Exosomal miR-10a derived from amniotic fluid stem cells preserves ovarian follicles after chemotherapy

Xiao, Guan-Yu; Cheng, Chi‐Feng; Chiang, Yih-Shien; Cheng, W. T. K.; Liu, I-Hsuan; Wu, Shinn-Chih

doi:10.1038/srep23120

Cited by 138 publications

(131 citation statements)

References 59 publications

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“…Our results showed that the EMVs present in CM are positive for surface mesenchymal antigens CD 90 and CD 29 and exosome related protein CD63. The vesicles are a mixed population of exosomes and shedding vesicles4041. Indeed, in our study the fluorescence microscopic analyses revealed that the isolated EMVs show spherical morphology associated to a different size in both healthy and disease specific cells.…”

Section: Discussionmentioning

confidence: 51%

The secretome of periodontal ligament stem cells from MS patients protects against EAE

Rajan

Giacoppo

Diomede

et al. 2016

Sci Rep

101

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Manipulation of stem cells or stem cells-derived secretome has emerged as a novel alternative therapeutic option for multiple sclerosis (MS). Here we show that human periodontal ligament stem cells (hPDLSCs)-derived conditioned medium (hPDLSCs-CM) and purified exosomes/microvesicles (hPDLSCs-EMVs) obtained from Relapsing Remitting (RR)-MS patients and healthy donors block experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing anti-inflammatory and immunosuppressive effects in spinal cord and spleen, and reverse disease progression by restoring tissue integrity via remyelination in the spinal cord. We show that hPDLSCs-CM and hPDLSCs-EMVs reduce pro-inflammatory cytokines IL-17, IFN-γ, IL-1β, IL-6, TNF-α, and induce anti-inflammatory IL-10. In addition, apoptosis related STAT1, p53, Caspase 3, and Bax expressions were attenuated. Our findings unravel the immunosuppressive effects of hPDLSCs-CM and hPDLSCs-EMVs in EAE mice, and suggest simple alternative autologous source for patient-customized cell-free targeting treatment in MS patients.

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Section: Discussionmentioning

confidence: 51%

The secretome of periodontal ligament stem cells from MS patients protects against EAE

Rajan

Giacoppo

Diomede

et al. 2016

Sci Rep

101

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“…In this study, Bim expression was post‐transcriptionally modulated by miR‐17 and miR‐106b . Of the miRNAs we tested, miR10a , miR‐17 , and miR‐24–1 have been shown to regulate Bim expression in other organ systems: cardiac myocytes (53–55), pancreatic carcinoma (99), ovarian cells (52), lymphocytes (100, 101), or acute lymphocytic leukemia (56). Contrary to our findings, Kan and colleagues (57) did not detect changes in Bim expression in the presence of either the miR‐106b mimic or the miR‐106b inhibitor, probably because of differences in endogenous miRNA expression in different tissues.…”

Section: Discussionmentioning

confidence: 99%

Bimgene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development

et al. 2017

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Low nephron endowment at birth has been associated with an increased risk for developing hypertension and chronic kidney disease. We demonstrated in an earlier study that conditional deletion of the microRNA (miRNA)-processing enzyme Dicer from nephron progenitors results in premature depletion of the progenitors and increased expression of the proapoptotic protein Bim (also known as Bcl-2L11). In this study, we generated a compound mouse model with conditional deletion of both and, to determine the biologic significance of increased Bim expression in -deficient nephron progenitors. The loss of partially restored the number of nephron progenitors and improved nephron formation. The number of progenitors undergoing apoptosis was significantly reduced in kidneys with loss of a single allele, or both alleles, of compared to mutant kidneys. Furthermore, 2 miRNAs expressed in nephron progenitors ( and regulated Bim levels and Together, these data suggest that miRNA-mediated regulation of Bim controls nephron progenitor survival during nephrogenesis, as one potential means of regulating nephron endowment.-Cerqueira, D. M., Bodnar, A. J., Phua, Y. L., Freer, R., Hemker, S. L., Walensky, L. D., Hukriede, N. A., Ho, J. gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development.

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“…In recent years, the use of MSCs as cell therapy for the treatment of POF has been investigated in many studies. MSCs from various sources have been used for this purpose, and it has been found that these cells have different abilities in improving ovarian function (Fu, He, Xie, & Liu, ; Song et al, ; Sun et al, ; Xiao et al, ). In a previous study, a successful treatment of POF mice using MSC transplantation was reported (Lai et al, ; Liu et al, ; Wang et al, ).…”

Section: Discussionmentioning

confidence: 99%

The effects of human menstrual blood stem cells‐derived granulosa cells on ovarian follicle formation in a rat model of premature ovarian failure

Manshadi

Navid

Hoshino

et al. 2018

Microscopy Res & Technique

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Many studies have reported that human endometrial mesenchymal stem cells (HuMenSCs) are capable of repairing damaged tissues. The aim of the present study was to investigate the effects of HuMenSCs transplantation as a treatment modality in premature ovarian failure (POF) associated with chemotherapy‐induced ovarian damage. HuMenSCs were isolated from menstrual blood samples of five women. After the in vitro culture of HuMenSCs, purity of the cells was assessed by cytometry using CD44, CD90, CD34, and CD45 FITC conjugate antibody. Twenty‐four female Wistar rats were randomly divided into four groups: negative control, positive control, sham, and treatment groups. The rat models of POF used in our study were established by injecting busulfan intraperitoneally into the rats during the first estrus cycle. HuMenSCs were transplanted by injection via the tail vein into the POF‐induced rats. Four weeks after POF induction, ovaries were collected and the levels of Amh, Fst, and Fshr expression in the granulosa cell (GC) layer, as well as plasma estradiol (E2) and progesterone (P4) levels were evaluated. Moreover, migration and localization of DiI‐labeled HuMenSCs were detected, and the labeled cells were found to be localized in GCs layer of immature follicles. In addition to DiI‐labelled HuMenSCs tracking, increased levels of expression of Amh and Fshr and Fst, and the high plasma levels of E2 and P4 confirmed that HuMenSC transplantation had a significant effect on follicle formation and ovulation in the treatment group compared with the negative control (POF) group.

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Exosomal miR-10a derived from amniotic fluid stem cells preserves ovarian follicles after chemotherapy

Cited by 138 publications

References 59 publications

The secretome of periodontal ligament stem cells from MS patients protects against EAE

The secretome of periodontal ligament stem cells from MS patients protects against EAE

Bimgene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development

The effects of human menstrual blood stem cells‐derived granulosa cells on ovarian follicle formation in a rat model of premature ovarian failure

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