Experimental ovarian transplantation on stomach for bone repair in ovariohysterectomized rabbits

Abstract

PURPOSE:To evaluate the bone repair process in ovariohysterectomized rabbit submitted to an ovarian transplant to stomach that may supplying some quantity of estrogen occurs to improve bone healing. METHODS:In 20 female rabbits three holes of 1, 2 and 3mm diameter in tibial shaft were made and after that all animals received OHE through a ventral incision and they were randomly divided into two groups of ten rabbits each. In group one, animals received one of their self-ovaries that transplanted on serosal layer of stomach and group two did not receive treatment. Animals were kept during bone healing for a period of 45 days and radiological, biochemical, biomechanical and histopathological evaluation. RESULTS:The tibial defects in group one healed completely after 45 days and had more callous than second group. There is significant difference between two groups after operation in 21, 28 and 35 days about estrogen, progesterone and phosphatase Alkaline. The maximum forces in group one, were significantly higher than that for the group two. CONCLUSION:Ovarian transplantation prevents the effects of ovariohysterectomized on bone healing of rabbit tibia, suggesting that unilateral transplanted ovaries can substitute for the action of ovaries on the skeleton in ovariohysterectomized animals.

Histomorphometric evaluation, SEM, and synchrotron analysis of the biological response of biodegradable and ceramic hydroxyapatite-based grafts: from the synthesis to the bed application

“…Therefore, to evaluate the biological effect of samples on bone repair, we used tibial defects in rabbits considering the animal size, low cost, and easy management, with a considerable scientific background supporting their use to study biomaterials and the similarity with the mineral bone density from humans [38]. The non-critical bone defect of 2 mm diameter in tibiae followed the ISO 10993-6/2016 since a previous study showed similar repair of a tibial defect of 1.0, 2.0, and 3.0 mm after 45 d [39], supporting the use of 2.0 mm diameter defect. The micrometric spheres (425-600 µm) dispersed quickly in the surgical site, mainly the HA90 and HA5, quickly fragmented by the alginate dissolution, the strong interaction of particles with interstitial fluid and local cells with clastic activities present at the site of perforations.…”