Exploring the Antivirulence Activity of Pulverulentone A, a Phloroglucinol-Derivative from Callistemon citrinus Leaf Extract, against Multi-Drug Resistant Pseudomonas aeruginosa

Ismail, Maha M.; Hassan, Mariam; Moawad, Sawsan S.; Okba, Mona M.; Ashour, Rehab M.; Fayek, Nesrin M.; Saber, Fatema R.

doi:10.3390/antibiotics10080907

Cited by 14 publications

(8 citation statements)

References 47 publications

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“…55 Pulverulentone A isolated from Callistemon citrinus leaf extract showed significant antibiofilm potentials against the two tested P. aeruginosa strains (PA-3 and PA-7). 54 In particular, at the concentration of 50 μg/mL, the isolated compound was able to inhibit 54% ± 2.6% and 54% ± 11% of biofilm formation, 59% ± 7.4% and 63% ± 13.3% of EPS production, and 72% ± 6.6% and 55% ± 7.2% of pyocyanin synthesis in PA-7 and PA-3, respectively (Figure 2). These results were then confirmed by a molecular docking study, where pulverulentone A inhibited quorum sensing (QS) gene expression (e.g., MyfR, LasR, and QscR) by binding to the enzyme active sites via hydrophobic interactions and hydrogen bonds.…”

Section: ■ Inhibitory Action Of Phloroglucinol and Derivatives Toward...mentioning

confidence: 92%

“…Pulverulentone A isolated from Callistemon citrinus leaf extract showed significant antibiofilm potentials against the two tested P. aeruginosa strains (PA-3 and PA-7) . In particular, at the concentration of 50 μg/mL, the isolated compound was able to inhibit 54% ± 2.6% and 54% ± 11% of biofilm formation, 59% ± 7.4% and 63% ± 13.3% of EPS production, and 72% ± 6.6% and 55% ± 7.2% of pyocyanin synthesis in PA-7 and PA-3, respectively (Figure ).…”

Section: Inhibitory Action Of Phloroglucinol and Derivatives Toward B...mentioning

confidence: 96%

“…Mechanisms of antibacterial, antibiofilm, and antivirulence activities of PG derivatives. The information was obtained from the literature. − GSH, glutathione; SOD, superoxide dismutase; GPx, glutathione peroxidase; GSSG, glutathione disulfide; ROS, reactive oxygen species. Image of Callistemon citrinus reprinted with permission from ref .…”

Section: Inhibitory Action Of Phloroglucinol and Derivatives Toward B...mentioning

confidence: 99%

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Phloroglucinol and Its Derivatives: Antimicrobial Properties toward Microbial Pathogens

Khan

Tabassum

Bamunuarachchi

et al. 2022

J. Agric. Food Chem.

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Phloroglucinol (PG) is a natural product isolated from plants, algae, and microorganisms. Aside from that, the number of PG derivatives has expanded due to the discovery of their potential biological roles. Aside from its diverse biological activities, PG and its derivatives have been widely utilized to treat microbial infections caused by bacteria, fungus, and viruses. The rapid emergence of antimicrobial-resistant microbial infections necessitates the chemical synthesis of numerous PG derivatives in order to meet the growing demand for drugs. This review focuses on the use of PG and its derivatives to control microbial infection and the underlying mechanism of action. Furthermore, as future perspectives, some of the various alternative strategies, such as the use of PG and its derivatives in conjugation, nanoformulation, antibiotic combination, and encapsulation, have been thoroughly discussed. This review will enable the researcher to investigate the possible antibacterial properties of PG and its derivatives, either free or in the form of various formulations.

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Section: ■ Inhibitory Action Of Phloroglucinol and Derivatives Toward...mentioning

confidence: 92%

Section: Inhibitory Action Of Phloroglucinol and Derivatives Toward B...mentioning

confidence: 96%

Section: Inhibitory Action Of Phloroglucinol and Derivatives Toward B...mentioning

confidence: 99%

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Phloroglucinol and Its Derivatives: Antimicrobial Properties toward Microbial Pathogens

Khan

Tabassum

Bamunuarachchi

et al. 2022

J. Agric. Food Chem.

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“…Phytochemicals have demonstrated different modes of antimicrobial potential [41,42]. Bacterial biofilms are implicated in about 80% of microbial infectious diseases [43].…”

Section: Anti-virulent Activity Of the Tested Extractsmentioning

confidence: 99%

The Untargeted Phytochemical Profile of Three Meliaceae Species Related to In Vitro Cytotoxicity and Anti-Virulence Activity against MRSA Isolates

et al. 2022

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Background: A high mortality rate is associated with about 80% of all infections worldwide, mainly due to antimicrobial resistance. Various antimicrobial and cytotoxic activities have been proposed for Meliaceae species. This study aimed to evaluate the in vitro anti-virulence and cytotoxic effect of the leaf extracts of Aphanamixis polystachya, Toona ciliata and Melia azedarach against five MRSA strains and on three cancer cell lines, followed by biological correlation to their encompassed phytoconstituents. Material and Methods: We explored three plants of this family against a panel of Methicillin-resistant Staphylococcus aureus (MRSA) strains and several cancer cell lines to select the most promising candidates for further in vivo and preclinical studies. The phytochemical composition was evaluated by UHPLC–QTOF–MS untargeted profiling. Cell viability was assessed by SRB assay. Minimum Inhibitory Concentration was carried out by using the agar micro-dilution technique. Inhibition of biofilm formation and preformed biofilm disruption were assessed spectrophotomertically, according to the Sultan and Nabil method (2019). Results: A total of 279 compounds were putatively annotated to include different phytochemical classes, such as flavonoids (108), limonoids/terpenoids (59), phenolic acids (49) and lower-molecular-weight phenolics (39). A. polystachya extract showed the most potent cytotoxic activity against Huh-7, DU-145 and MCF-7 cell lines (IC50 = 3, 3.5 and 13.4 µg mL−1, respectively), followed by M. azedarach, with no effect recorded for T. ciliata extract. Furthermore, both A. polystachya and M. azedarach extracts showed promising anti-virulence and antimicrobial activities, with A. polystachya being particularly active against MRSA. These two latter extracts could inhibit and disrupt the biofilm, formed by MRSA, at sub-lethal concentrations. Interestingly, the extracts inhibited hemolysin-α enzyme, thus protecting rabbit RBCs from lysis. A. polystachya extract reduced the pigmentation and catalase enzyme activity of tested pigmented strains better than M. azedarach at both tested sub-MICs. Consequently, susceptibility of the extract-treated cells to oxidant killing by 200 mM H2O2 increased, leading to faster killing of the cells within 120 min as compared to the extract-non-treated cells, likely due to the lower antioxidant-scavenging activity of cells exhibiting less staphyloxanthin production. Conclusion: These findings suggested that both A. polystachya and M. azedarach natural extracts are rich in bioactive compounds, mainly limonoids, phenolics and oxygenated triterpenoids, which can combat MRSA biofilm infections and could be considered as promising sources of therapeutic cytotoxic, antibiofilm and anti-virulence agents.

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“…From therapeutic prospective, the importance of inhibition pyocyanin production came from their critical role in pathogenicity of P. aeruginosa as virulence factor. It is known that pyocyanin has negative impacts on host organs such central nervous system, cardiovascular system, respiratory system and urological system (Ismail et al, 2021).…”

Section: Ability Of Lactobacillus Spp Filtrate To Inhibit Of Pyocyani...mentioning

confidence: 99%

Expression of rhlR gene in Pseudomonas aeruginosa affected by Lactobacillus spp

2022

pnr

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P. aeruginosa is an opportunistic pathogen causes severe infections due to distinct virulence factors such as pyocyanin. This pigment confers the microbe severity during the infections. This study aimed to evaluate metabolites from Lactobacillus on pyocyanin production. Seven isolates of P. aeruginosa were screened for pyocyanin. All the isolates were produced pyocyanin in rate ranged between 0.47 to 0.82 µg/ml. The isolates were AHL producers with absorbance at 630 nm ranged between 2.26 to 4.88. The culture filtrate from Lactobacillus spp. showed inhibition activity against pyocyanin production. The clinical Lactobacillus spp. was characterised by their higher ability to inhibit pyocyanin production compared to the environmental isolate. There was a clear variation in folding change values in expression of rhlR gene after treatment with Lactobacillus filtrate. Treatment with CF of Lac9 was up-regulated 1.89-Fold the transcription of rhlR gene. The results of this study suggest that there is a clear interaction between P. aeruginosa and Lactobacillus regarding the pyocyanin production.

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Exploring the Antivirulence Activity of Pulverulentone A, a Phloroglucinol-Derivative from Callistemon citrinus Leaf Extract, against Multi-Drug Resistant Pseudomonas aeruginosa

Cited by 14 publications

References 47 publications

Phloroglucinol and Its Derivatives: Antimicrobial Properties toward Microbial Pathogens

Phloroglucinol and Its Derivatives: Antimicrobial Properties toward Microbial Pathogens

The Untargeted Phytochemical Profile of Three Meliaceae Species Related to In Vitro Cytotoxicity and Anti-Virulence Activity against MRSA Isolates

Expression of rhlR gene in Pseudomonas aeruginosa affected by Lactobacillus spp

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