Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies

Chauhan, Monika; Dhar, Zahid Ahmad; Gorki, Varun; Sharma, Sonia; Koul, Ashwani; Bala, Shashi; Kaur, Ramandeep; Kaur, Sukhbir; Sharma, Manu; Dhingra, Neelima

doi:10.1016/j.phytochem.2022.113525

Cited by 3 publications

(3 citation statements)

References 67 publications

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“…Hence, further isolation and identification of the bioactive compounds in L. camara leaf ethanolic extract and studying their implications on triple negative cancer cells and in vivo models would provide a future platform for the area of breast cancer therapy, and will also reveal the potential and drawbacks of the leaf extract as a future therapeutic agent against breast cancer. In a recent study, it was found that a semi-synthetically modified compound of Lantadene B could reduce tumor incidence, tumor volume, and tumor burden of Swiss albino mice (Chauhan et al, 2023). In another study, it was reported that Lantana camara methanolic leaf extract could repress skin papilloma and reduce the death rate in Swiss albino mice (M Sharma et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…However, derivatization of the bioactive compounds can reduce the level of toxicity. In silico studies by Chauhan et al reported that Lantadenes and their derivatives are safe and non-toxic in humans (Chauhan et al, 2023). Another drawback of plant extracts is their formulation and delivery due to low stability, poor solubility, and limited bioavailability.…”

Section: Discussionmentioning

confidence: 99%

“…In another study, it was reported that methanolic extract of L. camara leaves inhibited the proliferation of Caco cells by downregulating the PI3K/Akt signalling pathway and repressing Cyclin D1 levels via the activation of GSK-3β (El-Din et al, 2022). Molecular docking studies performed by Chauhan and coworkers found that lantadenes and their derivatives have good binding affinity towards NF-kB and IKK-beta, and this binding is responsible for the cytotoxic activity of these compounds in A375 and A431 cell lines (Chauhan et al, 2023). Therefore, it is important to delineate the signalling pathways behind the cytotoxic and anti-migratory activities of Lantana camara leaf ethanolic extract used in this study to understand the mode of action of the extract in MDA-MB-231 triple negative breast cancer cell line.…”

Section: Discussionmentioning

confidence: 99%

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Effect ofLantana camaraethanolic leaf extract on survival and migration of MDA-MB-231 triple negative breast cancer cell line

Pal

Sanyal

Das

et al. 2023

Preprint

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Breast cancer is considered as a leading cause of cancer related death worldwide. The development of chemotherapeutic resistance and the production of undesirable side effects steer the search for new potential candidates with therapeutic implications. Lantana camara has been reported to cure a number of ailments, with few studies showing its cytotoxic effect on breast cancer cell lines. However, the impact of Lantana camara on triple negative breast cancer cells is largely obscure to date. The present study investigated the effect of ethanolic extract of Lantana camara leaves on the triple negative breast cancer cell line, MDA-MB-231. We found that Lantana camara leaf extract induced cytomorphological changes and exhibited a growth inhibitory effect on MDA-MB-231 cells in a dose-dependent manner. The extract was observed to induce G0/G1 cell cycle arrest. Nuclear staining of the cells exposed to Lantana camara leaf extract suggested the presence of condensed nuclei and the result of flow cytometry analysis confirmed cell death by apoptosis. Lantana camara leaf extract also reduced the migration of MDA-MB-231 cells as evidenced by the wound healing assay results. In summary, this study demonstrates the efficacy of the extract to produce growth-inhibitory and anti-migratory effects on MDA-MB-231 cells. Our results thus suggest that Lantana camara leaf extract can be considered a potent source of chemotherapeutic agents for triple-negative breast cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect ofLantana camaraethanolic leaf extract on survival and migration of MDA-MB-231 triple negative breast cancer cell line

Pal

Sanyal

Das

et al. 2023

Preprint

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Effect of Lantana camara Ethanolic Leaf Extract on Survival and Migration of MDA-MB-231 Triple-Negative Breast Cancer Cell Line

Pal,

Sanyal,

Das

et al. 2024

Journal of Herbal Medicine

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Computational design and in vitro assay of lantadene-based novel inhibitors of NS3 protease of dengue virus

Mujwar,

Pal,

Sharma

et al. 2024

Open Chemistry

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Dengue virus (DENV) infection is one of the diseases for which no drug is available for the treatment. The DENV NS2B-NS3 protease is considered to be the prime target for anti-dengue drug development because of its importance in the development of new virus subunits via DENV poly-protein breakdown. Pentacyclic triterpenoids (Lantadenes) from the weed Lantana camara L. and its semi-synthetic congeners have shown a wide array of biological activities in the last two decades. The virtual screening strategy was used on the library of 78 natural and semi-synthetic lantadenes to predict the potent antagonists for the NS2B-NS3 protease enzyme of DENV and their experimental validation by in vitro assay of lead molecules. In the in silico analysis of 78 triterpenoids, two lead molecules (−10.60 and −9.93 kcal/mol) were predicted to be inhibitors of protease (viral) when compared to its reference ligand 1,8-dihydroxy-4,5-dinitroanthraquinone (−5.377 kcal/mol). At the same time, binding affinity, pharmacokinetic, and toxicity profiling, along with molecular dynamics simulations, were studied. The in vitro viral infection inhibition assay inferred that lead molecule 62 exhibited a 60% and 45% reduction in DENV titers at 10 and 5 µM concentrations, respectively. The lead molecule 62 can further be optimized for its pharmacophore and has the potential to be developed as a drug-like molecule.

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Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies

Cited by 3 publications

References 67 publications

Effect ofLantana camaraethanolic leaf extract on survival and migration of MDA-MB-231 triple negative breast cancer cell line

Effect ofLantana camaraethanolic leaf extract on survival and migration of MDA-MB-231 triple negative breast cancer cell line

Effect of Lantana camara Ethanolic Leaf Extract on Survival and Migration of MDA-MB-231 Triple-Negative Breast Cancer Cell Line

Computational design and in vitro assay of lantadene-based novel inhibitors of NS3 protease of dengue virus

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