Exploration of anticancer potency of N(4) thiomorpholinyl isatin/5-haloisatin thiosemicarbazones on coordination to Cu2+ ion

5-Methoxyisatin N(4)-Pyrrolidinyl Thiosemicarbazone (MeOIstPyrd) Restores Mutant p53 and Inhibits the Growth of Skin Cancer Cells, In Vitro

“…The 13 C NMR spectra of the compounds also confirm the presence of various chemically non-equivalent carbons. The thiocarbamide carbon peak, carbonyl, and imine carbons were observed at 180.51–179.18, 162.92–163.95, and 133.48–135.21 ppm, respectively, which are in agreement with earlier reports. ,, The characteristic signal for the imino carbon (CN) establishes the formation of TSCs . The signals at 106.04–120.99 and 158.21–155.78 ppm are due to the carbons of the isatin moiety. , The peaks at 24.17–55.63 ppm are assigned to the N(4)–C methylene carbons, and the peaks at 48.82–51.20 ppm correspond to the methoxy carbon. , …”

“…The formation of isatin TSCs exhibited a band at 1546–1587 cm –1 due to the ν(C=N) stretching vibration, which confirms the formation of Schiff base from thiosemicarbazide and 5-methoxyisatin. 28 , 29 The sharp stretching band at 1676–1695 cm –1 was attributed due to the stretching frequency of the C=O group. 20 , 28 , 30 Medium to weak intensity bands in the 3167–3469 cm –1 regions are assigned to the indole N–H and azomethine N–H stretching.…”

“… 33 Two series of absorption bands at 1298–1319 cm –1 (s, m) and 843–885 cm –1 (m, w) were ascribed to the stretching vibrations of C=S groups. 20 , 28 , 29 The absence of a band in the 2500–2600 cm –1 region characteristic of the thiol group (C–SH) indicated a stable thione form of the isatin TSCs in the solid state. 33 …”

“…The thiocarbamide carbon peak, carbonyl, and imine carbons were observed at 180.51–179.18, 162.92–163.95, and 133.48–135.21 ppm, respectively, which are in agreement with earlier reports. 29 , 31 , 32 The characteristic signal for the imino carbon (C=N) establishes the formation of TSCs. 35 The signals at 106.04–120.99 and 158.21–155.78 ppm are due to the carbons of the isatin moiety.…”

“… 35 The signals at 106.04–120.99 and 158.21–155.78 ppm are due to the carbons of the isatin moiety. 29 , 35 The peaks at 24.17–55.63 ppm are assigned to the N(4)–C methylene carbons, and the peaks at 48.82–51.20 ppm correspond to the methoxy carbon. 29 , 36 …”