Exploiting in vitro potential and characterization of surface modified zinc oxide nanoparticles of Isodon rugosus extract: their clinical potential towards HepG2 cell line and human pathogenic bacteria

Siddiquah, Aisha; Hashmi, Syed Salman; Mushtaq, Sadaf; Renouard, Sullivan; Blondeau, Jean Philippe; Abbasi, Rashda; Hano, Christophe; Abbasi, Bilal Haider

doi:10.17179/excli2018-1327

Cited by 9 publications

(4 citation statements)

References 47 publications

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“…After 15 min on a rocker, the optical density of the solution on the plate was measured at 570 nm with a plate reader (Epoch microplate spectrophotometer, BioTek, Vermont, United States). As a positive control, doxorubicin (57.418 and 63.036 µM HCT-116 and Caco-2 cell lines, respectively) was used ( Siddiquah et al, 2018 ). The experiment was performed three times.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic Potential of Biogenic Zinc Oxide Nanoparticles Synthesized From Swertia chirayita Leaf Extract on Colorectal Cancer Cells

Berehu

Anupriya

Khan

et al. 2021

Front. Bioeng. Biotechnol.

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Chemotherapy side effects, medication resistance, and tumor metastasis impede the advancement of cancer treatments, resulting in a poor prognosis for cancer patients. In the last decade, nanoparticles (NPs) have emerged as a promising drug delivery system. Swertia chirayita has long been used as a treatment option to treat a variety of ailments. Zinc oxide nanoparticles (ZnO-NPs) were synthesized from ethanolic and methanolic extract of S. chirayita leaves. ZnO-NPs were characterized using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron Microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD). Its anti-cancer activities were analyzed using cytotoxicity assays [MTT assay and acridine orange (AO) staining] and quantitative real-time PCR (qRT-PCR) using colorectal cancer (CRC) cells (HCT-116 and Caco-2) and control cells (HEK-293). The ZnO-NPs synthesized from the ethanolic extract of S. chirayita have an average size of 24.67 nm, whereas those from methanolic extract have an average size of 22.95 nm with a spherical shape. MTT assay showed NPs’ cytotoxic potential on cancer cells (HCT-116 and Caco-2) when compared to control cells (HEK-293). The IC50 values of ethanolic and methanolic extract ZnO-NPs for HCT-116, Caco-2, and HEK-293 were 34.356 ± 2.71 and 32.856 ± 2.99 μg/ml, 52.15 ± 8.23 and 63.1 ± 12.09 μg/ml, and 582.84 ± 5.26 and 615.35 ± 4.74 μg/ml, respectively. Acridine orange staining confirmed the ability of ZnO-NPs to induce apoptosis. qRT-PCR analysis revealed significantly enhanced expression of E-cadherin whereas a reduced expression of vimentin and CDK-1. Altogether, these results suggested anti-cancer properties of synthesized ZnO-NPs in CRC.

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Section: Methodsmentioning

confidence: 99%

Cytotoxic Potential of Biogenic Zinc Oxide Nanoparticles Synthesized From Swertia chirayita Leaf Extract on Colorectal Cancer Cells

Berehu

Anupriya

Khan

et al. 2021

Front. Bioeng. Biotechnol.

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show abstract

“…However, peaks were obtained at alkaline pH, from pH 8 to 12, from 360 to 380 nm and it was observed that there was a blue-shift as the pH increased with a sharp peak observed at pH 12. Siddiquah et al [90] synthesised ZnO NPs at a pH of 12 using in vitro-derived Isodon rugosus plant extracts and Zn(CH 3 COO) 2 •2H 2 O. According to the authors, a higher pH favoured a larger number of functional groups from the plant extract biomolecules, which are responsible for the stabilisation of the ZnO NPs, leading to greater stability.…”

Section: Factors Affecting Synthesis Of Lamiaceae-mediated Zno Npsmentioning

confidence: 99%

Antibacterial and Photodegradation of Organic Dyes Using Lamiaceae-Mediated ZnO Nanoparticles: A Review

2022

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The green synthesis of zinc oxide nanoparticles (ZnO NPs) using plant extracts has been receiving tremendous attention as an alternative to conventional physical and chemical methods. The Lamiaceae plant family is one of the largest herbal families in the world and is famous for its aromatic and polyphenolic biomolecules that can be utilised as reducing and stabilising agents during the synthesis of ZnO NPs. This review will go over the synthesis and how synthesis parameters affect the Lamiaceae-derived ZnO NPs. The Lamiaceae-mediated ZnO NPs have been utilised in a variety of applications, including photocatalysis, antimicrobial, anticancer, antioxidant, solar cells, and so on. Owing to their optical properties, ZnO NPs have emerged as potential catalysts for the photodegradation of organic dyes from wastewater. Furthermore, the low toxicity, biocompatibility, and antibacterial activity of ZnO against various bacteria have led to the application of ZnO NPs as antibacterial agents. Thus, this review will focus on the application of Lamiaceae-mediated ZnO NPs for the photodegradation of organic dyes and antibacterial applications.

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“…ZnO-NPs have shown tremendous antibacterial and antifungal activities (Riaz et al, 2018) . Owing to their GRAS (Generally recognized as safe) status as per FDA, the use of ZnO-NPs is preferred in various biological assays (Siddiquah et al, 2018) . ZnO-NPs have also showed numerous applications in drug delivery and diagnostics (Sumaira et al, 2018) .…”

Section: Introductionmentioning

confidence: 99%

Delphinium uncinatum mediated biosynthesis of zinc oxide nanoparticles and in-vitro evaluation of their antioxidant, cytotoxic, antimicrobial, anti-diabetic, anti-inflammatory, and anti-aging activities

Rehman

Ali

Khan

et al. 2023

Saudi Journal of Biological Sciences

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Exploiting in vitro potential and characterization of surface modified zinc oxide nanoparticles of Isodon rugosus extract: their clinical potential towards HepG2 cell line and human pathogenic bacteria

Cited by 9 publications

References 47 publications

Cytotoxic Potential of Biogenic Zinc Oxide Nanoparticles Synthesized From Swertia chirayita Leaf Extract on Colorectal Cancer Cells

Cytotoxic Potential of Biogenic Zinc Oxide Nanoparticles Synthesized From Swertia chirayita Leaf Extract on Colorectal Cancer Cells

Antibacterial and Photodegradation of Organic Dyes Using Lamiaceae-Mediated ZnO Nanoparticles: A Review

Delphinium uncinatum mediated biosynthesis of zinc oxide nanoparticles and in-vitro evaluation of their antioxidant, cytotoxic, antimicrobial, anti-diabetic, anti-inflammatory, and anti-aging activities

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