Experimental osteoporosis in sheep – mechanical and histological approach

Kiełbowicz, Zdzisław; Piątek, Anita; Kuropka, Piotr; Mytnik, Ewa; Nikodem, Anna; Bieżyński, Janusz; Skrzypczak, Piotr; Pezowicz, Celina; Kuryszko, Jan; Reichert, Paweł

doi:10.1515/pjvs-2016-0014

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…However, what would be the alternative? The induction of osteoporosis in adult sheep (or dogs) is medically quite an aggressive procedure, including ovariectomy and steroid hormone application over several months, such as is done in the well-known osteoporosis sheep model (Kielbowicz et al, 2016;. Moreover, this model requires a preparation time of almost one year that would render the study extremely expensive, but also introduce some changes in the body physiology [absence of the normal female sex hormones, depression of metabolism due to chronic steroid application, model-induced impaired bone formation capabilities (Egermann et al, 2005;] that would limit the conclusion potential of the study.…”

Section: Discussionmentioning

confidence: 99%

The slow release of BMP-7 at a low dose accelerates dental implant healing in an osteopenic environment

Hunziker

Liu²,

Muff³

et al. 2021

eCM

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The aim of the present study was to investigate in vivo whether bone morphogenetic protein-7 (BMP-7) was able to promote and accelerate dental implant healing at a low dose in an osteopenic environment by using a delayed drug-release system. Skeletally mature Chinese goats, having physiologically osteopenic (osteoporotic-like) facial bones, served as an animal model. Dental implants were provided with a delayed-release drug-delivery system and BMP-7 was applied at three different dosages. The implants, inserted into healed extraction sockets, were removed 1, 2 and 3 weeks after surgery. Quantification of osseointegration and formation of new bone in the peri- implant space were measured histomorphometrically. Data revealed no evidence of any adverse drug effect at or near the implantation sites. After the first postoperative week, bone neoformation was minimal; after the second week, peri-implant bone formation appeared, particularly in the groups with low dosages of BMP-7. After 3 weeks, new-bone volume was the largest in the group with the lowest (near-physiological) dosage of BMP-7, also showing the highest efficacy of BMP-7. Other dosage or release modes were found to be significantly less effective. BMP-7 was highly efficacious in promoting and accelerating bone formation in the peri-implant space in a hostile osteopenic environment if released by a slow-mode mechanism over time at near physiological activities. Therefore, biological functionalisation of dental implants by a high-power osteogenic factor may improve their healing success in hostile bony environments (osteopenia, osteoporosis, bone atrophy etc.).

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Section: Discussionmentioning

confidence: 99%

The slow release of BMP-7 at a low dose accelerates dental implant healing in an osteopenic environment

Hunziker

Liu²,

Muff³

et al. 2021

eCM

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“…Therefore, small ruminant models are important for the study of human osteoporosis (Chavassieux et al 1997, Lill et al 2002a, b, Andreasen et al 2015, Kielbowicz et al 2015, 2016 induced by OVX and with attention to continuous treatment with glucocorticoids to maintain the osteoporotic bone condition (Ding et al 2010). …”

Section: Pre-clinical and Translational Orthopaedic Research Studiesmentioning

confidence: 99%

Bone turnover markers in sheep and goat: A review of the scientific literature

Camassa

Diogo

Sousa

et al. 2017

An. Acad. Bras. Ciênc.

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Bone turnover markers (BTMs) are product of bone cell activity and are generally divided in bone formation and bone resorption markers. The purpose of this review was to structure the available information on the use of BTMs in studies on small ruminants, especially for monitoring their variations related to diet, exercise, gestation and metabolic lactation state, circadian and seasonal variations, and also during skeletal growth. Pre-clinical and translational studies using BTMs with sheep and goats as animal models in orthopaedic research studies to help in the evaluation of the fracture healing process and osteoporosis research are also described in this review. The available information from the reviewed studies was systematically organized in order to highlight the most promising BTMs in small ruminant research, as well as provide a wide view of the use of sheep and goat as animal models in orthopaedic research, type of markers and commercial assay kits with cross-reactivity in sheep and goat, method of sample and storage of serum and urine for bone turnover markers determination and the usefulness and limitations of bone turnover markers in the different studies, therefore an effective tool for researchers that seek answers to different questions while using BTMs in small ruminants.

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“…All animals had at least one-week acclimatization period prior to the OVX. General anesthesia was prevalently induced by intravenous injection of ketamine and propofol and prolonged with isoflurane in oxygen through an endotracheal tube [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. Antibiotic prophylaxis was provided by intravenous injection at the time of anesthesia induction.…”

Section: Resultsmentioning

confidence: 99%

“…Contrastingly, a reduction in estrogens and progesterone levels and an increase in parathormone and cortisol levels were detected only in OVX with methylprednisolone therapy [ 28 ]. Similarly, a reduction in calcium and phosphorus levels, strength, Young’s modulus, compressive strength of bone tissue, and deformation (strain) energy was present in OVX with methylprednisolone group [ 30 ]. After five months, OVX with methylprednisolone was shown to induce bone loss in a short period of time in comparison to OVX alone, increasing CTXI and reducing osteocalcin (OC) [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep

Salamanna

Contartese

Veronesi

et al. 2022

IJMS

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Sheep ovariectomy (OVX) alone or associated to steroid therapy, deficient diet, or hypothalamic–pituitary disconnection has proven to be of critical importance for osteoporosis research in orthopedics. However, the impact of specific variables, such as breed, age, diet, time after OVX, and other variables, should be monitored. Thus, the design of comparative studies is mandatory to minimize the impact of these variables or to recognize the presence of unwanted variables as well as to better characterize bone remodeling in this model. Herein, we conducted a systematic review of the last 10 years on PubMed, Scopus, and Web of Knowledge considering only studies on OVX sheep where a control group was present. Of the 123 records screened, 18 studies were included and analyzed. Results showed that (i) Merino sheep are the most exploited breed; (ii) 5–6 years of age is the most used time for inducing OVX; (iii) ventral midline laparotomy is the most common approach to induce OVX; (iv) OVX associated to steroid therapy is the most widely used osteoporosis model; and (v) success of OVX was mostly verified 12 months after surgery. In detail, starting from 12 months after OVX a significant decline in bone mineral density and in microarchitectural bone parameters as well as in biochemical markers were detected in all studies in comparison to control groups. Bone alteration was also site-specific on a pattern as follows: lumbar vertebra, femoral neck, and ribs. Before 12 months from OVX and starting from 3–5 months, microarchitectural bone changes and biochemical marker alterations were present when osteoporosis was induced by OVX associated to steroid therapy. In conclusion, OVX in sheep influence bone metabolism causing pronounced systemic bone loss and structural deterioration comparable to the situation found in osteoporosis patients. Data for treating osteoporosis patients are based not only on good planning and study design but also on a correct animal use that, as suggested by 3Rs principles and by ARRIVE guidelines, includes the use of control groups to be directly contrasted with the experimental group.

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Experimental osteoporosis in sheep – mechanical and histological approach

Cited by 5 publications

References 14 publications

The slow release of BMP-7 at a low dose accelerates dental implant healing in an osteopenic environment

The slow release of BMP-7 at a low dose accelerates dental implant healing in an osteopenic environment

Bone turnover markers in sheep and goat: A review of the scientific literature

Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep

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