Experimental mixture design as a tool to optimize the growth of variousGanodermaspecies cultivated on media with different sugars

Abstract

The influence of different medium components (glucose, sucrose, and fructose) on the growth of different Ganoderma isolates and species was investigated using mixture design. Ten sugar combinations based on three simple sugars were generated with two different concentrations, namely 3.3% and 16.7%, which represented low and high sugar levels, respectively. The media were adjusted to either pH 5 or 8. Ganoderma isolates (two G. boninense from oil palm, one Ganoderma species from coconut palm, G. lingzhi, and G. australe from tower tree) grew faster at pH 8. Ganoderma lingzhi proliferated at the slowest rate compared to all other tested Ganoderma species in all the media studied. However, G. boninense isolates grew the fastest. Different Ganoderma species were found to have different sugar preferences. This study illustrated that the mixture design can be used to determine the optimal combinations of sugar or other nutrient/chemical components of media for fungal growth.

Effects of Environment and Nutritional Conditions on mycelial growth of Ganoderma boninense

“…The culture conditions for mycelial growth of Ganoderma have been reported for G. applanatum (Jo et al 2009;Jeong et al 2009). Goh et al (2016) studied the influence of different medium components (glucose, sucrose and fructose) on the growth of different Ganoderma species including G. boninense, G. lingzhi and G. australe. However, specific culture conditions for G. boninense has not been reported in the literature.…”

Optimization of Biomass Production of Two Ectomycorrhizal Fungi (Lactarius quieticolor and Rhizopogon roseolus) for the Future Pinus radiata Nursery Inoculation

“…Examples of this include research being conducted on the entomopathogenic fungus Cordyceps militaris and Metarhizium rileyi for biomass production (Hsieh et al, 2009;Cui and Yuan, 2011;Song et al, 2017), saprophytic fungi Lentinus squarrosulus (Ahmad et al, 2013) for mycelium and exopolysaccharide production, Ganoderma lucidum for phenolic compound production and biomass (Zárate et al, 2013;Goh et al, 2016), Pleurotus sp. for enzyme production (Saravanakumar et al, 2010) and Filamentous Fungi for Extracellular Lipase Production (Gaurav et al, 2017).…”