Exploration of bifurcated electron transfer mechanism in Bacillus cereus for enhanced power generation in double-chambered microbial fuel cells

Sreelekshmy, Bhuvanendran Revamma; Basheer, Rubina; Shibli, S.M.A.

doi:10.1016/j.jece.2022.107601

Cited by 12 publications

(4 citation statements)

References 92 publications

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“…37 S sk and S ku depict the distribution of heights and valleys on the electrode surface. The high S sk values (>0) achieved for G-180, G-320, and G-600 evidence the presence of sharp peaks with round valleys, associated with good gripping and adhesion ability due to the large valleys 36 and determine the extent of bacterial attachment and mass transfer of the substrates. Moreover, all the surface-treated electrodes, irrespective of roughness, achieved Gaussian distribution with S ku ≈ 3, which reveals that the surfaces of all the electrodes have symmetrical topography.…”

Section: Resultsmentioning

confidence: 96%

“…It is clear from the figure that as the grit size of the M-SiC increases, the cumulative surface roughness significantly decreases. In addition to the average roughness ( S a ) value, other parameters, including S q , the root-mean-square surface roughness; S p , the maximum peak height; S v , the maximum pit depth; S t , the maximum peak to valley height; S sk , skewness; and S ku , kurtosis, are used for the characterization of surface topography − and are shown in Figure S2B. The groove height distribution of each electrode is revealed from the roughness curve (along the pink line in Figure ).…”

Section: Resultsmentioning

confidence: 99%

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Microbial-Inspired Surface Patterning for Selective Bacterial Actions for Enhanced Performance in Microbial Fuel Cells

Bijimol

Sreelekshmy

Kumar

et al. 2022

ACS Appl. Bio Mater.

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The performance of any bio-electrochemical system is dependent on the efficiency of electrode−microbial interactions. Surface properties play a focal role in bacterial attachment and biofilm formation on the electrodes. In addition to electrode surface properties, selective bacterial adhesion onto the electrode surface is mandatory to mitigate energy loss due to undesired bacterial interactions on the electrode surface. In the present study, microbial-patterned graphite scaffolds are developed for selective bacterial−electrode interactions. A power density as high as 1105 mW/m 2 is achieved with mG-E (a graphite electrode patterned with Escherichia coli), which is about 3 times higher than that of the pristine graphite electrode (370 mW/m 2 ). Initial mechanical pre-treatment of the graphite electrode, followed by bacterial patterning, results in the formation of a unique cobblestone topography with a tuned surface area of 127.12 m 2 /g. This provides suitable morphology with enhanced active sites for selective bacterial intercalation in graphite layers. This cannot be otherwise achieved by any mechanical or other means. A unique methodology of symbolic regression is adopted to validate a genetic algorithm suitable for predicting a perfect correlation between surface characteristics and electrochemical characteristics with a minimum root-mean-square error of 0.08. The bacterial intercalation onto the graphite electrode causes protuberance of the graphite layers that reduces the surface potential and resistance, leading to high electron transfer. The study presents a unique bacterial-inspired surface patterning on the anode, which is critical for the performance of a microbial fuel cell.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Microbial-Inspired Surface Patterning for Selective Bacterial Actions for Enhanced Performance in Microbial Fuel Cells

Bijimol

Sreelekshmy

Kumar

et al. 2022

ACS Appl. Bio Mater.

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“…are potential electroactive bacteria in MFCs [40,41]. For example, Bacillus cereus is capable of promoting electron transfer by aligning the cytochrome complex and excreting flavin molecules [42]. Geobacter sulfurreducens can obtain electrons from fumarate and solid donors and conduct extracellular electron transfer through type IV conductive pili and c-type cytochromes [43,44].…”

Section: Potential Degrading Bacteria and Electroactive Bacteriamentioning

confidence: 99%

Key genes of electron transfer, the nitrogen cycle and tetracycline removal in bioelectrochemical systems

Zhao,

Qin,

Jing

et al. 2023

Biotechnol Biofuels

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Background Soil microbial fuel cells (MFCs) can remove antibiotics and antibiotic resistance genes (ARGs) simultaneously, but their removal mechanism is unclear. In this study, metagenomic analysis was employed to reveal the functional genes involved in degradation, electron transfer and the nitrogen cycle in the soil MFC. Results The results showed that the soil MFC effectively removed tetracycline in the overlapping area of the cathode and anode, which was 64% higher than that of the control. The ARGs abundance increased by 14% after tetracycline was added (54% of the amplified ARGs belonged to efflux pump genes), while the abundance decreased by 17% in the soil MFC. Five potential degraders of tetracycline were identified, especially the species Phenylobacterium zucineum, which could secrete the 4-hydroxyacetophenone monooxygenase encoded by EC 1.14.13.84 to catalyse deacylation or decarboxylation. Bacillus, Geobacter, Anaerolinea, Gemmatirosa kalamazoonesis and Steroidobacter denitrificans since ubiquinone reductase (encoded by EC 1.6.5.3), succinate dehydrogenase (EC 1.3.5.1), Coenzyme Q-cytochrome c reductase (EC 1.10.2.2), cytochrome-c oxidase (EC 1.9.3.1) and electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) served as complexes I, II, III, IV and ubiquinone, respectively, to accelerate electron transfer. Additionally, nitrogen metabolism-related gene abundance increased by 16% to support the microbial efficacy in the soil MFC, and especially EC 1.7.5.1, and coding the mutual conversion between nitrite and nitrate was obviously improved. Conclusions The soil MFC promoted functional bacterial growth, increased functional gene abundance (including nitrogen cycling, electron transfer, and biodegradation), and facilitated antibiotic and ARG removal. Therefore, soil MFCs have expansive prospects in the remediation of antibiotic-contaminated soil. This study provides insight into the biodegradation mechanism at the gene level in soil bioelectrochemical remediation.

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“…(ii) A multicellular life phase, in which cells are sessile and alive in the biolm (surface attached SA) as a complex assembly of bacterial communities with the biolm. 39,40 Temperature, 39 pH and other environmental factors affect the bacterial metabolism which in turn affect the biolm formation and thereby EET. In addition to the surface biolm, free cells and the soluble biolm also play an important role in EET.…”

Section: Introductionmentioning

confidence: 99%

Strategic regulation of barrier characteristics of biofilms to enhance the extracellular electrogenic performance in MFCs: an electrochemical dynamic evaluation study

Remya

Sreelekshmy

Bijimol

et al. 2023

Sustainable Energy Fuels

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Exploration of bifurcated electron transfer mechanism in Bacillus cereus for enhanced power generation in double-chambered microbial fuel cells

Cited by 12 publications

References 92 publications

Microbial-Inspired Surface Patterning for Selective Bacterial Actions for Enhanced Performance in Microbial Fuel Cells

Microbial-Inspired Surface Patterning for Selective Bacterial Actions for Enhanced Performance in Microbial Fuel Cells

Key genes of electron transfer, the nitrogen cycle and tetracycline removal in bioelectrochemical systems

Strategic regulation of barrier characteristics of biofilms to enhance the extracellular electrogenic performance in MFCs: an electrochemical dynamic evaluation study

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