volume 110, issue 1, P388-396 2021
DOI: 10.1016/j.xphs.2020.09.039
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Abstract: The Breast Cancer Resistance Protein (BCRP) is a key transporter in drug efflux and drug-drug interactions. However, endogenous expression of Multidrug Resistance Protein 1 (MDR1) confounds the interpretation of BCRP-mediated transport in in vitro models. Here we used a CRISPR-Cas9 edited Madin-Darby canine kidney (MDCK) II cell line (MDCK cMDR1-KO ) for stable expression of human BCRP (hBCRP) with no endogenous canine MDR1 (cMDR1) expression (MDCK-hBCRP cMDR1-KO ). Targeted quantitative proteomics verified ex…

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