Explorations of time and electrochemical potential: opportunities for fresh perspectives on signalling proteins

Butt, Julea N.

doi:10.1042/bst20130256

Cited by 3 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that SeGrx1 may function to protect IOZCAS-Spex-II cells from CPT-induced oxidative stress, which confirms previous reports that Grx1 plays important roles in antioxidant defense in insects [26]. A similar result was reported that the levels of ROS are controlled by the activities of Grx2 in mitochondria, which can help to modulate the susceptibility of a cell to apoptosis [27]. Prior studies have noted the importance of Grx2 in mitochondrial redox status, when Grx2 knock-down resulted in increasing the sensitivity to cell death induced by doxorubicin/adriamycin and phenylarsin [28].…”

Section: Rnai Targeting Segrx1 Increased the Cytotoxicity Of Cpt In I...supporting

confidence: 91%

Effect of RNAi Targeting SeGrx1 on the Cytotoxicity and Insecticide Susceptibility of Camptothecin in Spodoptera exigua

Yang

Zhang

et al. 2022

Agriculture

View full text Add to dashboard Cite

Glutaredoxins (Grxs) are a class of small, heat-stable, acidic proteins which have been implied in various biological activities in cells, including the defense against oxidative stress induced by various biotic and abiotic factors. In this paper, the effects of RNAi targeting SeGrx1 on the cytotoxicity and insecticide susceptibility of camptothecin (CPT) in Spodoptera exigua were investigated. Results showed that the cytotoxicity of CPT to the cells of S. exigua is heightened significantly by the silencing of SeGrx1. In the larvae of S. exigua, the mortality was significantly increased compared to CPT-alone treatment group at 120 h after knocking down the SeGrx1 gene. Taken together, our results confirmed that SeGrx1 in S. exigua played an important role in protecting the cells from the cytotoxicity induced by CPT, and the sensitivity of S. exigua larvae to CPT was increased by the silencing of SeGrx1. Our findings might provide basic information for understanding the function of Grxs and a strategy in insect pest control of RNAi technology combined with pesticides.

show abstract

Section: Rnai Targeting Segrx1 Increased the Cytotoxicity Of Cpt In I...supporting

confidence: 91%

Effect of RNAi Targeting SeGrx1 on the Cytotoxicity and Insecticide Susceptibility of Camptothecin in Spodoptera exigua

Yang

Zhang

et al. 2022

Agriculture

View full text Add to dashboard Cite

show abstract

“…Through reversible oxidation and reduction of specific amino acids, often reactive cysteine residues, the mitochondrial “ROS signal” can thus target a wide variety of cellular processes. Redox-dependent signaling does not fall under the dogma of “oxidative stress” and may overall be the more important function of ROS in cells, tissues and organs [6].…”

Section: Introductionmentioning

confidence: 99%

Redox-sensitive signaling in inflammatory T cells and in autoimmune disease

Weyand

Shen

Goronzy

2018

Free Radical Biology and Medicine

View full text Add to dashboard Cite

Reactive oxygen species (ROS) are byproducts of oxygen metabolism best known for their damaging potential, but recent evidence has exposed their role as secondary messengers, which regulate cell function through redox-activatable signaling systems. In immune cells, specifically in T cells, redox-sensitive signaling pathways have been implicated in controlling several functional domains; including cell cycle progression, T effector cell differentiation, tissue invasion and inflammatory behavior. T cells from patients with the autoimmune disease rheumatoid arthritis (RA) have emerged as a valuable model system to examine the functional impact of ROS on T cell function. Notably, RA T cells are distinguished from healthy T cells based on reduced ROS production and undergo "reductive stress". Upstream defects leading to the ROS status of RA T cells are connected to metabolic reorganization. RA T cells shunt glucose away from pyruvate and ATP production towards the pentose phosphate pathway, where they generate NADPH and consume cellular ROS. Downstream consequences of the ROS conditions in RA T cells include insufficient activation of the DNA repair kinase ATM, bypassing of the G2/M cell cycle checkpoint and biased differentiation of T cells into IFN-γ and IL-17-producing inflammatory cells. Also, ROS T cells rapidly invade into peripheral tissue due to dysregulated lipogenesis, excessive membrane ruffling, and overexpression of a motility module dominated by the scaffolding protein Tks5. These data place ROS into a pinnacle position in connecting cellular metabolism and protective versus auto-aggressive T cell immunity. Therapeutic interventions for targeted ROS enhancement instead of ROS depletion should be developed as a novel strategy to treat autoimmune tissue inflammation.

show abstract

Protein Electrochemistry: Questions and Answers

Fourmond¹,

Léger²

2016

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

This chapter presents the fundamentals of electrochemistry in the context of protein electrochemistry. We discuss redox proteins and enzymes that are not photoactive. Of course, the principles described herein also apply to photobioelectrochemistry, as discussed in later chapters of this book. Depending on which experiment is considered, electron transfer between proteins and electrodes can be either direct or mediated, and achieved in a variety of configurations: with the protein and/or the mediator free to diffuse in solution, immobilized in a thick, hydrated film, or adsorbed as a sub-monolayer on the electrode. The experiments can be performed with the goal to study the protein or to use it. Here emphasis is on mechanistic studies, which are easier in the configuration where the protein is adsorbed and electron transfer is direct, but we also explain the interpretation of signals obtained when diffusion processes affect the response.This chapter is organized as a series of responses to questions. Questions 1-5 are related to the basics of electrochemistry: what does "potential" or "current" mean, what does an electrochemical set-up look like? Questions 6-9 are related to the distinction between adsorbed and diffusive redox species. The answers to questions 10-13 explain the interpretation of slow and fast scan voltammetry with redox proteins. Questions 14-19 deal with catalytic electrochemistry, when the protein studied is actually an enzyme. Questions 20, 21 and 22 are general.

show abstract

Explorations of time and electrochemical potential: opportunities for fresh perspectives on signalling proteins

Cited by 3 publications

References 21 publications

Effect of RNAi Targeting SeGrx1 on the Cytotoxicity and Insecticide Susceptibility of Camptothecin in Spodoptera exigua

Effect of RNAi Targeting SeGrx1 on the Cytotoxicity and Insecticide Susceptibility of Camptothecin in Spodoptera exigua

Redox-sensitive signaling in inflammatory T cells and in autoimmune disease

Protein Electrochemistry: Questions and Answers

Contact Info

Product

Resources

About