Objectives: The objectives of this study were to (1) evaluate the extent to which school-age children benefit from fundamental frequency (F0) differences between target words and competing two-talker speech, and (2) assess whether this benefit changes with age. It was predicted that while children would be more susceptible to speech-in-speech masking compared to adults, they would benefit from differences in F0 between target and masker speech. A second experiment was conducted to evaluate the relationship between frequency discrimination thresholds and the ability to benefit from target/masker differences in F0. Design: Listeners were children (5-15 years) and adults (20-36 years) with normal hearing. In the first experiment, speech recognition thresholds (SRTs) for disyllabic words were measured in a continuous, 60-dB-SPL two-talker speech masker. The same male talker produced both the target and masker speech (average F0 = 120 Hz). The level of the target words was adaptively varied to estimate the level associated with 71% correct identification. The procedure was a four-alternative forced-choice with a picture-pointing response. Target words either had the same mean F0 as the masker or it was shifted up by 3, 6 or 9 semitones. To determine the benefit of target/masker F0 separation on word recognition, masking release was computed by subtracting thresholds in each shifted-F0 condition from the threshold in the unshifted-F0 condition. In the second experiment, frequency discrimination thresholds were collected for a subset of listeners to determine whether sensitivity to F0 differences would be predictive of SRTs. The standard was the syllable /ba/ with an F0 of 250 Hz; the target stimuli had a higher F0. Discrimination thresholds were measured using a three-alternative, three-interval forced choice procedure. Results: Younger children (5-12 years) had significantly poorer SRTs than older children (13-15 years) and adults in the unshifted-F0 condition. The benefit of F0 separations generally increased with increasing child age and magnitude of target/masker F0 separation. For 5-to 7-year-olds, there was a small benefit of F0 separation in the 9-semitone condition only. For 8-to 12-year-olds, there was a benefit from both 6-and 9-semitone separations, but to a lesser degree than what was observed for older children (13-15 years