Exoglycosidase markers of diseases

Abstract

Exoglycosidases are hydrolases involved in lysosomal degradation of oligosaccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). In tissues and body fluids, a higher exoglycosidase specific activity is found in N-acetyl-β-hexosaminidase, than β-glucuronidase, α-L-fucosidase, β-galactosidase, α-mannosidase and α-glucosidase. Determination of exoglycosidases (especially N-acetyl-β-hexosaminidase and β-glucuronidase) in body fluids could be an inexpensive, easy to perform and sensitive test for pathological evaluation, as well as in screening and monitoring many diseases, including alcohol abuse, risk of arteriosclerosis, bacterial infections (e.g. Lyme borreliosis), chronic inflammatory processes, such as rheumatoid arthritis and juvenile idiopathic arthritis, asthma, autoimmune hepatitis and primary biliary cirrhosis, as well as cancers.

Isoenzymes A and B of N-acetyl-β-D-hexosaminidase in tissue of colon cancer

“…Wydaje się, że podobnie jak inne endo- [8] i egzoglikozydazy [9,10] mogą one uczestniczyć w niszczeniu tkanki przez guz, wpływać na interakcje komórka-komórka i/lub degradację macierzy wewnątrzkomórkowej [11,12]. Zdaniem Lewa i wsp.…”

The profile of lysosomal exoglycosidases in replicative and stress-induced senescence in early passage human fibroblasts

“…N-acetylglucosaminidase hydrolyzes the bond between N-actetylglucosamine at the non-reducing end of the remaining part of the oligosaccharide and a mannose [11]. The final stage in the catabolism of glycoproteins is the mannosidase action to release the rest of the a and b mannose at the non-reducing end of the oligosaccharide [12].…”

Urinary Fructose-1,6-Bisphosphatase (FBP-1,6) and N-Acetyl-β-Hexosaminidase (HEX) in Monitoring Kidney Transplantation - Literature Review

“…N-acetyl--hexosaminidase (HEX) (EC 3.2.1.52) is the most active of lysosomal exoglycosidases (Popko et al, 2008), taking part in the catabolism of oligosaccharide chains of all types of glycoconjugates (Zwierz et al, 1999). The fact that HEX has the highest activity of amongst exoglycosidases may be explained by the fact that aminohexoses (Nacetyl-glucosamine and N-acetyl-galactosamine) are components of all types of glycoconjugates: glycoproteins, glycolipids and glycosaminoglycans of proteoglycans (Chojnowska et al, 2011). HEX releases monomers of N-acetyl-glucosamine and N-acetylgalactosamine from the non reducing ends of oligosaccharide chains of glycoconjugates (Zwierz et al, 1999).…”

“…In order to correctly reference this scholarly work, feel free to copy and paste the following: Alina Kepka, Sławomir Dariusz Szajda, Napoleon Waszkiewicz, Sylwia Chojnowska, Paweł Pludowski, Jerzy Robert Ładny and Krzysztof Zwierz (2011). Urinary Fructose-1,6-Bisphosphatase (FBP-1,6) and N-Acetyl-β-Hexosaminidase (HEX) in Monitoring Kidney Transplantation -Literature Review, Kidney Transplantation -…”