Experimental infection of highly and low pathogenic avian influenza viruses to chickens, ducks, tree sparrows, jungle crows, and black rats for the evaluation of their roles in virus transmission

Hiono, Takahiro; Okamatsu, Masatoshi; Yamamoto, Naoki; Ogasawara, Kohei; Endo, Mayumi; Kuribayashi, Saya; Shichinohe, Shintaro; Motohashi, Yurie; Chu, Duc-Huy; Suzuki, Marina Satika; Ichikawa, Takaya; Nishi, Tatsuya; Abe, Yuri; Matsuno, Keita; Tanaka, Kazuyuki; Tsutomu, Tanigawa; Kida, Hiroshi; Sakoda, Yoshihiro

doi:10.1016/j.vetmic.2015.11.009

Cited by 30 publications

(26 citation statements)

References 28 publications

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“…Overall, MOC-Du were shown, with all three methods, to be less susceptible in vitro for IAV-infection compared to MOC-Ch and MOC-Tu independent of the investigated influenza subtype. In vivo studies previously reported that ducks were less susceptible to LPAIV in comparison to other birds including domestic poultry, jungle crows, and tree sparrows (Hiono et al, 2016); whereas, authors did not investigate the reproductive tract. Possible differences in viral binding and replication of human-adapted virus pH1N1 and avian adapted viruses H9N2 and H6N8, could be the variation in the expression pattern of α2,3 and α2,6 sialic acids in the epithelial layer (Pillai and Lee, 2010).…”

Section: Discussionmentioning

confidence: 99%

Interaction of Influenza A Viruses with Oviduct Explants of Different Avian Species

Sid¹,

Hartmann²,

Winter

et al. 2017

Front. Microbiol.

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Infection of poultry with low pathogenic avian influenza viruses (LPAIV) is often associated with mild respiratory symptoms but may also lead to loss in egg production in laying birds. In vivo susceptibility of the reproductive tract for LPAIV infection was reported for turkeys and chickens, but virus-interaction with epithelial cells of the oviduct and possible stimulation of the local antiviral immune responses have not been characterized. In this study, we wanted to investigate the suitability of magnum organ cultures (MOC) as an in vitro model to study virus-host interactions. We compared the susceptibility of duck (Du), chicken (Ch), and turkey (Tu) MOC for three different influenza A viruses (IAV). Overall, the course of infection and the antiviral immune response varied between strains as well as host cell origin, but MOC gave reproducible results for all investigated parameters within each species. While pandemic (p) H1N1 and H9N2 efficiently replicated in MOC-Ch and MOC-Tu, MOC-Du were significantly less susceptible to infection as indicated by a reduced replication level for both viruses (p < 0.05). Overall, virus replication levels did not correlate with interferonα (IFNα) mRNA-expression levels in neither species. H9N2-infection led to a significant upregulation of interferonλ (IFNλ) mRNA expression in MOC of all species compared to the non-infected controls (p < 0.05), while a correlation with replication levels was only seen for MOC-Tu. pH1N1-infection induced only significant upregulation of IFNλ mRNA expression in MOC-Tu at 48 hours post infection (p < 0.05), but the expression pattern did not correlate with replication levels. Our results show that MOC are a suitable model to study IAV-interaction with the mucosal surface of the avian reproductive tract. The data suggest that the reproductive tract may play a role in the pathobiology of IAV in poultry.

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Section: Discussionmentioning

confidence: 99%

Interaction of Influenza A Viruses with Oviduct Explants of Different Avian Species

Sid¹,

Hartmann²,

Winter

et al. 2017

Front. Microbiol.

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“…In contrast to the virus growth in chickens, growth of A/black swan/Akita/1/2016 (H5N6) virus was slightly lower in ducks than that by A/chicken/Kumamoto/1‐7/2014 (H5N8), which has been studied by Kanehira et al . and Hiono et al . .…”

Section: Discussionmentioning

confidence: 99%

Characterization of H5N6 highly pathogenic avian influenza viruses isolated from wild and captive birds in the winter season of 2016–2017 in Northern Japan

Hiono¹,

Okamatsu²,

Matsuno³

et al. 2017

Microbiology and Immunology

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On 15 November 2016, a black swan that had died in a zoo in Akita prefecture, northern Japan, was strongly suspected to have highly pathogenic avian influenza (HPAI); an HPAI virus (HPAIV) belonging to the H5N6 subtype was isolated from specimens taken from the bird. After the initial report, 230 cases of HPAI caused by H5N6 viruses from wild birds, captive birds, and domestic poultry farms were reported throughout the country during the winter season. In the present study, 66 H5N6 HPAIVs isolated from northern Japan were further characterized. Phylogenetic analysis of the hemagglutinin gene showed that the H5N6 viruses isolated in northern Japan clustered into Group C of Clade 2.3.4.4 together with other isolates collected in Japan, Korea and Taiwan during the winter season of 2016-2017. The antigenicity of the Japanese H5N6 isolate differed slightly from that of HPAIVs isolated previously in Japan and China. The virus exhibited high pathogenicity and a high replication capacity in chickens, whereas virus growth was slightly lower in ducks compared with that of an H5N8 HPAIV isolate collected in Japan in 2014. Comprehensive analyses of Japanese isolates, including those from central, western, and southern Japan, as well as rapid publication of this information are essential for facilitating greater control of HPAIVs.

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“…The tissue tropisms of these isolates in ducks were similar to that of A/duck/Anyang/AVL‐1/2001 (H5N1) as previously reported (Tumpey et al., ). Some HPAIVs replicate systemically and cause mortality; however, ducks are often clinically healthy or mildly diseased when infected with HPAIVs (Kishida et al., ; Hiono et al., ). Factors influencing the clinical course of HPAIV‐infected ducks depend on species, breeds, age and virus strain, and the presence of HPAIV in skeletal muscles is not related to the ability to cause disease in waterfowl (Pantin‐Jackwood & Suarez, ; Pantin‐Jackwood, Suarez, Spackman, & Swayne, ).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of avian influenza viruses from raw poultry products illegally imported to Japan by international flight passengers

Shibata

Hiono

Fukuhara

et al. 2017

Transbound Emerg Dis

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The transportation of poultry and related products for international trade contributes to transboundary pathogen spread and disease outbreaks worldwide. To prevent pathogen incursion through poultry products, many countries have regulations about animal health and poultry product quarantine. However, in Japan, animal products have been illegally introduced into the country in baggage and confiscated at the airport. Lately, the number of illegally imported poultry and the incursion risk of transboundary pathogens through poultry products have been increasing. In this study, we isolated avian influenza viruses (AIVs) from raw poultry products illegally imported to Japan by international passengers. Highly (H5N1 and H5N6) and low (H9N2 and H1N2) pathogenic AIVs were isolated from raw chicken and duck products carried by flight passengers. H5 and H9 isolates were phylogenetically closely related to viruses isolated from poultry in China, and haemagglutinin genes of H5N1 and H5N6 isolates belonged to clades 2.3.2.1c and 2.3.4.4, respectively. Experimental infections of H5 and H9 isolates in chickens and ducks demonstrated pathogenicity and tissue tropism to skeletal muscles. To prevent virus incursion by poultry products, it is important to encourage the phased cleaning based on the disease control and eradication and promote the reduction in contamination risk in animal products.

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Experimental infection of highly and low pathogenic avian influenza viruses to chickens, ducks, tree sparrows, jungle crows, and black rats for the evaluation of their roles in virus transmission

Abstract: 25Highly pathogenic avian influenza viruses (HPAIVs) have spread in both poultry and wild 26

Cited by 30 publications

References 28 publications

Interaction of Influenza A Viruses with Oviduct Explants of Different Avian Species

Interaction of Influenza A Viruses with Oviduct Explants of Different Avian Species

Characterization of H5N6 highly pathogenic avian influenza viruses isolated from wild and captive birds in the winter season of 2016–2017 in Northern Japan

Isolation and characterization of avian influenza viruses from raw poultry products illegally imported to Japan by international flight passengers

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