Experimental Endophthalmitis Phacoanaphylactica in Rabbits

Lens Capsule Perforation Without Inflammation in 4 Rabbits From Intravitreal Injection Studies

“…16,22 Other investigators have determined that it was difficult to experimentally induce phacoclastic endophthalmitis in rabbits simply by disrupting the lens capsule; it was necessary to also introduce mycobacterial components to successfully induce inflammation in this species. 3,24,25,31,35 This supports our finding of a lack of inflammation to released lens protein under aseptic conditions. Phacoantigenic endophthalmitis has been induced experimentally in other laboratory species, including rats, guinea pigs, and mice, but presensitization with lens proteins followed by disruption of the lens capsule was usually required.…”

Lens Capsule Perforation Without Inflammation in 4 Rabbits From Intravitreal Injection Studies

“…16,22 Other investigators have determined that it was difficult to experimentally induce phacoclastic endophthalmitis in rabbits simply by disrupting the lens capsule; it was necessary to also introduce mycobacterial components to successfully induce inflammation in this species. 3,24,25,31,35 This supports our finding of a lack of inflammation to released lens protein under aseptic conditions. Phacoantigenic endophthalmitis has been induced experimentally in other laboratory species, including rats, guinea pigs, and mice, but presensitization with lens proteins followed by disruption of the lens capsule was usually required.…”

Immunopathology of the lens. I. Humoral and cellular immune responses to heterologous lens antigens and their roles in ocular inflammation.

“…This is in keeping with the concept that heterologous antibodies are less pathogenetic than autoantibodies and that previous attempts to produce such inflammation resulted in variable and often unpredictable changes, and never typical granulomatous inflammation around the lens (Verhoeff and Lemoine, 1922;Burky, 1934;Scobee and Slaughter, 1944;Muller, 1952). It is known that the insoluble albuminoid is more potent than soluble lens crystallins in inducing T-cell sensitisation and therefore delayed tissue allergy (Manski, 1973).…”

Review: Age-Related Cataract: Immunity and Lens Epithelium-Derived Growth Factor (LEDGF)

“…Mixing cytotoxic anti-lens Abs with whole lens Ags decreased or eliminated this cytotoxic effect (8). There is precedent for adverse immune reactions to lens proteins; lens-associated uveitis (LAU), a severe inflammatory disorder, has been linked to an autoimmune response to lens proteins which have "leaked" or been traumatically or surgically released from the lens (9,10). LAU is a type III, Arthus-like hypersensitivity reaction, mediated by Ag-Ab complexes, and it also involves the complement cascade (11,12).…”

Lens Capsule Perforation Without Inflammation in 4 Rabbits From Intravitreal Injection Studies

“…16,22 Other investigators have determined that it was difficult to experimentally induce phacoclastic endophthalmitis in rabbits simply by disrupting the lens capsule; it was necessary to also introduce mycobacterial components to successfully induce inflammation in this species. 3,24,25,31,35 This supports our finding of a lack of inflammation to released lens protein under aseptic conditions. Phacoantigenic endophthalmitis has been induced experimentally in other laboratory species, including rats, guinea pigs, and mice, but presensitization with lens proteins followed by disruption of the lens capsule was usually required.…”

Immunopathology of the lens. I. Humoral and cellular immune responses to heterologous lens antigens and their roles in ocular inflammation.

“…This is in keeping with the concept that heterologous antibodies are less pathogenetic than autoantibodies and that previous attempts to produce such inflammation resulted in variable and often unpredictable changes, and never typical granulomatous inflammation around the lens (Verhoeff and Lemoine, 1922;Burky, 1934;Scobee and Slaughter, 1944;Muller, 1952). It is known that the insoluble albuminoid is more potent than soluble lens crystallins in inducing T-cell sensitisation and therefore delayed tissue allergy (Manski, 1973).…”

Review: Age-Related Cataract: Immunity and Lens Epithelium-Derived Growth Factor (LEDGF)

“…Mixing cytotoxic anti-lens Abs with whole lens Ags decreased or eliminated this cytotoxic effect (8). There is precedent for adverse immune reactions to lens proteins; lens-associated uveitis (LAU), a severe inflammatory disorder, has been linked to an autoimmune response to lens proteins which have "leaked" or been traumatically or surgically released from the lens (9,10). LAU is a type III, Arthus-like hypersensitivity reaction, mediated by Ag-Ab complexes, and it also involves the complement cascade (11,12).…”

Lens Capsule Perforation Without Inflammation in 4 Rabbits From Intravitreal Injection Studies

“…16,22 Other investigators have determined that it was difficult to experimentally induce phacoclastic endophthalmitis in rabbits simply by disrupting the lens capsule; it was necessary to also introduce mycobacterial components to successfully induce inflammation in this species. 3,24,25,31,35 This supports our finding of a lack of inflammation to released lens protein under aseptic conditions. Phacoantigenic endophthalmitis has been induced experimentally in other laboratory species, including rats, guinea pigs, and mice, but presensitization with lens proteins followed by disruption of the lens capsule was usually required.…”

Immunopathology of the lens. I. Humoral and cellular immune responses to heterologous lens antigens and their roles in ocular inflammation.

“…This is in keeping with the concept that heterologous antibodies are less pathogenetic than autoantibodies and that previous attempts to produce such inflammation resulted in variable and often unpredictable changes, and never typical granulomatous inflammation around the lens (Verhoeff and Lemoine, 1922;Burky, 1934;Scobee and Slaughter, 1944;Muller, 1952). It is known that the insoluble albuminoid is more potent than soluble lens crystallins in inducing T-cell sensitisation and therefore delayed tissue allergy (Manski, 1973).…”

Review: Age-Related Cataract: Immunity and Lens Epithelium-Derived Growth Factor (LEDGF)

“…Mixing cytotoxic anti-lens Abs with whole lens Ags decreased or eliminated this cytotoxic effect (8). There is precedent for adverse immune reactions to lens proteins; lens-associated uveitis (LAU), a severe inflammatory disorder, has been linked to an autoimmune response to lens proteins which have "leaked" or been traumatically or surgically released from the lens (9,10). LAU is a type III, Arthus-like hypersensitivity reaction, mediated by Ag-Ab complexes, and it also involves the complement cascade (11,12).…”