Exosomal RNA-profiling of pleural effusions identifies adenocarcinoma patients through elevated miR-200 and LCN2 expression

Hydbring, Per; Petris, Luigi De; Zhang, Yanming; Brandén, Eva; Koyi, Hirsh; Novak, Metka; Kanter, Lena; Hååg, Petra; Hurley, James; Tadigotla, Vasisht; Zhu, Baoli; Skog, Johan; Viktorsson, Kristina; Ekman, Simon; Lewensohn, Rolf

doi:10.1016/j.lungcan.2018.07.018

Cited by 56 publications

(35 citation statements)

References 22 publications

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“…MiRNAs and lncRNAs can be detected in biological fluids through a biopsy of liquids, such as whole blood (lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) [141] and miRNA-182-5p [142]), serum (lncRNA linc00342 [143], MEG3 [144], miR-96 [145] and miR-30a-5p [146]), plasma (lncRNA linc00152 [147], MALAT1 [148], miR-145 [149], miR-30b [150], miR-25 [151] and miR-425-3p [152]), sputum (lncRNA HOTAIR, H19 [153], miR-21 and miR-210 [154]), and pleural effusion (MALAT1 [155] and miR-200 family [156]) in lung cancer. Furthermore, miRNAs can also be found in bronchoalveolar lavage fluid (miR-126 and miR-Let-7a [157]).…”

Section: Discussionmentioning

confidence: 99%

MiRNAs and LncRNAs: Dual Roles in TGF-β Signaling-Regulated Metastasis in Lung Cancer

Lai

Tang

et al. 2020

IJMS

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Lung cancer is one of the most malignant cancers around the world, with high morbidity and mortality. Metastasis is the leading cause of lung cancer deaths and treatment failure. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs), two groups of small non-coding RNAs (nc-RNAs), are confirmed to be lung cancer oncogenes or suppressors. Transforming growth factor-β (TGF-β) critically regulates lung cancer metastasis. In this review, we summarize the dual roles of miRNAs and lncRNAs in TGF-β signaling-regulated lung cancer epithelial-mesenchymal transition (EMT), invasion, migration, stemness, and metastasis. In addition, lncRNAs, competing endogenous RNAs (ceRNAs), and circular RNAs (circRNAs) can act as miRNA sponges to suppress miRNAs, thereby mediating TGF-β signaling-regulated lung cancer invasion, migration, and metastasis. Through this review, we hope to cast light on the regulatory mechanisms of miRNAs and lncRNAs in TGF-β signaling-regulated lung cancer metastasis and provide new insights for lung cancer treatment.

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Section: Discussionmentioning

confidence: 99%

MiRNAs and LncRNAs: Dual Roles in TGF-β Signaling-Regulated Metastasis in Lung Cancer

Lai

Tang

et al. 2020

IJMS

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“…Hydbring et al compared RNA profiles of effusion exosomes from benign (n = 18) and adenocarcinoma groups (n = 18) and identified 17 miRNAs and 71 mRNAs differentially expressed in the 2 groups. Among the mRNAs, only LCN2 (coding for lipocalin 2) was found to have diagnostic potential.…”

Section: Lung Cancermentioning

confidence: 99%

MicroRNA profiling in serous cavity specimens: Diagnostic challenges and new opportunities

Nicolè

Cappello

Cappellesso

et al. 2019

Cancer Cytopathology

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The cytomorphologic evaluation of serous cavity specimens can quickly determine the cause of an effusion as well as the presence or absence of a neoplastic process. Ancillary tests such as immunohistochemistry and fluorescence in situ hybridization are frequently used to help to definitively identify malignant cells, determine a site of origin, and distinguish between malignant and reactive mesothelial proliferations. In recent years, microRNAs (miRNAs) have been increasingly evaluated in cytologic specimens, including those from serous cavities. The examination of miRNA is attractive because of the stability of miRNA in such specimens and data suggesting that miRNA can provide prognostic and therapeutic information in addition to its role in diagnosis. Furthermore, miRNAs exist within extracellular exosomes, and this allows for their analysis in specimens that contain only rare malignant cells, degenerated cells, or obscuring components. This review discusses the technical aspects of specimen processing for miRNA analysis, recent studies of miRNA expression in malignant serous cavity specimens, and the potential importance of miRNA expression analysis for serous cavity specimens in the near future.

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“…32 Immunotherapy has emerged as a first-line therapeutic option for advanced NSCLC either as a single agent or in combination with chemotherapy for patients without a targetable driver mutation. 33,34 PD-L1 expression in pleural effusions compared with pleural biopsies in the same patient by IHC was studied by Xu et al 35,36 In total, 51 cases were reviewed with no difference detected in expression with a threshold of at least 10% PD-L1 expression (65 vs. 65%). 29 However, a difference was detected when a threshold of >50% expression was utilized (12 vs. 24%, p ¼ 0.007) with higher detection in the pleural biopsy specimen.…”

Section: Molecular Targets In Pleural Effusionsmentioning

confidence: 99%

“…The development of powerful high-throughput molecular tools has just begun to be utilized in MPE including next-generation sequencing, 35,36,[39][40][41][42] proteomics, 5,[41][42][43][44] RNA-based transcriptomics, 5,36,38,[40][41][42][43][44][45][46] and metabolomics. [40][41][42]47 These new tools have revolutionized our approach to molecular studies and hold great promise in the study and utilization of liquid biopsies such as pleural fluid.…”

Section: Molecular Targets In Pleural Effusionsmentioning

confidence: 99%

Molecular Testing on Pleural Fluid Samples

Ronaghi

Cai

2019

Semin Respir Crit Care Med

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Pleural effusions are a common manifestation of both malignant and nonmalignant diseases. The sampling of pleural fluid helps categorize effusions as transudative or exudative and helps differentiate paramalignant from malignant disease. Accurate pleural fluid analysis is critical to the appropriate staging of cancers with significant prognostic and treatment implications. However, the etiology of pleural effusions remains unclear in a significant number of cases after routine thoracentesis and pleural fluid analysis. For malignant pleural effusions, cytologic evaluation of pleural fluid has a relatively low sensitivity. We describe the evolving field of molecular pleural fluid analysis in the setting of malignant disease as an active area of investigation with both diagnostic and therapeutic implications.

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Exosomal RNA-profiling of pleural effusions identifies adenocarcinoma patients through elevated miR-200 and LCN2 expression

Cited by 56 publications

References 22 publications

MiRNAs and LncRNAs: Dual Roles in TGF-β Signaling-Regulated Metastasis in Lung Cancer

MiRNAs and LncRNAs: Dual Roles in TGF-β Signaling-Regulated Metastasis in Lung Cancer

MicroRNA profiling in serous cavity specimens: Diagnostic challenges and new opportunities

Molecular Testing on Pleural Fluid Samples

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