Exosomes Derived From M2 Macrophages Facilitate Osteogenesis and Reduce Adipogenesis of BMSCs

Li, Ziyi; Wang, Yafei; Li, Shilun; Li, Yukun

doi:10.3389/fendo.2021.680328

Cited by 45 publications

(47 citation statements)

References 46 publications

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“…These findings indicate that EVs from the same phenotype Mφ have different effects on the osteogenic differentiation of BMSCs. M2-EVs increased the expression of miR-690, IRS-1, and TAZ in BMSCs, inhibiting adipogenesis and promoting osteogenesis of BMSCs [25]. EVs with complex cargos have been suggested to play different roles in BMSC differentiation.…”

Section: Effect Of Mc-mφ-derived Evs On Osteogenesismentioning

confidence: 99%

Extracellular Vesicles in Bone Homeostasis: Emerging Mediators of Osteoimmune Interactions and Promising Therapeutic Targets

Huang¹,

Lan²,

Shen³

et al. 2022

Int. J. Biol. Sci.

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An imbalance in bone homeostasis results in bone loss and poor healing in bone diseases and trauma. Osteoimmune interactions, as a key contributor to bone homeostasis, depend on the crosstalk between mesenchymal stem cell-osteoblast (MSC-OB) and monocyte-macrophage (MC-Mφ) lineages. Currently, extracellular vesicles (EVs) are considered to be involved in cell-to-cell communication and represent a novel avenue to enhance our understanding of bone homeostasis and to develop novel diagnostic and therapeutic options. In this comprehensive review, we aim to present recent advances in the study of the effect of MC-Mφ-derived EVs on osteogenesis and the regulatory effects of MSC-OB-derived EVs on the differentiation, recruitment and efferocytosis of Mφ. Furthermore, we discuss the role of EVs as crucial mediators of the communication between these cell lineages involved in the development of common bone diseases, with a focus on osteoporosis, osteoarthritis, bone fracture, and periodontal disease. Together, this review focuses on the apparent discrepancies in current research findings and future directions for translating fundamental insights into clinically relevant EV-based therapies for improving bone health.

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Section: Effect Of Mc-mφ-derived Evs On Osteogenesismentioning

confidence: 99%

Extracellular Vesicles in Bone Homeostasis: Emerging Mediators of Osteoimmune Interactions and Promising Therapeutic Targets

Huang¹,

Lan²,

Shen³

et al. 2022

Int. J. Biol. Sci.

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“…4 Researchers found that there was a relative balance between osteogenic and adipogenic differentiation of MSCs. 5 When the adipogenesis increased, the transferring of MSCs to osteoblasts would decrease which led to bone mass loss. 6 Additionally, some clinical studies demonstrated the phenomenon that the fat fraction was inversely proportional to the bone mineral density of the lumbar vertebra.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Trehalose improves palmitic acid‐induced apoptosis of osteoblasts by regulating SIRT3‐medicated autophagy via the AMPK/mTOR/ULK1 pathway

et al. 2022

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Accumulation of lipid substances decreased the activity of osteoblasts. Trehalose is a typical stress metabolite to form a protective membrane on cell surface which has been demonstrated to regulate lipid metabolism. This activity of Trehalose indicates the potential effect of osteoporosis treatment. Our study aimed to determine the therapeutic effect of Trehalose in high fat-induced osteoporosis. We used palmitic acid (PA) to mimic the state of high fat and observed the apoptosis ratio of osteoblasts increased. After adding Trehalose, the apoptosis ratio decreased obviously. Autophagy is a regulatory means involved in the process of apoptosis.We detected the autophagy protein and found that the expression of Beclin-1, Atg5, and LC3 II increased, and p62 decreased after Trehalose treatment. When adding an autophagy inhibitor (3-MA), the expression of Beclin-1, Atg5, and LC3 II decreased, and p62 increased. These results indicated autophagy was an important factor involved in the preventive effect of Trehalose in PA-induced apoptosis. SIRT3 is a mitochondrial gene that can inhibit apoptosis, which has been reported to promote autophagy. We used SIRT3-siRNA to silence the expression of SIRT3 and found the effect of Trehalose was counteracted. The apoptosis ratio increased and the expression of Beclin-1, Atg5, and LC3 II decreased, p62 increased. Additionally, we also fed the mice with a high-fat diet (HFD) and intragastrical Trehalose. The results showed that Trehalose could inhibit the bone mass loss with HFD. Our study revealed the effect and mechanism of Trehalose in the treatment of osteoporosis.

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“…Exosomes secreted by osteoblasts inhibit the differentiation of osteoclast progenitor cells (Wang Q. et al, 2021). Exosomes derived from M2 macrophages (M2D-Exos) inhibit adipogenesis and promote osteogenesis of BMSCs (Li Z. et al, 2021). Therefore, Nan et al constructed exosomes from miRNA-378-transfected adipose-derived stem cells (ASCs) and cocultured the extracted exosomes with BMSCs and human umbilical vein endothelial cells (HUVECs).…”

Section: Other Types Of Methods To Transfect Mirna Into Cellsmentioning

confidence: 99%

Applications of Nonviral Biomaterials for microRNA Transfection in Bone Tissue Engineering

Zhu

Gu²,

Bian³

et al. 2022

Front. Mater.

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Bone tissue engineering, which involves scaffolds, growth factors, and cells, has been of great interest to treat bone defects in recent years. MicroRNAs (miRNAs or miRs) are small, single-stranded, noncoding RNAs that closely monitor and regulate the signaling pathway of osteoblast differentiation. Thus, the role of miRNAs in bone tissue engineering has attracted much attention. However, there are some problems when miRNAs are directly applied in the human body, including negative charge rejection of the cell membrane, nuclease degradation, immunotoxicity, and neurotoxicity. Therefore, it is necessary to use a suitable carrier to transfect miRNAs into cells. In contrast to viral vectors, nonviral vectors are advantageous because they are less immunogenic and toxic; they can deliver miRNAs with a higher molecular weight; and they are easier to construct and modify. This article reviews the application of different miRNAs or anti-miRNAs in bone tissue engineering and the related signaling pathways when they promote osteogenic gene expression and osteogenic differentiation of target cells. An overview of the properties of different types of nonviral miRNA-transfected biomaterials, including calcium phosphates, nanosystems, liposomes, nucleic acids, silk-based biomaterials, cell-penetrating peptides, bioactive glass, PEI, and exosomes, is also provided. In addition, the evaluations in load efficiency, release efficiency, cell uptake rate, biocompatibility, stability, and biological immunity of nonviral miRNA-transfected biomaterials are given. This article also confirms that these biomaterials stably deliver miRNA to promote osteogenic gene expression, osteogenic differentiation of target cells, and mineralization of the extracellular matrix. Because there are differences in the properties of various nonviral materials, future work will focus on identifying suitable transfection materials and improving the transfection efficiency and biocompatibility of materials.

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Exosomes Derived From M2 Macrophages Facilitate Osteogenesis and Reduce Adipogenesis of BMSCs

Cited by 45 publications

References 46 publications

Extracellular Vesicles in Bone Homeostasis: Emerging Mediators of Osteoimmune Interactions and Promising Therapeutic Targets

Extracellular Vesicles in Bone Homeostasis: Emerging Mediators of Osteoimmune Interactions and Promising Therapeutic Targets

RETRACTED: Trehalose improves palmitic acid‐induced apoptosis of osteoblasts by regulating SIRT3‐medicated autophagy via the AMPK/mTOR/ULK1 pathway

Applications of Nonviral Biomaterials for microRNA Transfection in Bone Tissue Engineering

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