Exopolysaccharides produced by Pandoraea shows emulsifying and anti-biofilm activities

Sacco, Laís Postai; Castellane, Tereza Cristina Luque; Polachini, Tiago Carregari; Lemos, Eliana Gertrudes de Macedo; Alves, Lúcia Maria Carareto

doi:10.1007/s10965-019-1737-1

Cited by 17 publications

(6 citation statements)

References 47 publications

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“…A broad-range glycolipoprotein, rich in Leu-His-Trp amino acids identified from Acinetobacter indicus M6 has low toxicity and removed 82.5% of biofilm at a concentration of 500 µg/ml (Karlapudi et al, 2020). The EPS from Pandoraea pnomenusa MS5 serves as an anti-biofilm agent against Burkholderia cepacia (Sacco et al, 2019). This surfactant is a heteropolysaccharide, with two functional carbonyls and hydroxyl groups, and has oil-emulsifying capacity.…”

Section: Biosurfactantsmentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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Pathogenic microorganisms and their chronic pathogenicity are significant concerns in biomedical research. Biofilm-linked persistent infections are not easy to treat due to resident multidrug-resistant microbes. Low efficiency of various treatments and in vivo toxicity of available antibiotics drive the researchers toward the discovery of many effective natural anti-biofilm agents. Natural extracts and natural product-based antibiofilm agents are more efficient than the chemically synthesized counterparts with lesser side effects. The present review primarily focuses on various natural anti-biofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and microbial enzymes along with their sources, mechanism of action via interfering in the quorum-sensing pathways, disruption of extracellular polymeric substance, adhesion mechanism, and their inhibitory concentrations existing in literature so far. This study provides a better understanding that a particular natural anti-biofilm molecule exhibits a different mode of actions and biofilm inhibitory activity against more than one pathogenic species. This information can be exploited further to improve the therapeutic strategy by a combination of more than one natural anti-biofilm compounds from diverse sources.

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Section: Biosurfactantsmentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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“…Therefore, the exopolysaccharides are always used in drink, makeup, and drug fabrications as additives and excipients [ 15 , 16 ]. Rheological properties are important characters, which can be employed to evaluate whether the exopolysaccharide can be a good candidate for usage in cosmetics or food [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization and Rheological Study of an Exopolysaccharide Obtained from Fermented Mature Coconut Water with Lipomyces starkeyi

Guo

Chen

et al. 2022

Foods

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The current research aimed to solve the environmental pollution of mature coconut water by Lipomyces starkeyi and provide a study of its high value utilization. The innovation firstly investigated the rheological properties and interface behavior of a crude exopolysaccharide and provided a technical support for its application in food. A response surface methodology was performed to ameliorate the fermentation factors of the new exopolysaccharide with mature coconut water as a substrate, and the consequences suggested that the maximum yield was 7.76 g/L under optimal conditions. Rotary shear measurements were used to study the influence of four variables on the viscosity of the exopolysaccharide solution. The results illustrated that the exopolysaccharide solution demonstrated a shear-thinning behavior and satisfactory thermal stability within the test range. The viscosity of the exopolysaccharide solution was significantly affected by ionic strength and pH; it reached the peak viscosity when the concentration of NaCl was 0.1 mol/L and the pH was neutral. The adsorption behavior of the exopolysaccharide at the medium chain triglyceride–water interface was investigated by a quartz crystal microbalance with a dissipation detector. The results demonstrated that the exopolysaccharide might form a multilayer adsorption layer, and the thickness of the adsorption layer was at its maximum at a concentration of 1.0%, while the interfacial film was the most rigid at a concentration of 0.4%. Overall, these results suggest that the exopolysaccharide produced by Lipomyces starkeyi is an excellent biomaterial for usage in drink, makeup and drug fabrications as a thickening and stabilizing agent.

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“…MOE6-EPS also exhibited a noticeable emulsifying activity and formed consistent emulsions (>50% of emulsification index) with various vegetable oils and hydrocarbons at an EPS concentration up to 4 mg/mL (Figure 5). After 24 h more than 50% of the original emulsion volume was retained and the high emulsification index reveals the high stability of the emulsions [95]. Within the hydrocarbons we tested, the variation in emulsion indices may be explained by the lipid composition of the oils.…”

Section: Discussionmentioning

confidence: 93%

Bioremediation Potential of Streptomyces sp. MOE6 for Toxic Metals and Oil

et al. 2021

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Toxic metal contamination has serious effects on human health. Crude oil that may contain toxic metals and oil spills can further contaminate the environment and lead to increased exposure. This being the case, we chose to study the bio-production of inexpensive, environmentally safe materials for remediation. Streptomyces sp. MOE6 is a Gram-positive, filamentous bacterium from soil that produces an extracellular polysaccharide (MOE6-EPS). A one-factor-at-a-time experiments showed that the maximum production of MOE6-EPS was achieved at 35 °C, pH 6, after nine days of incubation with soluble starch and yeast extract as carbon sources and the latter as the nitrogen source. We demonstrated that MOE6-EPS has the capacity to remove toxic metals such as Co(II), Cr(VI), Cu(II) and U(VI) and from solution either by chelation and/or reduction. Additionally, the bacterium was found to produce siderophores, which contribute to the removal of metals, specifically Fe(III). Additionally, purified MOE6-EPS showed emulsifying activities against various hydrophobic substances, including olive oil, corn oil, benzene, toluene and engine oil. These results indicate that EPS from Streptomyces sp. MOE6 may be useful to sequester toxic metals and oil in contaminated environments.

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Exopolysaccharides produced by Pandoraea shows emulsifying and anti-biofilm activities

Cited by 17 publications

References 47 publications

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Optimization and Rheological Study of an Exopolysaccharide Obtained from Fermented Mature Coconut Water with Lipomyces starkeyi

Bioremediation Potential of Streptomyces sp. MOE6 for Toxic Metals and Oil

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