Experimental transscleral freezing at temperatures ranging from −20 to −160°C

Abstract

In a previous article we described the ocular lesions brought about by the application of cones of solid carbon dioxide (-79 ~ C) to the sclera of a rabbit for various periods of time. We compared these lesions with those caused by transscleral diathermy (OOSTERHt3IS, BRlrtAYE & DE RAAN, 1968). From these experiments we concluded that cold application at -79~ gave a good chorioretinal adhesion with fewer secondary destructive lesions than observed after diathermy, such as necrosis of the scleral fibres, vascular thrombosis, haemorrhages and retinal and subretinal oedema.In the present study the experiments were extended to comprise both ophthalmoscopic and histologic examinations of lesions made by cryocoagulation at different temperatures and times of application. The object of this systematic study was to determine the range of temperatures and time at which a good chorioretinal adhesion may be obtained without unwanted side-effects. MATERIAL AND METHOD264 Experiments were carried out on eyes of healthy rabbits weighing approx. 3.5 kg. all having a pigmented iris and fundus. Albinotic eyes were not used because they are known to be less susceptible to cold (DE aOErrH, 1965). All the experiments were carried out according to the surgical technique described previously (1968). For cold application we used the Union Carbide Linde CE-3 cryo-apparatus working on liquid nitrogen.

Experimental Cryoapplication with Variations in the Pressure Exerted on the Sclera

“…and for repeated applications [Oosterhuis et al, 1968]. In a second article we studied cryolesion intensity produced by cold application for a constant time at different temperatures ranging from -20 to -160 C [Brihaye et al, 1970]. …”

Experimental Cryoapplication with Variations in the Pressure Exerted on the Sclera

“…and for repeated applications [Oosterhuis et al, 1968]. In a second article we studied cryolesion intensity produced by cold application for a constant time at different temperatures ranging from -20 to -160 C [Brihaye et al, 1970]. …”

Experimental Cryoapplication with Variations in the Pressure Exerted on the Sclera

“…and for repeated applications [Oosterhuis et al, 1968]. In a second article we studied cryolesion intensity produced by cold application for a constant time at different temperatures ranging from -20 to -160 C [Brihaye et al, 1970]. …”

Experimental Cryoapplication with Variations in the Pressure Exerted on the Sclera

“…and for repeated applications [Oosterhuis et al, 1968]. In a second article we studied cryolesion intensity produced by cold application for a constant time at different temperatures ranging from -20 to -160 C [Brihaye et al, 1970]. …”