Exploring the anti‐quorum sensing activity of a d‐limonene nanoemulsion for Escherichia coli O157:H7

Abstract

In this study, a d-limonene nanoemulsion was developed by using a spontaneous emulsification method and its potential to inhibit the quorum sensing (QS)-regulated properties of Escherichia coli O157:H7 (E. coli) were revealed. The results in this study showed that d-limonene nanoemulsion inhibited E. coli biofilm formation through the suppression of curli and extracellular polymeric substance (EPS) production without inhibiting cell growth, and decreased swimming and swarming ability. Further analyses showed that d-limonene nanoemulsion interfered with auto-inducer 2 (AI-2) communication and repressed the expression of curli related genes and AI-2 importer genes in E. coli. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1979-1986, 2018.

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

“…Moreover, its long-term stability is also enhanced (Figure 5). In a recent work D-limonene was used to prevent the formation of biofilms on E.coli O157:H7 at sub-lethal doses, by blocking the quorum sensing mediated autoinducer-2 (AI-2) communication and curli-related gene expression (Wang et al, 2018).…”

Inhibitors of Bacterial Swarming Behavior

“…Hereby, both swimming and swarming motilities were repressed. [24] The biosynthesis of the AI-2 signal is carriedo ut through cleavage of S-ribosylhomocysteine by LuxS ( Figure 1). [25] For signal detection, AI-2 is phosphorylated and derepresses transcription of target genes through binding to LsrR.…”

“…[78] Also, b-lactam antibiotics as inhibitors of cell-wall biosynthesis affect virulence and population behavior at concentrations below the MIC.F or example, the third-generation cephalosporin ceftazidime (22)i nhibited virulence of P. aeruginosa PAO1 and PAF97 and reduced swarming motilityb ya round8 0% at 0.9 and 3.7 mm,r espectively (Scheme 2). [80] The antibioticsc efotaxime (23), ciprofloxacin (24), chloramphenicol, and trimethoprim completely blocked swarming of the gram-negative pathogen Salmonella enterica (ser.T yphimurium) at subgrowth inhibitory concentrations of 3.5, 0.02, 6, and 3 mm,r espectively.I nc ontrast, amikacin, colistin, kanamycin,a nd tetracycline did not inhibit swarming of S. enterica (ser.T yphimurium). While cefotaxime (23), ciprofloxacin (24)a nd trimethoprim inhibited polar-chemoreceptora rray assembly of S. enterica (ser.T yphimurium) that is essential for swarming, chloramphenicol inhibited swarming by ad ecrease in flagellation (Scheme 2).…”

“…coli O157:H7 (EHEC). Hereby, both swimming and swarming motilities were repressed . The biosynthesis of the AI‐2 signal is carried out through cleavage of S ‐ribosylhomocysteine by LuxS (Figure ) .…”

Nanoemulsions of Satureja montana Essential Oil: Antimicrobial and Antibiofilm Activity against Avian Escherichia coli Strains

“…Biofilm formation is due to bacterium-bacterium interactions, and associations with higher organisms through intercellular communication, known as quorum sensing (QS) systems [70]. It has been suggested that D-limonene NEs inhibit QS-based virulence phenotypes in E. coli, including biofilm formation, curli and exopolysaccharides production, and swimming and swarming motility [70,71]. From the obtained results it can be hypothesized that SEO alone and, to a greater extent, SEO NEs, exerted an inhibitory activity on strong biofilm producers through modulation of QS molecule production or release, preventing the achievement of the threshold concentration for biofilm development.…”