLC procedure utilized in this experiment was more sensitive and accurate for detecting low levels of AA compared to the spectrophotometric analysis, resulting in more reliable results that reflect the dynamic of AA synthesis in broilers. In addition, it is worth noting that chicken embryos have the capacity to synthesize AA in the kidney (metanephros and mesonephros) and yolk sac membrane, with synthesis rates increasing with the development stage [ 23 , 24 ]. The high biosynthesis rate of broilers at hatching explains their high need for AA, as they grow rapidly and are exposed to a complex environment that requires a greater amount of AA to cope with.…”

Section: Discussionmentioning

confidence: 99%

“…The increased AA levels in both livers and spleens indicate the increasing requirement of AA in broilers as they grow. In ovo injection of AA has been reported to have positive effects on posthatch growth and the antioxidant capacity of broilers [ 24 , 33 ]. A previous study also indicated that supplementing broilers’ diets with AA induced lower GLO enzyme activities while increasing concentrations of AA in livers and spleen, resulting in better production performance and enhanced antioxidant ability [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

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Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days

Gan

Zhao

Wang

et al. 2023

Life

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Ascorbic acid (AA) is an indispensable nutrient required to sustain optimal poultry health and performance, which is commonly excluded from the diet of broilers. To investigate the synthesis and distribution of AA during broiler growth and clarify its possible turnover, 144 1 d old healthy Arbor Acres broilers with a body weight of approximately 41 g were randomly assigned to eight groups of 18 broilers each. The kidney, liver, ileum, and spleen of one bird from each group were collected every week until 42 d to detect the synthesis capacity, tissue distribution, and transporter gene expression of AA. The results showed that kidney L-gulonolactone oxidase (GLO) activity responded quadratically (p < 0.001), with maximum activity observed at 7 to 21 d old. Hepatic total AA and dehydroascrobate (DHA) concentration increased linearly (p < 0.001) with age, as did splenic total AA (p < 0.001). In the ileum, mRNA expression of sodium vitamin C transporter 1/2 (SVCT1/2) decreased with the growing age of the broilers (p < 0.05). The expression of SVCT1 in the kidney was not influenced by the growing age of the broilers. The progressive buildup of AA in the liver and spleen of broilers as they age implies an amplified demand for this nutrient. The waning synthesis capacity over time, however, raises concerns regarding the possible inadequacy of AA in the latter growth phase of broilers. The addition of AA to the broilers’ diet might have the potential to optimize their performance. However, the effectiveness of such dietary supplementation requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days

Gan

Zhao

Wang

et al. 2023

Life

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“…The abundance of the transporters and the pattern of mRNA expression of the transporters could dictate the choice of routes. Such as, the yolk sac translates a higher amount of sodium-dependent vitamin C transporter 1 (SVCT1) than amnion and is considered the optimum route for vitamin C inoculation ( 88 ).…”

Section: Routes and Time Of In Ovo Deliverymentioning

confidence: 99%

“…Zhang et al ( 17 ) also reported that IOF of 3 and 6 mg ascorbic acid increased body weight (BW), average daily gain (ADG), and average daily feed intake (ADFI) during the grower phase (Day 14–28), but a high dose (36 mg) negatively impacted growth performance, and 12 mg beneficially decreased plasma Malondialdehyde at the grower and finisher phase of a bird's life ( 17 ). IOF of vitamin C increased plasma level of vitamin C in newly hatched chicks, decreased embryonic mortality, and increased hatchability ( 18 , 88 ).…”

Section: In Ovo Delivered Bioactive Compounds and Their Effectsmentioning

confidence: 99%

In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review

2021

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Early growth and development of the gastrointestinal tract are of critical importance to enhance nutrients' utilization and optimize the growth of poultry. In the current production system, chicks do not have access to feed for about 48–72 h during transportation between hatchery and production farms. This lag time affects early nutrient intake, natural exposure to the microbiome, and the initiation of beneficial stimulation of the immune system of chicks. In ovo feeding can provide early nutrients and additives to embryos, stimulate gut microflora, and mitigate the adverse effects of starvation during pre-and post-hatch periods. Depending on the interests, the compounds are delivered to the embryo either around day 12 or 17 to 18 of incubation and via air sac or amnion. In ovo applications of bioactive compounds like vaccines, nutrients, antibiotics, prebiotics, probiotics, synbiotics, creatine, follistatin, L-carnitine, CpG oligodeoxynucleotide, growth hormone, polyclonal antimyostatin antibody, peptide YY, and insulin-like growth factor-1 have been studied. These compounds affect hatchability, body weight at hatch, physiological functions, immune responses, gut morphology, gut microbiome, production performance, and overall health of birds. However, the route, dose, method, and time of in ovo injection and host factors can cause variation, and thereby inconsistencies in results. Studies using this method have manifested the benefits of injection of different single bioactive compounds. But for excelling in poultry production, researchers should precisely know the proper route and time of injection, optimum dose, and effective combination of different compounds. This review paper will provide an insight into current practices and available findings related to in ovo feeding on performance and health parameters of poultry, along with challenges and future perspectives of this technique.

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“…In ovo feeding (IOF) is a viable method that delivers appropriate nutrients to the amnion at the later stages of the embryo (10,11). Numerous studies have demonstrated that IOF of exogenous nutrients plays a positive role in embryonic development and post-hatching performance in poultry (12)(13)(14)(15). L-arginine (Arg) is a nutritionally essential amino acid for poultry and has many biological and physiological functions (16), one of the primary functions is to synthesize protein for supporting cell growth.…”

Section: Introductionmentioning

confidence: 99%

L-arginine alters myogenic genes expression but does not affect breast muscle characteristics by in ovo feeding technique in slow-growing chickens

Morawong

Molee

et al. 2022

Front. Vet. Sci.

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In ovo feeding (IOF) of nutrients is a viable method for increasing muscle mass through hyperplasia and hypertrophy. The objective of this study was to evaluate the effects of IOF of L-arginine (Arg) on breast muscle weight, muscle morphology, amino acid profile, and gene expression of muscle development in slow-growing chickens. Four hundred eighty fertilized eggs were randomly divided into two groups: the first group was the non-injected control group, and the second group was the Arg group, injected with 1% Arg (0.5 mL) into the amnion on day 18 of incubation. After hatching, 160 birds from each group were randomly divided into four replicates of 40 birds each. This experiment lasted for 63 days. The results showed that IOF of Arg did not affect (P > 0.05) breast muscle weight, muscle morphology, and mRNA expression of mammalian target of rapamycin (mTOR) signaling pathway in slow-growing chickens. However, the amino acid profile of breast muscle was altered (P < 0.05) on the day of hatching (DOH), day 21 (D21), and day 42 (D42) post-hatch, respectively. Myogenic factor 5 (Myf5) mRNA expression was upregulated (P < 0.05) on D21 post-hatch. Myogenic regulator 4 (MRF4) mRNA expression was increased (P < 0.05) on DOH. And myogenin (MyoG) was increased (P < 0.05) on DOH and D21 post-hatch, in the Arg group compared to the control group. Overall, IOF of 1% Arg improved the expression of myogenic genes but did not influence muscle morphology and BMW. These results indicate that in ovo Arg dosage (0.5 mL/egg) has no adverse effect on breast muscle development of slow-growing chickens.

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Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickens

Cited by 5 publications

References 28 publications

Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days

Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days

In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review

L-arginine alters myogenic genes expression but does not affect breast muscle characteristics by in ovo feeding technique in slow-growing chickens

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